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  4. Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

  • 中文名稱：
    小鼠腸脂肪酸結(jié)合蛋白(iFABP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08025m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠腸脂肪酸結(jié)合蛋白(iFABP)酶聯(lián)免疫試劑盒（CSB-E08025m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的iFABP含量。iFABP即腸脂肪酸結(jié)合蛋白，是一種小分子胞內(nèi)蛋白。在背景上它與腸道脂肪酸攝取、轉(zhuǎn)運(yùn)等密切相關(guān)。研究機(jī)制方面，其可作為腸道損傷早期敏感標(biāo)志物，還能參與脂肪酸代謝，影響細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)及基因表達(dá)等。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于腸道疾病動(dòng)物模型研究，例如腸炎、缺血再灌注損傷等病理機(jī)制探索，也可用于體外細(xì)胞實(shí)驗(yàn)中評(píng)估藥物或外界刺激對(duì)腸道上皮細(xì)胞的影響，為腸道屏障功能、代謝調(diào)控等基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Fabp2 ELISA Kit; FabpiFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
  • 縮寫：
    iFABP
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 102  
    Range % 96-108  
    1:200 Average % 95  
    Range % 91-99  
    1:400 Average % 96  
    Range % 91-102  
    1:800 Average % 86  
    Range % 82-92  
  • 回收率：
    The recovery of mouse iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 82-94  
    EDTA plasma (n=4) 96 90-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.217 2.317 2.267 2.163  
    200 1.367 1.467 1.417 1.313  
    100 0.777 0.797 0.787 0.683  
    50 0.412 0.422 0.417 0.313  
    25 0.265 0.268 0.267 0.163  
    12.5 0.189 0.199 0.194 0.090  
    6.25 0.162 0.165 0.164 0.060  
    0 0.104 0.103 0.104 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • 基因功能參考文獻(xiàn)：
    1. This direct comparison provides evidence that LFABP and IFABP have distinct roles in intestinal lipid metabolism; differential intracellular functions in intestine and in liver PMID: 23990461
    2. detected the common as well as sex-differential pathways that are modified due to the loss of Fabp2. These findings suggest that the pathways involved in nutrient and xenobiotic metabolism in the intestine are regulated by sex-specific mechanisms PMID: 22584290
    3. loss of I-FABP renders male mice sensitive to high fat diet-induced fatty liver, and this effect is independent of hepatic L-FABP PMID: 17905650
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Calycin superfamily, Fatty-acid binding protein (FABP) family
  • 組織特異性：
    Expressed in the small intestine. Highest expression levels in the proximal ileum.
  • 數(shù)據(jù)庫(kù)鏈接：


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