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  4. Mouse hydroxyproline,Hyp ELISA Kit

Mouse hydroxyproline,Hyp ELISA Kit

  • 中文名稱：
    小鼠羥脯氨酸(Hyp)酶聯免疫試劑盒
  • 貨號：
    CSB-E08839m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠羥脯氨酸(Hyp)酶聯免疫試劑盒（CSB-E08839m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Hyp含量。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，在纖維化疾病研究、組織修復機制探索及骨骼發育相關科研領域具有重要價值；為抗纖維化藥物篩選、組織工程研究及代謝性疾病機制分析提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    Hyp
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hyp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 82-97  
    1:40 Average % 98  
    Range % 91-104  
    1:80 Average % 98  
    Range % 96-105  
    1:160 Average % 97  
    Range % 92-102  
  • 回收率：
    The recovery of mouse Hyp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-101  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    500 2.656 2.755 2.706 2.553  
    250 2.088 2.187 2.138 1.985  
    125 1.492 1.518 1.505 1.352  
    62.5 0.841 0.850 0.846 0.693  
    31.25 0.506 0.515 0.511 0.358  
    15.6 0.337 0.349 0.343 0.190  
    7.8 0.262 0.271 0.267 0.114  
    0 0.152 0.153 0.153 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

We are interested in sourcing Human, mouse, rat hydroxyproline,Hyp ELISA Kit from CusaBio. What would be our purchasing price for it?
Also, can you kindly clarify why they are a species specific elisa kit while hydroxyproline is a small molecule?

A:
Thanks for your inquiry.
I will ask our distributor in your area to give you a quote by email.
Hydroxyproline is small molecule and it is the same in different species. There are 3 reasons why we have species specific elisa kits.
1. For different species, the sample matrix is different and the characteristics of antibody used in the kits are also different.
2. The methods of these 3 kits are different. Human kit and mouse kit are based on double antibody sandwich method.
When the kit was developed, it was designed to mimic the presence of hydroxyproline in vivo. It can detect polymerized form. (including the polymerized form with other proteins and self-polymerization form). For human kit, capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody; For mouse kit, capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
Rat kit is based on competitive method. The plate is coated with mouse monoclonal antibody. HRP conjugated HYP competes with the HYP in the sample. The kit can detect the endogenous total HYP, including free form and polymerized form.
3. The reason why different kits use different methods is based on a variety of factors. You can choose the appropriate kit to avoid matrix problems.

Pls let me know if you have any further questions. Thank you.


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