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Mouse complement factor H,CFH ELISA Kit

  • 中文名稱:
    小鼠補體因子H(CFH)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08933m
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠補體因子H(CFH)酶聯(lián)免疫試劑盒(CSB-E08933m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CFH含量。CFH即補體因子 H,是補體替代途徑的重要調(diào)節(jié)蛋白。其在維持補體系統(tǒng)平衡、保護宿主細胞免受補體攻擊等方面有重要作用。研究機制上,主要圍繞其與補體成分相互作用,調(diào)節(jié)補體激活過程展開,異常時可能引發(fā)多種疾病。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL,該產(chǎn)品可廣泛應(yīng)用于小鼠模型研究中CFH蛋白的動態(tài)監(jiān)測,例如自身免疫性疾病機制探索、感染模型補體通路分析、腎臟或眼部疾病相關(guān)研究等領(lǐng)域,為評估基因敲除/過表達模型、藥物干預(yù)效果或病理進程中的補體調(diào)控機制提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Cfh ELISA kit; Hf1Complement factor H ELISA kit; Protein beta-1-H ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 ng/mL-2000 ng/mL
  • 靈敏度:
    7.81 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CFH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %92
    Range %88-95
    1:2000Average %98
    Range %93-101
    1:4000Average %87
    Range %84-90
    1:8000Average %98
    Range %92-102
  • 回收率:
    The recovery of mouse CFH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9388-97
    EDTA plasma (n=4)9893-101
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    20002.552 2.613 2.583 2.466
    10002.028 2.007 2.018 1.901
    5001.507 1.513 1.510 1.393
    2500.815 0.822 0.819 0.702
    1250.497 0.488 0.493 0.376
    62.50.295 0.287 0.291 0.174
    31.250.208 0.221 0.215 0.098
    00.116 0.118 0.117
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed.
  • 基因功能參考文獻:
    1. this study shows that complement regulatory protein Factor H is a soluble prion receptor that potentiates peripheral prion pathogenesis PMID: 29070671
    2. Factor H and Crry are critical for regulating complement activation at distinct anatomic sites within the kidney. PMID: 27165610
    3. VEGF inhibition decreases local CFH and other complement regulators in the eye and kidney through reduced VEGFR2/PKC-alpha/CREB signaling. PMID: 27918307
    4. environmental factors can drive retinal disease in these mice when linked to complement deficits impairing immune function. Both groups of mice had similar levels of retinal amyloid beta accumulation. Consequently there is no direct link between this and inflammation in Cfh(-/-) mice. PMID: 27397653
    5. role for serum FH levels in the host response to invasive pneumococcal infections PMID: 26802141
    6. absence of plasma CfH conferred susceptibility to glomerulonephritis PMID: 25565310
    7. This new understanding of the complicated interactions of CFH in AMD-like pathology provides an improved foundation for the development of targeted therapies for AMD PMID: 25991857
    8. data suggest that altered interactions of Cfh with MDA-modified proteins may be relevant in explaining the effects of the Cfh variant. PMID: 26030099
    9. Cfh and Cfhr2 genes are expressed in the mouse outer retina. Only Cfh mRNA was detected in the retinal pigment epithelium, but no protein. PMID: 25684976
    10. A spectrum of complement dysregulation was modeled on the APOE4 age related macular degeneration mouse model by crossing these mice to complement factor H knockout (cfh-/-) mice to test the impact of excess complement activation. PMID: 24664701
    11. A2E accumulation altered retinal microglial complement regulation by decreasing complement factor H (and increasing complement factor B expression), favoring increased complement activation and lipofuscin deposition in the outer retina. PMID: 22819137
    12. endogenous fH makes a significant contribution to inhibition of the AP in CAIA through binding to sites of immune complex formation and complement activation. PMID: 23436934
    13. Properdin deficiency exacerbated renal injury in mice lacking complement factor H. PMID: 23184055
    14. nonsense mutations in SCR19 caused glomerulonephritis PMID: 23204401
    15. IL-27 regulates complement activation through up-regulation of complement factor H in the retina. PMID: 23029250
    16. CFH influences age-related macular degeneration risk by modulating oxidative stress, inflammation, and abnormal angiogenesis PMID: 22875704
    17. Although endogenous factor H (fH) limits the development of allergen-induced airway hyperresponsiveness and airway inflammation, the protection afforded by fH is incomplete. PMID: 22174452
    18. complement factor H (CFH) is a major malondialdehyde (MDA)-binding protein that can block both the uptake of MDA-modified proteins by macrophages and MDA-induced proinflammatory effects in vivo in mice PMID: 21979047
    19. though factor H doesn't fully prevent activation of the complement alternative pathway on ischemic tubules, its interaction with the tubule epithelial cell surface is critical for limiting complement activation and attenuating renal injury after ischemia. PMID: 21544060
    20. Complement factor H (CFH) mRNA is produced in CNS-specific cells, and in astrocytes, the CFH promoter is greatly influenced by the c-Jun and c-Fos transcription factors PMID: 21920606
    21. Deletion of adrenomedullin gene, but not of complement factor H, increases infarct volume after permanent focal cerebral ischemia in mice. PMID: 20854881
    22. Complement factor H deficiency accelerates development of lupus nephritis. PMID: 21148254
    23. Loss of complement factor H is associated with choroidal neovascularization. PMID: 20813971
    24. There is a significant reduction in the numbers of both neutrophils and macrophages in cardiac isografts isolated from mice treated with either complement receptor 2-Crry or CR2-factor H. PMID: 20962256
    25. Chimeric Cfh proteins led to (AMD)age-related macular degeneration-like characteristics in mice. This may represent a good model for studying the role of complement and other components of the immune system in early AMD. PMID: 20538999
    26. In the absence of plasma CFH, the accumulated activated complement leads to spontaneous and chronic serum sickness-induced proliferative glomerulonephritis. PMID: 20702729
    27. Complement factor H in the serum contributes to complement regulation on the apical surface of renal tubular epithelial cells. PMID: 20675597
    28. role for platelet-associated factor H to process immune complexes and limit their accumulation in glomeruli PMID: 15574507
    29. mFH and mFH1-5 do act as cofactors for factor I-mediated cleavage of human C3b. PMID: 16023208
    30. These data show that plasma Cfh limits complement activation in the circulation and other accessible sites such as the glomerulus, whereas platelet Cfh is responsible for immune complex processing. PMID: 16597679
    31. important role for C5 in both spontaneous membranoproliferative glomerulonephritis (MPGN) and experimentally induced nephritis in factor H-deficient mice and provide preliminary evidence that C5 inhibition therapy might be useful in human MPGN type II PMID: 16769899
    32. Alternative complement pathway activation during laser-induced choroidal neovascularization in complement-sufficient C57BL/6 mice is due to increased production of key component factor B as well as reduced expression of regulatory protein factor H. PMID: 16849499
    33. A significant level of CFH expression is maintained in different ocular tissues during development and aging. PMID: 16936129
    34. Mice intranasally infected with Streptococcus pneumoniae preincubated with Complement Factor H (FH) had increased bacteremia and lung invasion, but they had similar levels of nasopharyngeal colonization compared to mice challenged without FH. PMID: 17562771
    35. CFH is critically required for the long-term functional health of the retina. PMID: 17921253
    36. The data presented here provide significant new insight into the interaction between CspZ (BBH06)and Factor H and suggest that there is a correlation between CspZ production and dissemination. PMID: 19620346
    37. Complement receptor 2 with factor H specifically targets and inhibits the alternative pathway of complement in vitro and is effective in collagen antibody-induced arthritis in vivo. PMID: 19828624

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  • 亞細胞定位:
    Secreted.
  • 組織特異性:
    CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including monocytes, fibroblasts, or endothelial cells.
  • 數(shù)據(jù)庫鏈接:

    KEGG: mmu:12628

    STRING: 10090.ENSMUSP00000107607

    UniGene: Mm.8655



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