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  4. Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

  • 中文名稱：
    小鼠β淀粉樣蛋白1-40(Aβ1-40)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08300m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠β淀粉樣蛋白1-40(Aβ1-40)酶聯(lián)免疫試劑盒（CSB-E08300m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Aβ1-40含量。Aβ1-40是一種淀粉樣蛋白，是阿爾茨海默病（AD）病理過程中的關(guān)鍵分子。它是淀粉樣蛋白前體蛋白（APP）的代謝產(chǎn)物，通過β-折疊結(jié)構(gòu)聚集形成老年斑，對神經(jīng)細(xì)胞產(chǎn)生毒性，導(dǎo)致神經(jīng)元損傷和認(rèn)知功能下降。Aβ1-40的研究有助于揭示AD的發(fā)病機(jī)制，并為開發(fā)新的治療策略提供靶點(diǎn)。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，主要應(yīng)用于神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究，包括阿爾茨海默病小鼠模型建立、藥物干預(yù)后Aβ代謝通路評估、神經(jīng)毒性作用機(jī)制探索等方向，為病理機(jī)制解析和潛在治療策略開發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
  • 縮寫：
    Aβ1-40
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %80-97
    1:2Average %95
    Range %90-105
    1:4Average %95
    Range %83-100
    1:8Average %93
    Range %83-98
  • 回收率：
    The recovery of mouse Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-106
    EDTA plasma (n=4)8986-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.637 2.739 2.688 2.512
    10002.115 2.092 2.104 1.928
    5001.318 1.287 1.303 1.127
    2500.824 0.810 0.817 0.641
    1250.491 0.504 0.498 0.322
    62.50.322 0.315 0.319 0.143
    31.250.265 0.273 0.269 0.093
    00.172 0.180 0.176
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit, we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.


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