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中文名稱:小鼠脂聯素(ADP)酶聯免疫試劑盒
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貨號:CSB-E07272m
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規格:96T/48T
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價格:¥3800/¥2500
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其他:
產品詳情
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產品描述:小鼠脂聯素(ADP)酶聯免疫試劑盒(CSB-E07272m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的ADP含量。ADP即二磷酸腺苷,是生物體內能量代謝的關鍵物質。在細胞能量轉換過程中扮演重要角色。研究上,其與血小板活化相關,ADP結合受體可激活血小板信號通路,促進聚集。在藥物研發中,以ADP為靶點開發抗血小板藥物備受關注。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,靈敏度為0.039 ng/mL。該產品可廣泛應用于代謝相關疾病機制研究、藥物干預效果評估、體外細胞模型分泌功能分析以及動物實驗中的組織表達水平檢測,為脂聯素在能量代謝、炎癥反應及信號通路研究提供可靠工具,滿足科研領域對高靈敏度、寬線性范圍檢測的需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:Adipoq ELISA Kit; Acdc ELISA Kit; Acrp30 ELISA Kit; Apm1 ELISA Kit; Adiponectin ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; ACRP30 ELISA Kit; Adipocyte ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; Adipocyte-specific protein AdipoQ ELISA Kit
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縮寫:ADP
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
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檢測范圍:0.156 ng/ml-10 ng/ml.
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靈敏度:0.039 ng/ml
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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數據處理:
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貨期:3-5 working days
引用文獻
- Role of gut microbiota in Crohn's disease pathogenesis: Insights from fecal microbiota transplantation in mouse model Q Wu, LW Yuan, LC Yang, YW Zhang,World Journal of Gastroenterology,2024
- The Effect of 12 Weeks of Combined Training on the Levels of Adiponectin and Visfatin in Women with Gestational Diabetes A Mollanovruzi,/,2024
- Omega-3 fatty acids prevent gestational diabetes mellitus via modulating lipid metabolism X Zhang,researchsquare,2023
- Discovery of a potent allosteric activator of DGKQ that ameliorates obesity-induced insulin resistance via the sn-1, 2-DAG-PKCε signaling axis ZG Zheng,Cell metabolism,2022
- Egg Protein Transferrin-Derived Peptides Irw (Lle-Arg-Trp) and Iqw (Lle-Gln-Trp) Prevent Obesity Mouse Model Induced by a High-Fat Diet via Reducing Lipid Deposition and Reprogramming Gut Microbiota Z Liu,International Journal of Molecular Sciences,2022
- Aerobic exercise regulates FGF21 and NLRP3 inflammasome-mediated pyroptosis and inhibits atherosclerosis in mice XH Li,PloS one,2022
- IBF-R Regulates IRE1α Post-Translational Modifications and ER Stress in High-Fat Diet-Induced Obese Mice Hwa-Young Lee,Nutrients,2022
- Marein Regulates Insulin Resistance in Diabetic Nephropathy Mice by Inducing Autophagy Y Zhang,International Journal of Morphology,2021
- Marein Regula la Resistencia a la Insulina en Ratones con Nefropatía Diabética Induciendo la Autofagia T Li,International Journal of Morphology,2021
- The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus Y Yin,Pharmacological research,2021
- Lactococcus chungangensis CAU 28 alleviates diet-induced obesity and adipose tissue metabolism in vitro and in mice fed a high-fat diet Q Zhang,Journal of Dairy Science,2020
- Effects of Exogenous Adiponectin Supplementation in Early Pregnant PCOS Mice on the Metabolic Syndrome of Adult Female Offspring M Zuo,Research Square,2020
- Cajanolactone A, a stilbenoid from Cajanus cajan, prevents ovariectomy-induced obesity and liver steatosis in mice fed a regular diet ZH Luo,Phytomedicine,2020
- Theobromine alleviates diet-induced obesity in mice via phosphodiesterase-4 inhibition MH Jang,Eur J Nutr,2020
- Metformin Delays the Development of Atherosclerosis in Type 1 Diabetes Mellitus Via the Methylglyoxal Pathway A Liu,Diabetes Therapy,2020
- Hydrogen sulfide protects against high glucose Pan Z, et al,Molecular Medicine Reports,2019
- Leptin and Adiponectin Signaling Pathways Are Involved in the Antiobesity Effects of Peanut Skin Extract Xiang L, et al,Oxidative Medicine and Cellular Longevity,2019
- The role of adiponectin system in acute fasting-impaired mouse ovaries Yingying Han, et al,Reproduction,2019
- Protein-Bound β-glucan from Coriolus Versicolor has Potential for Use Against Obesity Li X, et al,Molecular Nutrition & Food Research,2019
- Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study Ahmed QU, et al,Biomolecules,2018
- Effects of diet zinc level on circadian rhythms and lipid metabolism in male mice Guan-ya Li.et al,biological rhythm research,2018
- Fungal polysaccharopeptides reduce obesity by richness of specific microbiota and modulation of lipid metabolism Xiaojun Li.et al,bioRxiv,2018
- Epigallocatechin-3-Gallate-Rich Green Tea Extract Ameliorates Fatty Liver and Weight Gain in Mice Fed a High Fat Diet by Activating the Sirtuin 1 and AMP Activating Protein Kinase Pathway Ui-Jin Bae.et al,Am. J. Chin. Med. ,2018
- Serine prevented high-fat diet-induced oxidative stress by activating AMPK and epigenetically modulating the expression of glutathione synthesis-related genes Zhou X.et al,Biochim Biophys Acta,2017
- Arachidonic acid sex-dependently affects obesity through linking gut microbiota-driven inflammation to hypothalamus-adipose-liver axis Zhuang P.et al,BBA-MOL BASIS DIS ,2017
- Leptin Gene Transfer Improves Symptoms of Type 2 Diabetic Mice by Regulating Leptin Signaling Pathway and Insulin Resistance of Peripheral Tissues Xiang L.et al,Hum Gene Ther.,2017
- Anti-Obesity Effects of the Mixture of Eriobotrya japonica and Nelumbo nucifera in Adipocytes and High-Fat Diet-Induced Obese Mice. Sharma BR.et al,Am J Chin Med. ,2017
- The beneficial effects of betaine on dysfunctional adipose tissue and N6-methyladenosine mRNA methylation requires the AMP-activated protein kinase a1 subunit Xihong Zhou. et al,The Journal of Nutritional Biochemistry,2015
- AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid Zhou X et al,Nutr Metab (Lond),2015
- CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and production of pathogenic IgG-antibodies Dennis Wolf et al,PLOS ONE,2012
產品評價
樣品類型:血清
樣品信息:小鼠
稀釋比:其他 1:2000
產品評價: 我用CSB-E07272m檢測高脂飲食誘導的C57小鼠,分別包括正常飲食、高脂誘導模型組及高脂誘導給藥組的小鼠血清,稀釋比例為1:2000;由于實驗室酶標儀較為老舊,稀釋倍數應在1:4000可能結果更好,同時標準曲線最高濃度10ng/ml與5ng/ml相近,故舍去最高濃度od值。
By 丁老師
相關產品
靶點詳情
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最新研究進展:脂聯素(adiponectin,ADP)是一種由脂肪組織分泌的激素,對于人體的葡萄糖代謝、脂質代謝、炎癥反應和心血管健康等生理過程具有至關重要的作用。最近的研究進展主要集中在以下幾個方面:ADP在代謝疾病中的作用備受關注。研究表明,ADP可以調節葡萄糖和脂質代謝,減少胰島素抵抗和脂肪肝的發生。此外,ADP還可以影響骨骼代謝和能量平衡,對代謝綜合征和肥胖相關疾病的發生和發展產生影響。ADP在炎癥反應中的作用也受到越來越多的關注。研究表明,ADP可以影響炎癥細胞的增殖和功能,影響炎癥介質的產生和釋放。此外,ADP還可以調節免疫細胞的活化和凋亡,對于炎癥反應和自身免疫性疾病具有調節作用。ADP在心血管健康中的作用也備受關注。研究表明,ADP可以降低血壓和血脂,預防動脈粥樣硬化和心血管疾病的發生。此外,ADP還可以調節心肌細胞的代謝和凋亡,保護心肌免受損傷和缺血再灌注損傷。
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功能:Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
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基因功能參考文獻:
- These data suggest that APN has a moderate regulatory role in oxidative stress-induced mitophagy and suppresses apoptosis. These findings demonstrate the antioxidant potential of APN in oxidative stress-associated skeletal muscle diseases. PMID: 28600493
- Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
- Study results indicate for the first time that adiponectin is able to influence the mechanical responses in strips from the mouse gastric fundus. PMID: 30254407
- miR-711, which is upregulated by Adipoq, represses TLR4 signaling, acting therefore as a major mediator of the anti-inflammatory action of Adipoq. PMID: 28240307
- both paracrine and endocrine effects of adiponectin may contribute to reduced reactive oxygen species generation and apoptosis after MI/R, in a CD36-dependent manner PMID: 29018142
- These data may indicate that insulin resistance in Adp(-/-) mice is likely caused by an increase in concentrations of TNFalpha and FFA via downregulation of PPARalpha. PMID: 29445073
- Adiponectin improves metabolic health but has only minor effects on reproductive functions in the polycystic ovary syndromemouse model. PMID: 28790184
- APN attenuates adverse cardiac remodeling following cardiac injury by up-regulating MMP-9 expression. APN up-regulates MMP-9 expression via activation of AMPK and ERK1/2. PMID: 29263115
- the effects of APN on the promotion of preadipocyte differentiation under inflammatory conditions may involve the PPARgamma signaling pathway, and at least partly depends on upregulation of PPARgamma expression. PMID: 29115433
- Results demonstrate that adiponectin enhances inhibitory postsynaptic current onto neuropeptide Y (NPY) neurons to attenuate action potential firing in NPY neurons in a glucose-independent manner, being contrasted to its glucose-dependent effect on proopiomelanocortin neurons. PMID: 28606559
- Results show a reciprocal regulation of adiponectin and FGF19 gene expression in mice. PMID: 27666676
- Acrp30 (a globular form of adiponectin) reduces the expression of proinflammatory cytokines and the expression of RAGE as beta amyloid transporters into brain. Moreover, Acrp30 attenuated the apoptosis and the tight junction disruption through AdipoR1-mediated NF-kappaB pathway in beta amyloid-exposed bEnd.3 cells. PMID: 29022894
- Findings demonstrate that adiponectin is an essential regulator of thermogenesis, and adiponectin is required for maintaining body temperature under cold exposure. PMID: 29058611
- Chronic stress accelerates DPP4-mediated GLP-1 degradation and alters plasma adiponectin, accelerating vascular senescence and impairing ischemia-induced neovascularization. PMID: 28963101
- AdipoQ antisense (AS) Long noncoding RNA (lncRNA) transfer from nucleus to cytoplasm inhibits adipogenesis through formation of an AdipoQ AS lncRNA/AdipoQ mRNA duplex to suppress the translation of AdipoQ mRNA.[ PMID: 29414510
- Adiponectin enhances quiescence exit of murine hematopoietic stem cells and hematopoietic recovery through mTORC1 potentiation. PMID: 28480607
- plasma adiponectin and leptin were also decreased in IL 10tm.These findings suggest that frailty observed in this mouse model of chronic inflammation may in part be driven by alterations in fat mass, hormone secretion and energy metabolism PMID: 29267271
- CpG ODNs decreased placental adiponectin expression in NOD mice and impaired human trophoblast function and was associated with increased embryo loss. Adiponectin may therefore play an important protective role in the prevention of bacteria-induced pregnancy failure. PMID: 27094728
- Caloric restriction (CR) impacted adiposity but only levels of the high molecular weight isoform of adiponectin responded to CR. PMID: 28156058
- T-cadherin was essential for accumulation of adiponectin in the neointima and atherosclerotic plaque lesions, and the adiponectin-T-cadherin association protected against vascular injury. PMID: 28062540
- mTORC1 mediated many of the beneficial actions of FGF21 in vitro, including UCP1 and FGF21 induction, increased adiponectin secretion, and enhanced glucose uptake without any adverse effects on insulin action. PMID: 27681418
- AnxA6 protein in adipocytes was upregulated by oxidative stress which might trigger AnxA6 induction in adipose tissues and contribute to impaired fat storage and adiponectin release. PMID: 27702590
- Adiponectin alters calcium and phosphate balance and renal mineral excretion, in part, through klotho. PMID: 27914707
- Adiponectin (ApN) proves to be a powerful protector of the skeletal muscle capable of reversing the disease progression, thus making it a potential therapeutic agent for Duchenne muscular dystrophy (DMD). PMID: 28463682
- These results demonstrated that LC along with insulin increases GSH levels thereby improving adiponectin secretion and glucose utilization in adipocytes. PMID: 28755973
- Adiponectin, TNF-alpha, and LOX-1 exert complex regulatory effects on the coronary microvascular endothelial function in atherosclerotic ApoE knockout mice. PMID: 27050429
- adiponectin inhibited endoplasmic reticulum stress and apoptosis of adipocyte in vivo and in vitro by activating the AMPK/PPARalpha/ATF2 pathway. PMID: 27882945
- Irisin improved endothelial function by modulating HO-1/ adiponectin axis in perivascular adipose tissue (PVAT) in HFD-induced obese mice. These findings suggest that regulating PVAT function may be a potential mechanism by which irisin improves endothelial function in obesity. PMID: 28595178
- a unique key feature of the T-cad prodomain is its involvement in binding of the T-cad repeats 1 and 2 to adiponectin; adiponectin positively regulates T-cad abundance PMID: 28325833
- adiponectin maintains intestinal homeostasis and protects against murine colitis through interactions with its receptor AdipoR1 and by modulating adaptive immunity and STAT3 signaling PMID: 28258220
- The KIF5B level was up-regulated during 3T3-L1 adipogenesis. This increase in cytosolic KIF5B was synchronized with the induction of adiponectin. Endogenous KIF5B and adiponectin were partially colocalized at the peri-nuclear and cytosolic regions. PMID: 27264953
- The elevation in circulating levels of adiponectin and Fgf15 led to normalized hepatic and serum levels of bile acids, limited hepatic accumulation of toxic bile, attenuated inflammation, and amelioration of liver injury in the ethanol-fed mNT knockout mice. PMID: 27573244
- Female adiponectin null mice displayed impaired fertility, reduced oocytes, disrupted estrous cycle, increased atretic follicles, and impaired late folliculogenesis. There was decrease in serum estradiol and FSH but an increase in LH and testosterone at proestrus. There was reduction of progesterone levels at diestrus, a significant decrease in LH receptor expression as well as in the number of GnRH immunoreactive neurons PMID: 27700136
- Tongqiaohuoxue decoction improved obesity-induced inflammation and insulin resistance by increasing adiponectin production. PMID: 27404230
- High salt is an important suppressor of cardioprotective APN and AdipoR1 in cardiac myocytes. PMID: 28051329
- PPARdelta activation in perirenal fat by agonist or high sodium intake inhibited renal sodium-glucose cotransporter 2 (SGLT2) function, which is mediated by increased production of adipose adiponectin. PMID: 27053360
- Acute knockdown of Insr or both Irs1 and Irs2 in adipocytes increased Adipoq mRNA expression but reduced adiponectin secretion. PMID: 26888756
- Adiponectin may protect the aorta from atherosclerotic injury by reducing inflammation. Adiponectin effectively inhibits the activation of NF-kappa B pathway by inhibiting the expression of NF-kappa B nuclear protein p65. PMID: 26965176
- Altered adiponectin multimerization could explain declined adiponectin levels and altered distribution of adiponectin complexes in the plasma of obese insulin-resistant individuals PMID: 26407855
- The expression of PI3K-insensitive GSK3 stimulates the production of adiponectin and protects from diet-induced metabolic syndrome. PMID: 27140617
- These data suggest that adiponectin could represent a possible biomarker in obesity-associated asthma PMID: 26462929
- ADPN might act as a biomarker of inflammation and have potential for the treatment of hemorrhagic shock. PMID: 26909947
- Adiponectin exerts novel effects to limit the production and action of mono-MPs, underscoring yet another pleiotropic effect of this adipokine. PMID: 26687997
- The results demonstrated a dynamic dysfunction of APN/AdipoR1 axis accompanying progression of diabetes mellitus in mice with cerebral ischemia. PMID: 26611106
- reduced plasma levels in obese mice treated with house dust mite allergens as compared to similarly treated lean mice PMID: 26476732
- CRP decreased adiponectin expression and multimerization, while CRP-induced decline in adiponectin might be mediated through the PI3K/Akt pathway. PMID: 26812237
- Globular Adiponectin (gAd) activates autophagy in myoblasts and gAd-activated autophagy drives the myogenic properties of this hormone. PMID: 26826036
- APN can help to reduce periodontitis-related bone loss, modulate JMJD3 and IRF4 expression, and macrophage infiltration. PMID: 26399931
- our data indicate that robust hypertrophic MEF2 activation in the heart in vivo requires a background of adiponectin signaling and that adiponectin signaling in primary isolated CM directly enhances MEF2 activity through activation of p38 MAPK PMID: 26196305
- In 3T3-L1 adipocytes, catechin and quercetin attenuated TNF-alpha-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. PMID: 25620282
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亞細胞定位:Secreted.
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組織特異性:Synthesized exclusively by adipocytes and secreted into plasma.
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數據庫鏈接:
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