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  4. Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

  • 中文名稱(chēng)：
    小鼠WAP四-二硫核心結(jié)構(gòu)域蛋白2(WFDC2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL026092MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠WAP四-二硫核心結(jié)構(gòu)域蛋白2(WFDC2)酶聯(lián)免疫試劑盒（CSB-EL026092MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的WFDC2含量。小鼠WAP四-二硫核心結(jié)構(gòu)域蛋白2(WFDC2)是一種分泌蛋白，含有兩個(gè)WAP結(jié)構(gòu)域，主要在男性生殖系統(tǒng)、呼吸道和鼻咽部表達(dá)。其在多種腫瘤細(xì)胞系中高表達(dá)，可能參與腫瘤的發(fā)生發(fā)展，并與支氣管擴(kuò)張有關(guān)，其研究機(jī)制涉及基因變異和蛋白質(zhì)功能。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，適用于基礎(chǔ)科研中探索WFDC2在疾病模型（如炎癥性疾病、纖維化病變或代謝紊亂）中的生物學(xué)功能，分析其在組織損傷修復(fù)中的動(dòng)態(tài)變化，或評(píng)估特定干預(yù)措施對(duì)WFDC2表達(dá)水平的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Wfdc2 ELISA Kit; He4 ELISA Kit; WAP four-disulfide core domain protein 2 ELISA Kit; WAP domain-containing protein HE4 ELISA Kit
  • 縮寫(xiě)：
    WFDC2
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WFDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 81-95  
    1:2 Average % 96  
    Range % 90-102  
    1:4 Average % 97  
    Range % 92-105  
    1:8 Average % 95  
    Range % 89-98  
  • 回收率：
    The recovery of mouse WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 92 86-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.045 2.144 2.095 1.943  
    2000 1.624 1.743 1.684 1.532  
    1000 1.053 1.072 1.063 0.911  
    500 0.673 0.663 0.668 0.516  
    250 0.342 0.351 0.347 0.195  
    125 0.274 0.283 0.279 0.127  
    62.5 0.229 0.235 0.232 0.080  
    0 0.152 0.151 0.152 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

I have a few questions on product CSB-EL026092MO ：
1. Which is this standard in this kit, natural material or recombinant?
If recombinant, please let us know expression method(E.coli etc.).
2. Is the standard or antibody, including any additive agent(antiseptic
agent or preserving agent)? If so, please let us know composition and
concentration.
3. Is this product including Stop solution? If so, please tell us
composition and concentration for stop solution.
4. Please let us know whether this kit include any hazardous
substances(azide, thimerosal, 2-ME, etc). If so, please let us know
composition and concentration.

A:
Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EL026092MO is NS0-derived recombinant protein
2,Preservative of this kit is proclin 300(1:3000)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.

靶點(diǎn)詳情

  • 功能：
    Broad range protease inhibitor.
  • 基因功能參考文獻(xiàn)：
    1. Causal testing implicates Wfdc2 and Hdac3 as novel genes affecting high density lipoprotein levels PMID: 21807117
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接：


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