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Mouse Vasoactive Intestinal Peptide,VIP ELISA Kit

  • 中文名稱:
    小鼠血管活性腸肽(VIP)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08356m
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠血管活性腸肽(VIP)酶聯(lián)免疫試劑盒(CSB-E08356m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的VIP含量。VIP即血管活性腸肽,是一種神經(jīng)遞質(zhì)和旁分泌激素。它在神經(jīng)調(diào)節(jié)、免疫調(diào)節(jié)、血管舒張等方面發(fā)揮作用。研究機(jī)制上,VIP可與特定受體結(jié)合,激活細(xì)胞內(nèi)信號(hào)通路,影響細(xì)胞的增殖、分化及炎癥反應(yīng)等,在多種疾病治療上具有潛在價(jià)值。試劑盒檢測(cè)范圍為7.8 pg/ml- 500 pg/ml,該產(chǎn)品適用于神經(jīng)內(nèi)分泌研究、消化系統(tǒng)功能探索、炎癥性疾病模型構(gòu)建等科研場(chǎng)景,例如通過檢測(cè)動(dòng)物模型中VIP的濃度變化,揭示其在腸道屏障調(diào)節(jié)或免疫微環(huán)境中的作用機(jī)制,為分子機(jī)理研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Vip ELISA Kit; VIP peptides [Cleaved into: Intestinal peptide PHI-42; Intestinal peptide PHI-27 ELISA Kit; Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide ELISA Kit; VIP ELISA Kit; Vasoactive intestinal polypeptide)] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    7.8 pg/ml - 500 pg/ml
  • 靈敏度:
    1.95 pg/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    88-96

    1:2

    Average %

    95

    Range %

    90-100

    1:4

    Average %

    98

    Range %

    92-104

    1:8

    Average %

    85

    Range %

    82-88

  • 回收率:

    The recovery of mouse VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    87-98

    EDTA plasma (n=4)

    93

    86-99

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.317

    2.356

    2.337

    2.177

    250

    1.579

    1.687

    1.633

    1.473

    125

    0.955

    0.997

    0.976

    0.816

    62.5

    0.586

    0.568

    0.577

    0.417

    31.2

    0.315

    0.326

    0.321

    0.161

    15.6

    0.250

    0.256

    0.253

    0.093

    7.8

    0.194

    0.197

    0.196

    0.036

    0

    0.154

    0.166

    0.160

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型:血清

樣品信息:小鼠

稀釋比:沒有稀釋

產(chǎn)品評(píng)價(jià): 我用CSB-E08356m檢測(cè)正常血清,其od值為1.831/1.678/1.534/1.702/1.945/1.609可以正常檢測(cè)

By 張老師

靶點(diǎn)詳情

  • 功能:
    VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.; PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin. PHM also causes vasodilation.
  • 基因功能參考文獻(xiàn):
    1. A significant myenteric neuronal cell loss and upregulation of VIP expression were found after focal, but not global, cerebral ischaemia. PMID: 29577471
    2. Martinotti cells in layers II/III of the mouse primary somatosensory cortex are inhibited by both parvalbumin (PV)- and vasoactive intestinal polypeptide (VIP)-expressing cells. PMID: 27897179
    3. BAD phosphorylation is essential in the cytoprotective effect of vasoactive intestinal peptide on cancer stem cells. PMID: 28569785
    4. The suprachiasmatic nucleus (SCN)network consists of multiple clusters of cellular circadian rhythms that are differentially integrated by AVP and VIP signaling, depending on the postnatal period PMID: 27626074
    5. PACAP/PAC1 signaling is important for light regulated behavior, VIP/VPAC2 signaling for stable clock function and both signaling pathways may play a role in shaping diurnality versus nocturnality. PMID: 29155851
    6. The data of this study support a key role for VIP interneurons in cortical circuit development and suggest a possible contribution to pathophysiology in neurodevelopmental disorders PMID: 28817803
    7. During arousal, VIP cells rapidly and directly inhibit pyramidal neurons; VIP cells also indirectly excite these pyramidal neurons via parallel disinhibition. PMID: 28114295
    8. This study found that VIP-/- mice show a bilateral tactile hypersensitivity after spared nerve injury and a bilateral increase in neuronal activation upon innocuous tactile paw stimulation. PMID: 28359740
    9. we found that VIP inhibits NFAT nuclear translocation in primary human pulmonary artery smooth muscle cells (PASMC). Early activation of NFATc3 in IPF patients may contribute to disease progression and the increase in VIP expression could be a protective compensatory mechanism PMID: 28125639
    10. Several studies have shown that VIP(+) cells are sensitive to neuromodulation and increase their firing during locomotion, whisking, and pupil dilation and are involved in spatially specific top-down modulation, reminiscent of the effects of top-down attention, and also that attention enhances spatial resolution. Our findings provide a bridge between these studies by establishing the inhibitory circuitry that regulates th PMID: 27911754
    11. majority of GnRH neurons in male and female mice express functional VIP receptors; effects of VIP on GnRH neurons do not alter across the estrous cycle PMID: 27501185
    12. Backup mechanisms maintain PACAP/VIP-induced arterial vasodilation in PACAP-deficient mice. PMID: 28490016
    13. in cortical VIP neurons, experience-dependent gene transcription regulates visual acuity by activating the expression of IGF1, thus promoting the inhibition of disinhibitory neurons and affecting inhibition onto cortical pyramidal neurons PMID: 26958833
    14. Chronic loss of VIP in mice leads to a disruption of certain but not all immunological compartments. PMID: 25301381
    15. neuropeptides CGRP and VIP have an important role in suppressing bone resorptive activities through RANKL/OPG pathway, similar to mechanical loading. PMID: 24717410
    16. The results show in vivo a primary role for VIP chronic exposure in CFTR membrane stability and function and confirm in vitro data. PMID: 24898584
    17. VIP signaling modulates the output from the olfactory bulb to maintain circadian rhythms in the mammalian olfactory system. PMID: 24760863
    18. VIP neuropeptide-mediated cAMP-PKA signaling has an important role in hepatic homeostasis and cytoprotection in vivo PMID: 23744729
    19. Loss of the VIP gene orchestrates a panoply of pathogenic genes which are detrimental to both left and right cardiac homeostasis. PMID: 23700405
    20. Vasoactive intestinal peptide may be an essential factor for Hertwig's epithelial root sheath development. PMID: 22925217
    21. With cyclophosphamide-induced cystitis,VIP transcript expression significantly increased in the urothelium (48 h) of NGF-OE mice. PMID: 22700375
    22. Peritoneal cells isolated from VIP KO naive mice produced lower levels of proinflammatory cytokines in response to LPS in vitro. PMID: 22615845
    23. The data indicate that the intact circadian system can compensate for some of the consequences of the loss of VIP, whereas this peptide is indispensable for endogenous encoding of seasonal information. PMID: 22512278
    24. Stimulation with VIP significantly increased cyclic AMP formation in colon preparations from control but not DSS-treated mice. PMID: 21939768
    25. Data suggest that the Vip gene is not required for induction of a gene expression program linked to small bowel growth after enhancement of GLP-2 receptor signaling. PMID: 22535770
    26. VIP increased the presence of immunoregulatory cytokines. VIP also modulates Th2/Th9 and iTreg/Th1 ratios. PMID: 21445087
    27. PACAP and VIP are endogenous mediators that likely regulate immunity and immune-mediated diseases within the skin. PMID: 22531916
    28. These data support the concept that VIP influences the endogenous oxidant/antioxidant balance. One potential implication is that VIP and its analogues may be used to treat inflammatory diseases, including asthma. PMID: 22103391
    29. VIP and pituitary adenylate cyclase-activating polypeptide 38 (PACAP-38), but not secretin, stimulated rankl mRNA expression in mouse calvarial osteoblasts. VIP inhibited mRNA expressions of opg and m-csf, effects shared by PACAP-38, but not by secretin PMID: 21815197
    30. results support that decline in VIP/VPAC local levels may influence survival/apoptosis intracellular set point in NOD acinar cells and their clearance, contributing to gland homeostasis loss in a model of Sjogren's syndrome PMID: 22059987
    31. The reduced birth rate at 16-week-old NOD mice with a Th1 systemic cytokine profile involves resorption processes with a lower expression of VIP at the sites of implantation, which acts as a local inducer of pro-implantatory LIF and Treg activation. PMID: 19633131
    32. VIP blockade induces microcephaly through Mcph1 signaling; VIP/Mcph1/Chk1 signaling is key for normal cortical development PMID: 21737879
    33. absence of this peptide alters both the amplitude of circadian rhythms as well as the phase relationships between the rhythms in the suprachiasmatic nucleus and periphery PMID: 21628547
    34. Antigen-specific primary and secondary immune responses are accelerated in VIP-deficient (KO) mice and in mice reconstituted with VIP-KO hematopoietic cells, supporting the role of VIP in immune counter-regulatory pathways. PMID: 21677142
    35. the VIP/VPAC2 system induces reactive astrocytosis and plays a key role in neuroprotection against excitotoxicity in neurological disorders PMID: 21281617
    36. VIP enhancement of the severity of dextran sodium sulfate-induced colitis is mediated solely by VPAC1 receptors in mice. PMID: 21295288
    37. The results suggest that VIP has a role in the neural control of pelvic organ function and activation of VPAC and/or PACAP receptors can modulate the activity of the autonomic neurons innervating pelvic organs. PMID: 20428965
    38. Data describe PACAP, vasoactive intestinal polypeptide, and PAC1, VPAC1, VPAC2 transcripts or protein expression in urothelium and detrusor smooth muscle and lumbosacral dorsal root ganglia in NGF-overexpressing and wildtype mice. PMID: 20449688
    39. VIP provides significant protection against spontaneous diabetes by modulating T-cell subsets and counterbalancing tolerance and immunity. PMID: 20309012
    40. PACAP/VIP as well as their three classes of receptors are important in many physiological/pathophysiological processes, some of which are identified in these studies using knockout animals. PMID: 21157320
    41. These results demonstrate that VIP is an important regulator of physiological circadian rhythmicity in the heart. PMID: 20952671
    42. The study provides new insights into the local regulation of VIP in the columnar epithelia of the allergic lung. PMID: 20566347
    43. VIP plays an unanticipated permissive and/or proinflammatory role in the propagation of the inflammatory response in the CNS PMID: 20978211
    44. VIP and PACAP regulate the responsiveness of cells within the suprachiasmatic nucleus to the effects of light PMID: 20180841
    45. VIP knockout mice demonstrate elevated plasma glucose, insulin, and leptin levels, with no islet beta-cell number/topography alteration. PMID: 20150284
    46. These results confirm that TH2, but not TH1 cells, express and secrete functional VIP following specific antigen stimulation. PMID: 11935374
    47. VIP and PACAP have an anti-inflammatory role by a new mechanism associated with impairment of a key component of the chemokine network PMID: 12090758
    48. There was a deficient VIP-activated signaling associated with a reduced saliva and amylase secretion in response to VIP, suggesting impaired balance of neuroimmune interactions in reduced salivary secretion of NOD mice. PMID: 12225893
    49. VIP/PACAP interference with the p65/CBP interaction in activated microglia may represent a significant element in the regulation of the inflammatory response in the CNS by the endogenous neuropeptides PMID: 12372411
    50. This peptide regulates nerve growth factor in the embryonic mouse. PMID: 12383868

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  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Glucagon family
  • 數(shù)據(jù)庫鏈接:


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