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Mouse Troponin T, cardiac muscle(TNNT2) ELISA kit

  • 中文名稱(chēng):
    小鼠心肌特異性肌鈣蛋白T(TNNT2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL024016MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠心肌特異性肌鈣蛋白T(TNNT2)酶聯(lián)免疫試劑盒(CSB-EL024016MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的TNNT2含量。TNNT2是一個(gè)重要靶點(diǎn)。它是心肌肌鈣蛋白T的編碼基因,與心肌收縮等生理功能密切相關(guān)。在研究機(jī)制方面,其異常表達(dá)或突變可影響心肌功能,引發(fā)多種心臟疾病,目前針對(duì)該靶點(diǎn)在探索診斷標(biāo)志物及治療藥物等。試劑盒檢測(cè)范圍為31.25 pg/ml-2000pg/ml,適用于科研領(lǐng)域中心肌損傷相關(guān)機(jī)制研究;適用于評(píng)估小鼠心肌損傷模型中的TNNT2動(dòng)態(tài)變化,為心血管疾病機(jī)制探索、藥物心臟毒性評(píng)價(jià)及轉(zhuǎn)基因動(dòng)物模型表型分析提供可靠工具,尤其適用于心肌梗死、心肌肥厚等病理過(guò)程中生物標(biāo)志物的定量研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    31.25 pg/ml-2000pg/ml
  • 靈敏度:
    7.8 pg/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線(xiàn)性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    94

    Range %

    89-102

    1:2

    Average %

    91

    Range %

    82-98

    1:4

    Average %

    93

    Range %

    85-100

    1:8

    Average %

    92

    Range %

    88-100

  • 回收率:

    The recovery of mouse TNNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    90-100

    EDTA plasma (n=4)

    92

    87-96

  • 標(biāo)準(zhǔn)曲線(xiàn):

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    1.912

    1.927

    1.920

    1.749

    1000

    1.513

    1.569

    1.541

    1.370

    500

    1.075

    1.138

    1.107

    0.936

    250

    0.804

    0.798

    0.801

    0.630

    125

    0.511

    0.537

    0.524

    0.353

    62.5

    0.349

    0.356

    0.353

    0.182

    31.25

    0.254

    0.245

    0.250

    0.079

    0

    0.172

    0.169

    0.171

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能參考文獻(xiàn):
    1. Cardiac troponin T at the NMJ region contributes to NMJ functional decline with ageing mainly in the fast-twitch skeletal muscle through interfering with PKA signalling. PMID: 28419739
    2. Hypertrophic cardiomyopathy pathophysiology is "mutation specific" in mice models carrying TNNT2 E163R and R92Q mutations. PMID: 28735292
    3. TnTA30V mutation attenuated Ca(2+)-activated maximal tension and length-mediated cross-bridge recruitment against alpha-myosin heavy chain but augmented these parameters against beta-myosin heavy chain, suggesting divergent contractile phenotypes. PMID: 27769999
    4. The shift from cTnT exon 5 inclusion to exclusion during development was delayed in the heart of Ts65Dn mice due to Dyrk1A overexpression. PMID: 27049307
    5. for hypertrophic cardiomyopathy (HCM)-causing mutations in TnT, Ca(2+)-sensitisation together with uncoupling in vitro is the usual response and both factors may contribute to the HCM phenotype PMID: 27036851
    6. cardiomyopathy mutation (R97L) in mouse cardiac troponin T has an effect on the muscle length-mediated recruitment of crossbridges and is modified divergently by alpha- and beta-myosin heavy chain PMID: 26792537
    7. Data indicate that high-sensitivity troponin T (hs-TnT) levels are influenced by myocardial dysfunction/heart failure (HF) in acute exacerbation of chronic obstructive lung disease (AECOPD), but provide independent prognostic information. PMID: 26754170
    8. cTnT elevation emerged as a strong, independent predictor of 30-day mortality and remained a modest, but significant, predictor throughout 2 years post transcatheter aortic valve implantation. PMID: 26896474
    9. MBPC and troponin-I phosphorylation modulate myofilament length-dependent activation PMID: 26453301
    10. Data indicate that the troponin T Tnnt2(MerCreMer/+) mouse model also provides a useful tool to trace myocardial lineage during development. PMID: 26010701
    11. TnT mutation F72L leads to contractile changes that are linked to dilated cardiomyopathy in the presence of MYH6 and hypertrophic cardiomyopathy in the presence of MYH7. PMID: 26342069
    12. TNT increases slightly during low flux-hemodialysis. High-flux hemodialysis eliminates the biomarker and can mask increases caused by cardiac disease. PMID: 25744196
    13. Mice carrying the Delta160E or E163R mutations exhibit sarcomeric disruption. PMID: 24480310
    14. Cardiac muscle activation blunted by a mutation to the regulatory component, troponin T. PMID: 23897817
    15. Study is the first to show that the interplay between the N terminus of cTnT and the overlapping ends of contiguous Tm effectuates different states of Tm on the actin filament. PMID: 23748972
    16. We conclude that the observed clinical severity of the cTnT Delta160E mutation is caused by a combination of direct sarcomeric disruption coupled to a profound dysregulation of Ca(2+) homeostasis PMID: 23434821
    17. Report two distinct functional regions within the N-terminus of cTnT with divergent roles in mediating cardiac contractile activation. PMID: 23207592
    18. findings provide new evidence to demonstrate that R92 mutation effects on cardiac contractile function and dynamics are influenced by myosin heavy chain isoform PMID: 22884844
    19. role of N-terminal extension of cardiac troponin T in cardiac thin filament PMID: 23009843
    20. results showed dominantly negative effects of the coexistence of fast skeletal muscle TnT and cardiac TnT on contractile kinetics absent the influence of extracellular matrix; a dilatative remodeling at the cellular level was observed, supporting a pathological significance PMID: 22538236
    21. Data sugget that knockdown or downregulation of CYP2E1 might be a therapeutic strategy to control the development of dilated cardiomyopathy (DCM) after mutations of cTnT(R141W) or other factors. PMID: 22665122
    22. Correlation of molecular and functional effects of mutations in cardiac troponin T linked to familial hypertrophic cardiomyopathy PMID: 22334656
    23. Pak1-knockout hearts have reduced recovery of myocardial performance after global I/R injury concomitant with changes in troponin-T and MLC2 phosphorylation and protein association. PMID: 22037191
    24. One major and two minor alternatively spliced isoforms of troponin T have been purified from wild-type healthy adult mouse heart. PMID: 21639091
    25. TCDD represses the expression of the cardiac development-specific Nkx2.5 homeobox transcription factor, of cardiac troponin-T and of alpha- and beta-myosin heavy chains. PMID: 20130022
    26. Results indicated a pathogenic mechanism in which the coexistence of functionally different cTnT variants in cardiac muscle reduces myocardial efficiency due to desynchronized thin filament activation. PMID: 20418479
    27. Cardiomyopathy-causing deletion K210 in cardiac troponin T alters phosphorylation propensity of sarcomeric proteins. PMID: 20079745
    28. Studies show that the rat Tnnt2-rtTA;TetO-Cre mice transgenic line a valuable genetic tool for analysis of spatiotemporal gene function and cardiomyocyte lineage tracing during developmental and postnatal period. PMID: 20014345
    29. Identification of a functionally critical protein kinase C phosphorylation residue PMID: 12832403
    30. Transgenic mouse models expressing a missense mutation (R92Q) or a splice donor site mutation (trunc) in the cardiac troponin T (cTnT) showed that hypertrophic signaling is differentially affected by distinct mutations in cTnT. PMID: 15466629
    31. Changes in thin filament structure caused by single amino acid substitutions lead to differences in the biophysical properties of cTnT and alter cardiomyopathy pathogenesis. PMID: 16326803
    32. The mu-calpain-mediated proteolytic modification of TnT may act as an acute mechanism to adjust muscle contractility under stress conditions. PMID: 16981728
    33. the hypervariable NH2-terminal region modulates the conformation and function of the TnT core structure to fine-tune muscle contractility. PMID: 17260966
    34. Independent FHC-relted Tnt mutations exhibit specific alterations in myocellular and calcium kinetics. PMID: 17490679
    35. R-92L and R-92W mutations in the TM-binding domain of cardiac TnT alter thin filament structure and flexibility sufficiently to cause severe defects in both whole heart energetics and contractile performance PMID: 17526570
    36. Transgenic mice expressing chicken TNNT3 and cTNT in myocardium showed ventricular dysfunction and chronic myocardial hypertrophy and degeneration. PMID: 17959729
    37. Plasma troponin T was at a similar level in beta1(-/-), beta1(+/+) and beta1(-/-) BM mice treated with 30 min ischaemia and 3 h reperfusion. PMID: 18296493
    38. Differential interactions among the sarcomeric proteins containing cTnT-Q92 or cTnT-W141 are responsible for the contrasting phenotypes of hypertrophic cardiomyopathy or dilated cardiomyopathy, respectively. PMID: 18349139
    39. Data found that fTnT normally associated with fast twitch skeletal muscle were present at significant levels in the thoracic aorta, and that fTnT transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes PMID: 18548613
    40. Absence of one Tnnt2 allele leads to a mild deficit in transcript but not protein, leading to a normal cardiac phenotype. PMID: 18612386
    41. These findings indicate cTnT plays a critical role in sarcomere assembly during myofibrillogenesis in the embryonic heart, and that the membrane excitation and intracellular Ca(2+) handling systems develop independently of the contractile system. PMID: 18671960
    42. The direct repeat and overlapping Troponin T GATA site are critical for the expression level and cardiac specificity. PMID: 18951515
    43. Data indicate that UT-B deletion caused the dynamic expression regulation of TNNT2 and ANP, and these proteins may provide new clues to investigate the molecular events involved in cardiac conduction. PMID: 19132680
    44. peak releases of TnT or TnT fragments from decomposition of a large number of myofibrils in acute myocardial infarction may breach the cellular protection of proteolytic degradation PMID: 19395545
    45. cTnT mutations in the TNT1 domain result in mutation-specific effects and a different temporal onset of altered myocellular mechanics, Ca(2+) kinetics, and Ca(2+) homeostasis; may contribute to clinical variability in familial hypertrophic cardiomyopathy PMID: 19502551
    46. Results describe the functional effects of N-terminal deletion and phosphorylation in cardiac troponin T on Ca(2+) dependence of myofilament isometric force production, isometric ATPase rate, and thin filament sliding speed. PMID: 19586048

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  • 蛋白家族:
    Troponin T family
  • 數(shù)據(jù)庫(kù)鏈接:


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