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  4. Mouse Transforming Growth factor β3,TGF-β3 ELISA kit

Mouse Transforming Growth factor β3,TGF-β3 ELISA kit

  • 中文名稱：
    小鼠轉化生長因子β3(TGF-β3)酶聯免疫試劑盒
  • 貨號：
    CSB-E12862m
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠轉化生長因子β3(TGF-β3)酶聯免疫試劑盒（CSB-E12862m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TGFB3含量。TGFB3即轉化生長因子β3，在胚胎發育、組織修復等多種生理過程中扮演重要角色。其研究機制主要圍繞調節細胞生長、分化、黏附及凋亡等方面。異常表達與腫瘤發生發展、纖維化疾病等密切相關，對其深入研究有望為相關疾病治療提供新策略。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，本試劑盒適用于體外實驗研究，可廣泛用于探究TGF-β3在細胞功能調控、炎癥或纖維化動物模型中的動態變化，亦可用于評估體外培養細胞分泌TGF-β3的能力，為研究組織再生、疾病機制及藥物干預效果提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Tgfb3 ELISA Kit; Transforming growth factor beta-3 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-3 ELISA Kit; TGF-beta-3)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %95
    Range %92-102
    1:4Average %96
    Range %93-102
    1:8Average %94
    Range %89-99
  • 回收率：
    The recovery of mouse TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-99
    EDTA plasma (n=4)9184-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.646 2.815 2.731 2.542
    40002.016 2.155 2.086 1.897
    20001.195 1.264 1.230 1.041
    10000.778 0.798 0.788 0.599
    5000.493 0.506 0.500 0.311
    2500.336 0.361 0.349 0.160
    1250.279 0.265 0.272 0.083
    00.192 0.185 0.189 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.; Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.; Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
  • 亞細胞定位：
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-3]: Secreted.
  • 蛋白家族：
    TGF-beta family
  • 數據庫鏈接：


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