Mouse Thrombopoietin,TPO ELISA kit
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中文名稱:小鼠血小板生成素(TPO)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04746m-IS
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠血小板生成素(TPO)酶聯(lián)免疫試劑盒(CSB-E04746m-IS)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的THPO含量。THPO即血小板生成素,其背景在于它能特異性調(diào)節(jié)血小板生成。研究機(jī)制上,它與造血干細(xì)胞、巨核系祖細(xì)胞表面受體結(jié)合,促進(jìn)其增殖、分化和成熟,還能增加巨核細(xì)胞的數(shù)量及體積,從而使血小板生成增多,維持血小板平衡。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL,靈敏度為15.6 pg/mL。本試劑盒適用于小鼠模型中與血小板生成、再生障礙性貧血、骨髓功能異常相關(guān)的機(jī)制研究,或用于評(píng)估藥物干預(yù)、基因修飾對(duì)TPO調(diào)控通路的影響,為血液學(xué)、免疫學(xué)及病理生理學(xué)領(lǐng)域的科研實(shí)驗(yàn)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:Thpo ELISA Kit; Thrombopoietin ELISA Kit; C-mpl ligand ELISA Kit; ML ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; MGDF ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:15.6 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
93
Range %
86-100
1:2
Average %
97
Range %
91-103
1:4
Average %
87
Range %
82-95
1:8
Average %
91
Range %
86-98
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回收率:
The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
86-101
EDTA plasma (n=4)
98
92-105
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2000
3.261
3.114
3.187
3.064
1000
2.533
2.427
2.480
2.357
500
1.536
1.528
1.532
1.409
250
0.916
0.939
0.928
0.805
125
0.575
0.591
0.583
0.460
62.5
0.387
0.373
0.380
0.257
31.25
0.240
0.234
0.237
0.114
0
0.121
0.125
0.123
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數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
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基因功能參考文獻(xiàn):
- Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively... IL-11 promoted Hematopoietic stem cell engraftment in a mouse model of aplastic anemia an effect that was attenuated in cells overexpressing miR-204-5p. PMID: 29217821
- this study assessed the physiological source of thrombopoietin, a key cytokine required for maintaining hematopoietic stem cell . PMID: 29622652
- TPO and its receptor Mpl are dispensable for platelet survival in adult mice PMID: 27344013
- Furthermore, although the contribution of the TPO treated graft to long-term hematological engraftment was reduced, the TPO treated and untreated grafts both contributed significantly to long-term chimerism in vivo. PMID: 25137252
- TPO treatment also promoted the peripheral induction of Foxp3(+) Tregs in conjunction with elevated circulating TGF-beta levels. PMID: 25212676
- novel findings about aspects of TPO action on stem cells PMID: 25564715
- Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
- Thrombopoietin/MPL signaling confers growth and survival capacity to CD41-positive cells in a mouse model of Evi1 leukemia. PMID: 25298035
- Megakaryocytes regulate cell cycle quiescence of hematopoietic stem cell through the production of thrombopoietin. PMID: 25451253
- IEX-1 has a role in activation of Erk and NF-kB pathways, which affects thrombopoietin-promoted NHEJ DNA repair in hematopoietic stem cells PMID: 24184684
- Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID: 22284746
- Signal transduction pathway of ERK1/2 participates in the activation of thrombopoietin in inflammatory injury of BV2 cells. PMID: 21507308
- The implication of Tpo as a mediator of neuronal damage in experimental pneumococcal meningitis is reported. PMID: 21149592
- Administration of recombinant human IL11 after supralethal radiation exposure promotes survival in mice: interactive effect with thrombopoietin PMID: 12005542
- glycan domain of thrombopoietin acts in trans to enhance secretion of the hormone and other cytokines PMID: 12101178
- Thrombopoietin expands hematopoietic stem cells after transplantation PMID: 12163458
- p38 mitogen-activated protein kinase (MAPK) was responsible for the TPO-induced Hoxb4 elevation PMID: 12855555
- Mpl stimulation by TPO results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID: 14726379
- Thrombopoietin induces HOXA9 nuclear transport in immature hematopoietic cells PMID: 15254242
- mpl-tpo signaling pathways are negatively modulated by lnk PMID: 15337790
- is strongly proapoptotic in the brain, causing death of newly generated neurons through the Ras-extracellular signal-regulated kinase 1/2 pathway PMID: 15642952
- TPO(high) and GATA-1(low) alterations are linked in an upstream-downstream relationship along a pathobiologic pathway leading to development of myelofibrosis PMID: 15665119
- Development of myelofibrosis and osteosclerosis depends on local TPO levels in Bone marrow cells. PMID: 15927672
- Thrombopoietin synthesis is induced by IL-6 in the liver during acute inflammation. PMID: 16022585
- results suggest that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in HSCs PMID: 17284614
- thrombopoietin has a role in mast cell differentiation [review] PMID: 17468237
- Chronic TPO overexpression induces mesangioproliferative glomerulopathy. PMID: 17546634
- roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit. PMID: 17925492
- Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.( PMID: 18410987
- TPO induces HIF-1alpha expression in a manner very similar to that of hypoxia. PMID: 18473128
- thrombopoietin potentiates vasculogenesis by enhancing motility and enlivenment of transplanted endothelial progenitor cells via activation of Akt/mTOR/p70S6kinase signaling pathway PMID: 18773906
- TPO downmodulates c-Myb expression through induction of miR-150. PMID: 18814950
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亞細(xì)胞定位:Secreted.
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蛋白家族:EPO/TPO family
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組織特異性:Found mainly in the liver, kidney and skeletal muscle.
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