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Mouse Thrombopoietin,TPO ELISA kit

  • 中文名稱:
    小鼠血小板生成素(TPO)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E04746m-IS
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠血小板生成素(TPO)酶聯(lián)免疫試劑盒(CSB-E04746m-IS)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的THPO含量。THPO即血小板生成素,其背景在于它能特異性調(diào)節(jié)血小板生成。研究機(jī)制上,它與造血干細(xì)胞、巨核系祖細(xì)胞表面受體結(jié)合,促進(jìn)其增殖、分化和成熟,還能增加巨核細(xì)胞的數(shù)量及體積,從而使血小板生成增多,維持血小板平衡。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL,靈敏度為15.6 pg/mL。本試劑盒適用于小鼠模型中與血小板生成、再生障礙性貧血、骨髓功能異常相關(guān)的機(jī)制研究,或用于評(píng)估藥物干預(yù)、基因修飾對(duì)TPO調(diào)控通路的影響,為血液學(xué)、免疫學(xué)及病理生理學(xué)領(lǐng)域的科研實(shí)驗(yàn)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Thpo ELISA Kit; Thrombopoietin ELISA Kit; C-mpl ligand ELISA Kit; ML ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; MGDF ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    86-100

    1:2

    Average %

    97

    Range %

    91-103

    1:4

    Average %

    87

    Range %

    82-95

    1:8

    Average %

    91

    Range %

    86-98

  • 回收率:

    The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    86-101

    EDTA plasma (n=4)

    98

    92-105

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    3.261

    3.114

    3.187

    3.064

    1000

    2.533

    2.427

    2.480

    2.357

    500

    1.536

    1.528

    1.532

    1.409

    250

    0.916

    0.939

    0.928

    0.805

    125

    0.575

    0.591

    0.583

    0.460

    62.5

    0.387

    0.373

    0.380

    0.257

    31.25

    0.240

    0.234

    0.237

    0.114

    0

    0.121

    0.125

    0.123

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • 基因功能參考文獻(xiàn):
    1. Increased TPO expression in HSCs following IL-11 exposure could be mimicked or blocked by inhibiting or overexpressing miR-204-5p, respectively... IL-11 promoted Hematopoietic stem cell engraftment in a mouse model of aplastic anemia an effect that was attenuated in cells overexpressing miR-204-5p. PMID: 29217821
    2. this study assessed the physiological source of thrombopoietin, a key cytokine required for maintaining hematopoietic stem cell . PMID: 29622652
    3. TPO and its receptor Mpl are dispensable for platelet survival in adult mice PMID: 27344013
    4. Furthermore, although the contribution of the TPO treated graft to long-term hematological engraftment was reduced, the TPO treated and untreated grafts both contributed significantly to long-term chimerism in vivo. PMID: 25137252
    5. TPO treatment also promoted the peripheral induction of Foxp3(+) Tregs in conjunction with elevated circulating TGF-beta levels. PMID: 25212676
    6. novel findings about aspects of TPO action on stem cells PMID: 25564715
    7. Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
    8. Thrombopoietin/MPL signaling confers growth and survival capacity to CD41-positive cells in a mouse model of Evi1 leukemia. PMID: 25298035
    9. Megakaryocytes regulate cell cycle quiescence of hematopoietic stem cell through the production of thrombopoietin. PMID: 25451253
    10. IEX-1 has a role in activation of Erk and NF-kB pathways, which affects thrombopoietin-promoted NHEJ DNA repair in hematopoietic stem cells PMID: 24184684
    11. Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID: 22284746
    12. Signal transduction pathway of ERK1/2 participates in the activation of thrombopoietin in inflammatory injury of BV2 cells. PMID: 21507308
    13. The implication of Tpo as a mediator of neuronal damage in experimental pneumococcal meningitis is reported. PMID: 21149592
    14. Administration of recombinant human IL11 after supralethal radiation exposure promotes survival in mice: interactive effect with thrombopoietin PMID: 12005542
    15. glycan domain of thrombopoietin acts in trans to enhance secretion of the hormone and other cytokines PMID: 12101178
    16. Thrombopoietin expands hematopoietic stem cells after transplantation PMID: 12163458
    17. p38 mitogen-activated protein kinase (MAPK) was responsible for the TPO-induced Hoxb4 elevation PMID: 12855555
    18. Mpl stimulation by TPO results in the activation of Lyn kinase, which appears to limit the proliferative response through a signaling cascade that regulates MAPK activity PMID: 14726379
    19. Thrombopoietin induces HOXA9 nuclear transport in immature hematopoietic cells PMID: 15254242
    20. mpl-tpo signaling pathways are negatively modulated by lnk PMID: 15337790
    21. is strongly proapoptotic in the brain, causing death of newly generated neurons through the Ras-extracellular signal-regulated kinase 1/2 pathway PMID: 15642952
    22. TPO(high) and GATA-1(low) alterations are linked in an upstream-downstream relationship along a pathobiologic pathway leading to development of myelofibrosis PMID: 15665119
    23. Development of myelofibrosis and osteosclerosis depends on local TPO levels in Bone marrow cells. PMID: 15927672
    24. Thrombopoietin synthesis is induced by IL-6 in the liver during acute inflammation. PMID: 16022585
    25. results suggest that a balance in positive and negative signals downstream from the TPO signal plays a role in the regulation of the probability of self-renewal in HSCs PMID: 17284614
    26. thrombopoietin has a role in mast cell differentiation [review] PMID: 17468237
    27. Chronic TPO overexpression induces mesangioproliferative glomerulopathy. PMID: 17546634
    28. roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit. PMID: 17925492
    29. Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.( PMID: 18410987
    30. TPO induces HIF-1alpha expression in a manner very similar to that of hypoxia. PMID: 18473128
    31. thrombopoietin potentiates vasculogenesis by enhancing motility and enlivenment of transplanted endothelial progenitor cells via activation of Akt/mTOR/p70S6kinase signaling pathway PMID: 18773906
    32. TPO downmodulates c-Myb expression through induction of miR-150. PMID: 18814950

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  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    EPO/TPO family
  • 組織特異性:
    Found mainly in the liver, kidney and skeletal muscle.
  • 數(shù)據(jù)庫鏈接:

    KEGG: mmu:21832

    STRING: 10090.ENSMUSP00000075756

    UniGene: Mm.3943



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