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  4. Mouse Stanniocalcin-1(STC1) ELISA kit

Mouse Stanniocalcin-1(STC1) ELISA kit

  • 中文名稱：
    小鼠斯鈣素-1(STC1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL022821MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠斯鈣素-1(STC1)酶聯免疫試劑盒（CSB-EL022821MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的STC1含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，支持多種科研場景應用，包括探究STC1在代謝調控、細胞應激反應中的表達規律，分析疾病模型中STC1的分泌動態，以及評估藥物或基因干預對靶蛋白水平的影響本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Stc1 ELISA Kit; Stc ELISA Kit; Stanniocalcin-1 ELISA Kit; STC-1 ELISA Kit
  • 縮寫：
    STC1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse STC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 87-97
    1:2 Average % 97
    Range % 95-102
    1:4 Average % 101
    Range % 95-105
    1:8 Average % 94
    Range % 91-98
  • 回收率：
    The recovery of mouse STC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 87-98
    EDTA plasma (n=4) 99 95-104
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.558 2.633 2.596 2.387
    2000 2.205 2.212 2.209 2.000
    1000 1.682 1.665 1.674 1.465
    500 0.990 0.995 0.993 0.784
    250 0.499 0.512 0.506 0.297
    125 0.390 0.421 0.406 0.197
    62.5 0.299 0.322 0.311 0.102
    0 0.205 0.212 0.209 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
  • 基因功能參考文獻：
    1. Latanoprost-induced reduction of intraocular pressure is mediated through the downstream signaling molecule STC-1. When used by itself, STC-1 exhibits ocular hypotensive properties. PMID: 28538979
    2. STC-1 maybe improve anti-inflammation, anti-oxidant and anti-apoptosis activities by affecting reactive oxygen species -mediated pathways, especially the phospho-modifications of the respective proteins, resulting in the increase of Superoxide dismutase and reduce of capase-3, p53, IL-6 and IFN-gamma. PMID: 27420048
    3. STC1 blunts bleomycin-induced rise in thrombin protein and activity, diminishes thrombin-induced signaling through PAR1 to ERK, and inhibits bleomycin-induced pneumonitis. PMID: 26640170
    4. Stanniocalcin 1 is expressed in thyroid side population cells and thyroid cancer cells. PMID: 25647164
    5. Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using Stc1. PMID: 25373521
    6. Stanniocalcin-1 inhibits renal ischemia/reperfusion injury via an AMP-activated protein kinase-dependent pathway. PMID: 25012175
    7. STC1 is an endogenous stress protein that may counteract LPS-induced lung injury by inhibiting the inflammatory cascade and inducing antioxidant and antiapoptotic mechanisms. PMID: 24685991
    8. critical for kidney tubular epithelial survival under physiologic conditions PMID: 24700878
    9. Remarkably, X-linked genes are not overexpressed in female Stc1(-/-) mice, revealing the existence of a mechanism(s) that can compensate for a persistent XCI deficiency to regulate X-linked gene expression. PMID: 25136103
    10. Overexpression of stanniocalcin-1 inhibits reactive oxygen species and renal ischemia/reperfusion injury. PMID: 22695329
    11. STC1 was not crucial for the development of ischemic tolerance triggered by hypoxic preconditioning, or for preserving blood-brain barrier integrity but may be involved in functional recovery after stroke. PMID: 23159313
    12. the inner medullary STC-1 gene is differentially regulated in rat and mouse by arginine vasopressin and angiotensin II PMID: 21867741
    13. Vascular endothelial growth factor-D stimulates endothelial cell VEGF-A, stanniocalcin-1, and neuropilin-2 and has potent angiogenic effects. PMID: 21474827
    14. STC-1 could have a role in salt and water balance as dehydration necessitates water conservation as well as controlled natriuresis and kaliuresis. PMID: 20540985
    15. the biological repertoires of STCs in mammals will be considerably larger than in fish and may not be limited to mineral metabolism PMID: 11832417
    16. STC1 is expressed in a time- and cell-specific manner and may play an autocrine/paracrine role during osteoblast development and bone formation PMID: 11897801
    17. STC-1 can affect calcium homeostasis, bone and muscle mass and structure, and angiogenesis PMID: 12193584
    18. Data characterize the STC receptor and the functional targeting of ligand and receptor to mitochondria. PMID: 12223480
    19. STC1 may play a selective modulatory role in angiogenesis, possibly serving as a "stop signal" or stabilizing factor contributing to the maturation of newly formed blood vessels PMID: 14500721
    20. role for mammalian STC1 in modulating the immune/inflammatory response in ureteric obstruction PMID: 14570698
    21. kinetics of upregulated STC-1 expression during adipogenesis was investigated in 3T3-LI cells PMID: 15149855
    22. These findings have thus shown that the mammary glands are indeed targeted by STC, even in the virgin state. PMID: 16150955
    23. Stc1 function is not essential for growth or reproduction in the mouse PMID: 16287871
    24. systemically derived STC-1 has important effects on mammary gland development and the transfer of serum-based triglycerides into milk and also has a role in maternal behavior PMID: 16772321
    25. Hypoxic preconditioning leads to neuroprotection through IL-6-mediated upregulation of stanniocalcin-1 expression. PMID: 17272771
    26. IL-6-induced expression of STC-1 is one molecular mechanism behind the ischemic tolerance generated by HOPC in the heart PMID: 17573464
    27. stanniocalcin-1 is a potent anti-inflammatory and renal protective protein. PMID: 19246645
    28. Stc1 acts in a negative feedback loop in the prosurvival ERK1/2 signaling pathway during oxidative stress. PMID: 19347030
    29. Stanniocalcin-1 suppresses superoxide generation in macrophages through induction of mitochondrial UCP2. PMID: 19602668
    30. Transgenic overexpression of hSTC1 results in severe post-natal dwarfism (30-50%) indepedent of GH or IGF-I and a reduction female reproductive competence. PMID: 11861508

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Stanniocalcin family
  • 組織特異性：
    Expressed in many tissues.
  • 數據庫鏈接：


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