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Mouse Semaphorin-3A(SEMA3A) ELISA kit

  • 中文名稱:
    小鼠腦信號蛋白3A(SEMA3A)酶聯免疫試劑盒
  • 貨號:
    CSB-EL020980MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠腦信號蛋白3A(SEMA3A)酶聯免疫試劑盒(CSB-EL020980MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的SEMA3A含量。SEMA3A屬于分泌型蛋白,在神經系統發育中發揮導向作用。研究發現其與腫瘤、炎癥等疾病相關。機制上,SEMA3A可與受體結合,調控細胞信號通路,影響細胞遷移、增殖和血管生成等過程,是潛在的藥物作用靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,該產品為神經科學、腫瘤生物學等領域的基礎研究提供工具,支持細胞模型、動物模型中SEMA3A功能探索及信號通路研究,適用于體外實驗的蛋白表達檢測與調控機制分析。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Sema3a ELISA Kit; Semad ELISA Kit; SemD ELISA Kit; Semaphorin-3A ELISA Kit; Semaphorin III ELISA Kit; Sema III ELISA Kit; Semaphorin-D ELISA Kit; Sema D ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 88  
    Range % 84-92  
    1:200 Average % 94  
    Range % 90-98  
    1:400 Average % 99  
    Range % 95-104  
    1:800 Average % 102  
    Range % 96-108  
  • 回收率:
    The recovery of mouse SEMA3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 93-96  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.510 2.568 2.539 2.392  
    500 2.361 2.212 2.287 2.140  
    250 1.869 1.848 1.859 1.712  
    125 1.247 1.226 1.237 1.090  
    62.5 0.648 0.669 0.659 0.512  
    31.2 0.371 0.387 0.379 0.232  
    15.6 0.257 0.261 0.259 0.112  
    0 0.146 0.148 0.147    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty.
  • 基因功能參考文獻:
    1. These findings indicate a regulatory mechanism and a proapoptotic function of aberrant Sema3A signaling in Osteoarthritis (OA) chondrocytes, and suggest that targeting Sema3A signaling might interfere OA pathogenesis. PMID: 29111592
    2. study demonstrates that Sema3E/plexinD1 inhibits proliferation and migration of vascular smooth muscle cells via inactivation of Rap1-AKT signalling pathways PMID: 29016743
    3. SEMA3A expression is increased in the endothelin receptor-B null mouse model of Hirschsprung disease, suggesting that SEMA3A may interfere with enteric neural crest cell migration, resulting in an absence of enteric neurons. PMID: 29224790
    4. Our results demonstrated that overexpression of Sema3A in oral cancer cells drastically suppressed tumor growth by inhibiting angiogenesis. PMID: 28683823
    5. a novel role for Sema3A-neuropilin 1 signalling in progenitor cell dynamics and in the generation of interneurons in the ventral telencephalon PMID: 27858201
    6. Sema3A reduces cardiac inflammation and improves cardiac function after myocardial infarction by promoting the resolution of inflammation. PMID: 28540528
    7. The findings highlight an active role for satellite cell-secreted Sema3A ligand as a key "commitment factor" for the slow-fiber population during muscle regeneration. PMID: 28480592
    8. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure PMID: 27787851
    9. Sema3a-Nrp2 signaling along an unrecognized pancreatic developmental axis constitutes a chemoattractant system essential for generating the hallmark morphogenetic properties of pancreatic islets. PMID: 28893946
    10. These data suggest that both Nrp1 and Sema3a are expressed in the cochlea during time points when spiral ganglion cell begin to innervate the organ of Corti. PMID: 29059194
    11. We show that PTP-3, a LAR homolog in Caenorhabditis elegans, participates in Sema2A-regulated axon guidance. PTPdelta, a member of vertebrate LAR class PTPs, is involved in Sema3A-regulated cortical dendritic growth. In Sema3A signaling, PTPdelta activates Fyn and Src kinases by dephosphorylating their C-terminal Tyr residues. PMID: 28637841
    12. Investigated the effects of BotoxA-induced paralysis on the sprouting capacity of neuromuscular junctions (NMJs) in the K108N-SEMA3A mutant, and observed no change in the differential neuronal plasticity found at NMJs on fast-fatigable or slow muscle fibers due to the presence of the SEMA3A mutant protein. PMID: 28103314
    13. The aim was to elucidate whether ectopic muscles are a result of misguidance of myoblast precursors due to the loss of Sema3A-Npn-1 signaling. PMID: 27466379
    14. These findings suggest that by interacting with PlexA4, TrkA plays a crucial role in redirecting local Sema3A signaling to retrograde axonal transport, thereby regulating dendritic GluA2 localization and patterning. PMID: 26945060
    15. Findings indicate that Rho and ROCK knockout may improve the behavior of mice and prevent MPTP-induced dopaminergic neurons damage by regulating Sema3A, PlexinA and NRP-1 in a mouse model of Parkinson's disease. PMID: 27772760
    16. Sema3A signaling exerts its oncogenic effect by promoting an mTORC1-mediated metabolic shift from oxidative phosphorylation to aerobic glycolysis. PMID: 27246732
    17. Semaphorin 3A (Sema3A) is a protein secreted during the development of the nervous system that acts as a cue for axon induction . PMID: 28013333
    18. this paper shows that Nav1.7, by coupling with CRMP1, mediates the axonal retrograde signaling of Sema3A in axonal guidance PMID: 28254884
    19. miR-30c and sema3A regulate adult neurogenesis by controlling proliferation and differentiation of stem cells in the subventricular zone. PMID: 27198082
    20. These findings indicate that endothelial cell-derived Sema3A exerts repelling functions on VEGF-A-induced tip cell filopodia and that a lack of this signaling cannot be rescued by paracrine sources of Sema3A. PMID: 26884395
    21. Data suggest that sema3A is involved in the pathogenesis of experimental autoimmune encephalomyelitis, in the modulation of the immune response and in the neurodegeneration that take place in the CNS. PMID: 26686462
    22. these studies demonstrated that Sema3A is a key mediator of cerebrovascular permeability and contributes to brain damage caused by cerebral ischemia. PMID: 25601765
    23. Diabetes induced sema3A excretion in urine. Increased semaphorin 3A was associated with severity of albuminuria. Seme3A-mediated diabetes induced glomerulosclerosis. PMID: 25249008
    24. These findings indicate that excess sema3a promotes severe diabetic nephropathy and identifies novel potential therapeutic targets for diabetic nephropathy. PMID: 25475434
    25. interleukin-4 and tumor necrosis factor-alpha stimulation increases epidermal innervation and suppresses semaphorin 3A PMID: 25088775
    26. The Sema3A/Npn1 pathway participates to the parcellation of the visual neocortex in both the mouse. PMID: 23709645
    27. Data suggest that loss of semaphorin-3A (Sema3A)-neuropilin 1 (Npn1) signaling is not the only cause for the developmental deficits. PMID: 25512301
    28. Sema3A is an essential, locally produced chemorepellant, which by creating mesenchymal exclusion areas, regulates the timing and patterning of the dental nerves during the development of incisor tooth germ. PMID: 24752460
    29. Sema3A exacerbates acute kidney injury via promoting inflammation and epithelial cell apoptosis. PMID: 24829504
    30. Blockade of Sema3A signaling via lens removal or injection of a synthetic Sema3A inhibitor causes ectopic migration of angioblasts into the cornea and results in its subsequent vascularization. PMID: 24809797
    31. This study examines the hypothesis that Sema3A signaling through its motor neuron neuropilin-1 (NRP1) receptor triggers distal axonopathy PMID: 24918638
    32. genetic findings demonstrate that Sema3a repellent signaling plays a role in the establishment of proper afferent projections in SAG neurons, and this signaling likely occurs through a receptor complex involving Npn1 and either plexinA1 or plexinA3 PMID: 23991118
    33. these results suggest that Sema3A and Sema7A have opposite effects and are involved in different stages of synapse elimination. PMID: 24831527
    34. A novel direct interaction between the Sema3a signaling receptor plexinA1 and nephrin, linking extracellular Sema3a signals to the slit-diaphragm signaling complex, was identified. PMID: 23954273
    35. Sema3A is robustly induced in the early stages of diabetes and mediates, via Neuropilin-1, the breakdown of the inner blood-retinal barrier. PMID: 24093675
    36. Semaphorin 3A is expressed in lymphatic vessels and interacts with neuropilin 1 in lymphangiogenesis [review] PMID: 24276892
    37. Increased Levels of Axon Guidance Errors are Detected in Sema3A+/+:BAX-/- and Sema3A+/-:BAX-/- Mice. PMID: 23922915
    38. Sema3A may play a key role in the proper development of the dorsal ramus projection. PMID: 23998265
    39. shows that TAMs' heterogeneity depends on their localization, which is tightly controlled by Sema3A/Nrp1 signaling PMID: 24332039
    40. results demonstrate a critical role of local SEMA3A signaling in layer-specific axonal branching, which contributes to target innervation PMID: 23602477
    41. Studied the therapeutic effect of endostar with cisplatin and investigated the inhibiting mechanism of endostar from tumor angiogenesis. Expressions of VEGF and Sema3A were evaluated by immunhistochemisty. PMID: 23317887
    42. study demonstrates that Sema3A regulates bone remodelling indirectly by modulating sensory nerve development, but not directly by acting on osteoblasts PMID: 23644455
    43. Sema3A overrides the effects of Sema3C in cortical axons exposed to defined mixtures of these opposing guidance cues in vitro PMID: 22368082
    44. Semaphorin 3A impinged upon the basal activity of the serine phosphatase PP2A and disrupted PP2A interaction with VE-cadherin, leading to cell-cell junction disorganization and increased permeability. PMID: 22685328
    45. These findings indicate a critical role for Sema3-Nrp1 signaling in fetal pulmonary development. PMID: 23063659
    46. There was an approximately 40% increase in the number of branching points and in the length of the dendrites in Sema3A-treated CRMP4+/+ hippocampal neurons. PMID: 22234963
    47. Following middle cerebral artery occlusion to induce stroke, immunohistochemistry shows both Sema3A and 12/15-LOX are increased in the cortex up to 2 wk. PMID: 23070608
    48. Expression patterns of Sema3A indicate that it may control the timing and patterning of the incisor innervation. Sema3A appears to regulate innervation of the periodontal ligament. PMID: 21793640
    49. Data demonstrate an essential direct function of Sema3A-Nrp1-PlexinA1 signaling in lymphatic valve formation. PMID: 22723296
    50. Sema3A-Nrp-1 signaling directs the maturation of the lymphatic vascular network likely via regulating the perivascular cell coverage of the vessels thus affecting lymphatic vessel function and lymphatic valve development. PMID: 22723300

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Semaphorin family
  • 數據庫鏈接:


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