Mouse Renin ELISA Kit
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中文名稱:小鼠腎素(Renin)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08703m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠腎素(Renin)酶聯(lián)免疫試劑盒(CSB-E08703m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的REN含量。小鼠腎素(Renin)是一種由腎臟分泌的酶,它在生理上通過(guò)裂解肝臟產(chǎn)生的血管緊張素原,激活腎素-血管緊張素-醛固酮系統(tǒng)(RAAS),從而調(diào)節(jié)血壓和體液平衡。研究顯示,腎素在高血壓腎損害中扮演重要角色,其表達(dá)與腎纖維化和炎癥反應(yīng)密切相關(guān),可能成為治療高血壓腎損害的新靶點(diǎn)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL,適用于小鼠模型的高通量篩選、腎臟病理機(jī)制研究、心血管疾病相關(guān)實(shí)驗(yàn)以及藥物干預(yù)后Renin水平變化的動(dòng)態(tài)分析本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名:Ren1 ELISA Kit; Ren ELISA Kit; Ren-1 ELISA Kit; Renin-1 ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit; Kidney renin ELISA Kit
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縮寫:Renin
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 81-90 1:2 Average % 94 Range % 90-100 1:4 Average % 99 Range % 92-108 1:8 Average % 92 Range % 84-98 -
回收率:
The recovery of mouse renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 83-98 EDTA plasma (n=4) 89 82-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 3.020 2.952 2.986 2.853 500 2.486 2.296 2.391 2.258 250 1.712 1.604 1.658 1.525 125 1.031 0.958 0.995 0.862 62.5 0.637 0.624 0.631 0.498 31.2 0.414 0.382 0.398 0.265 15.6 0.264 0.248 0.256 0.123 0 0.137 0.129 0.133 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
相關(guān)問(wèn)答
■ 常見(jiàn)問(wèn)題解答
Q:
Please quote Mouse plasma renin activity assays.
A:
Thanks for your inquiry. We have CSB-E08703m available.It is for quantitative determinationcan but it can not be used to test the activity. I will send you the kit manual by email and ask our distributor in your area to give you a quote soon.
靶點(diǎn)詳情
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功能:Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
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基因功能參考文獻(xiàn):
- This study reveals that the human renin protein is able to complement the mouse Ren1d(-/-) non-granulated defect and suggests that granulopoiesis requires a structural motif that is conserved between the mouse Ren1d and human renin proteins. PMID: 29123029
- These observations demonstrate that activation of the renin-angiotensin system (RAS) promotes colitis in a blood pressure independent manner. Angiotensin II appears to drive colonic mucosal inflammation by promoting intestinal epithelial cell apoptosis and mucosal TH17 responses in colitis development. PMID: 27271344
- Mice homozygous for the Ren1c gene disruption (Ren1c-/-) display several morphological and physiological abnormalities, including poorly developed renal medulla, papillary atrophy, hydronephrosis, inability to concentrate the urine, polyuria, polydipsia, renal failure, and anemia. PMID: 28533331
- renin inhibition with aliskiren leads to improved ischemia-induced neovascularization that is not dependant on blood pressure lowering PMID: 26295797
- Calcineurin inhibitors induce production of renin in the collecting ducts that may contribute to decreased renal blood flow and increased VEGF production. PMID: 26502369
- AKR1B7 is expressed within the renin cell throughout development and perturbations to homeostasis, and AKR1B7 is regulated by cAMP levels within the renin cell. PMID: 26180185
- Characterize hypertension produced by transduction with a renin1-coding adeno-associated virus. PMID: 26108870
- Cx40 is a requirement for the pressure control of renin secretion irrespective of the localization of renin secreting cells. PMID: 25241776
- Deletion of renin in the vasculature resulted in neonatal mortality that could be rescued with daily injections of saline PMID: 26246508
- Cells of renin lineage (CoRL) serve as progenitors for other cells in kidney disease characterized by both glomerular and tubulointerstitial injury. PMID: 26062877
- Specific cross-linking of the high affinity immunoglobulin epsilon receptor on the surface of bone marrow-derived mast cells and heart mast cells elicits the release of enzymatically active renin protein. PMID: 24262755
- Blood pressure homeostasis is regulated through transcriptional cis elements controlling renin activity. PMID: 24734888
- extraglomerular renin lineage cells represent a major source of repopulating cells for reconstitution of the intraglomerular mesangium after injury PMID: 24904091
- Data indicate the role of renin in hypertension linked to loss of connexin 40 (Cx40). PMID: 24614215
- Renin secretory granules possess integral lysosomal proteins, confirming that they are indeed modified lysosomes PMID: 23635510
- Data indicate that in 1-day-old Task3-/- mice, renal renin concentration was lower than in wild-type mice. PMID: 23698720
- Overexpression of renin in the kidney collecting duct elevated blood pressure. Results suggest that collecting duct-derived renin has the potential to modulate blood pressure independent of the systemic renin-angiotensin system PMID: 23702969
- renin is released by mechanisms similar to compound exocytosis. PMID: 23235568
- Olfactory receptor responding to gut microbiota-derived signals plays a role in renin secretion and blood pressure regulation. PMID: 23401498
- deletion of Vhl shifts the phenotype of juxtaglomerular cells from a renin- to erythropoietin-secreting cell type, presumably in response to HIF-2 accumulation PMID: 23393316
- The enhanced expression of bone renin and angiotensin II plays an important role in the pathology of age-related osteoporosis. PMID: 22785482
- Data show that COUP-TFII is involved in the control of renin gene expression. PMID: 22645148
- the mouse-renin gene is regulated by apurinic/apyrimidinic-endonuclease 1 (APE1/Ref-1) via recruitment of histone deacetylase 1 corepressor complex PMID: 22441348
- Changes in preglomerular blood pressure may be an important mediator of the influence of salt intake on the number and distribution of renin-producing cells in the kidney. PMID: 22357914
- Data suggest that Western diets (i.e., diets high in saturated fat and or salt) down-regulate expression of renin in kidney. PMID: 21499941
- It was concluded that both Nr2f2 and Nr2f6 negatively regulate renin promoter activity, but may do so by divergent mechanisms. PMID: 22278040
- VAMP2 and VAMP3 are expressed in JG cells, but only VAMP2 is targeted to renin-containing granules and mediates the stimulatory effect of cAMP on renin exocytosis. PMID: 21708949
- genetic disruption of beta-adrenergic receptors reduces basal renin expression along the developing preglomerular tree and in adult kidneys PMID: 21389089
- Renin-angiotensin-aldosterone system components are upregulated under endothelin blockade. PMID: 20093323
- cathepsin B-deficient mice exhibit no differences compared with controls in their ability to generate active renin, and our results do not support CTSB as the processing enzyme in mice PMID: 20164204
- Altered renin synthesis and secretion in the kidneys of heterozygous mice with a null mutation in the TGF-beta(2) gene. Renal renin mRNA and protein levels were higher in mutant mice under hydrated or dehydrated conditions. PMID: 12381922
- Review. Role of the local renin-angiotensin system in cardiac damage, focussing on transgenic animal models. PMID: 12431444
- identification of several binding sites within the renin enhancer region that are essential for high level expression of Ren-1 gene PMID: 12591615
- renin transcription is regulated by TNFalpha, which is regulatedby NFkappaB PMID: 14563845
- aldosterone exerts a direct positive effect on renin gene expression at the cellular level probably by stabilizing renin mRNA. PMID: 14583438
- NO independent of its exact source permits the macula densa pathway of renin secretion to function normally. PMID: 15075180
- Transgenic rats overexpressing the mouse Ren1 gene developed myocardial hypertrophy independent of calcineurin. PMID: 15322704
- Review. Transcription of renin genes is subject to complex developmental and tissue-specific regulation. A sequence highly homologous to the mouse enhancer is also found in the human and rat renin genes. PMID: 15545507
- renin is the only enzyme capable of maintaining plasma angiotensins and that renin expression in the kidney is very tightly regulated at the mRNA level. PMID: 15563565
- LXR(alpha) and LXR(beta) regulate renin expression in vivo by directly interacting with the renin promoter PMID: 16007255
- the renin enhancer is critical for renin gene expression and physiological sequelae, including response to alteration in salt intake PMID: 16895910
- Stimulation of renin by furosemide was not affected by mPGES1 deficiency. PMID: 17442791
- The renin enhancer located 2.7 kb upstream of the renin gene is critical for replenishment of renin stores and response in renin to a chronic in vivo stimulus PMID: 17846348
- This study describes the three-dimensional development of renin expression in mouse kidneys during fetal and postnatal life. PMID: 17898695
- Ren1c(-/-) mice are lean, insulin sensitive, and resistant to diet-induced obesity without changes in food intake and physical activity. The lean phenotype is likely due to a higher metabolic rate and gastrointestinal loss of dietary fat. PMID: 18054319
- This study showed that the Ren-1c enhancer does not regulate the rate of promoter activity, but rather increases the probability of achieving an active transcriptional state. PMID: 18307722
- Connexin45 is expressed in the juxtaglomerular apparatus and is involved in the regulation of renin secretion and blood pressure. PMID: 18579650
- expression of the human VDR in mouse juxtaglomerular cells reduced renin expression without affecting calcium or parathyroid hormone status PMID: 19034301
- Continuous activation of the brain renin-angiotensin system impairs cognitive function via stimulation of the angiotensin II type 1 receptor with a decrease in cerebral surface blood flow and an increase in oxidative stress. PMID: 19047580
- CBP and p300 are essential for renin cell identity and morphological integrity of the kidney. PMID: 19252086
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亞細(xì)胞定位:Secreted. Membrane.
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蛋白家族:Peptidase A1 family
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組織特異性:Kidney.
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