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  4. Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit

  • 中文名稱：
    小鼠再生胰島衍生蛋白3γ(REG3G）酶聯免疫試劑盒
  • 貨號：
    CSB-EL019549MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠再生胰島衍生蛋白3γ(REG3G）酶聯免疫試劑盒（CSB-EL019549MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的REG3G含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，科研人員可將其應用于小鼠模型研究，如胰腺再生機制探索、腸道炎癥性疾病進程分析或感染后宿主防御反應評估，支持體外實驗樣本中REG3G的動態濃度檢測。為分子機制研究和生物標志物發現提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Reg3g ELISA Kit; Pap3 ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; REG-3-gamma ELISA Kit; Pancreatitis-associated protein 3 ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; Reg III-gamma) [Cleaved into: Regenerating islet-derived protein 3-gamma 16.5 kDa form; Regenerating islet-derived protein 3-gamma 15 kDa form] ELISA Kit
  • 縮寫：
    REG3G
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %93
    Range %89-106
    1:200Average %92
    Range %83-100
    1:400Average %95
    Range %89-99
    1:800Average %97
    Range %89-103
  • 回收率：
    The recovery of mouse REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-98
    EDTA plasma (n=4)9792-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.190 2.123 2.157 1.996
    5001.793 1.762 1.778 1.617
    2501.295 1.257 1.276 1.115
    1250.779 0.748 0.764 0.603
    62.50.562 0.554 0.558 0.397
    31.250.375 0.368 0.372 0.211
    15.60.263 0.254 0.259 0.098
    00.157 0.165 0.161
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：Serum ,細胞培養上清液

樣品信息：小鼠

稀釋比：其他

產品評價： 我使用Mouse Reg3genRating islet-derived protein 3-gamma（REG3G)ELISA kit，檢測小鼠血清內REG3G,分別檢測了1：50，1：100，1：200的稀釋比例。以1：200為例，對照組和實驗組的OD值分別是0.2603和0.4635，實驗結果符合我們對實驗前期的預測。產品評價：該elisa檢測試劑盒產品質量過關，檢測效果好。能較好的滿足實驗需求。

By 吳老師

靶點詳情

  • 功能：
    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
  • 基因功能參考文獻：
    1. the secretion of regenerating islet-derived IIIgamma (RegIIIgamma) is responsible for the protective effect of Adipose-derived stem cells. PMID: 26866937
    2. The study shows that cardiac stress activates Reg3gamma expression and p38 MAPK is an upstream regulator of Reg3gamma gene expression in heart. Altogether the data suggest Reg3gamma is associated with cardiac inflammatory signaling. PMID: 27798352
    3. regenerating islet-derived gene gamma promotes beta cell regeneration and preserves beta cells from autoimmunity damage by increasing regulatory T cell differentiation and inducing tolerated dendritic cells. PMID: 26667474
    4. Recombinant IFN-I or IFN-I expression induced by RIG-I promoted growth of intestinal organoids in vitro and production of the antimicrobial peptide regenerating islet-derived protein 3 gamma (RegIIIgamma). PMID: 28424327
    5. REG3gamma-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum. PMID: 24345802
    6. antibacterial function for lung epithelium through Stat3-mediated induction of Reg3gamma. PMID: 23401489
    7. Reg-IIIgamma expression is mainly observed in epithelial cells of the airways and intestine, but not in the nervous system; expression levels show a gradually increasing pattern along with development. PMID: 21681751
    8. Letter: aggregation status of PAP1 and PAP2 could change their role in regulating the inflammation in acute pancreatitis. PMID: 21926556
    9. findings show that RegIIIgamma is essential for maintaining a ~50-micrometer zone that physically separates the microbiota from the small intestinal epithelial surface PMID: 21998396
    10. findings show that RegIIIgamma and its human counterpart, HIP/PAP, are directly antimicrobial proteins that bind their bacterial targets via interactions with peptidoglycan carbohydrate PMID: 16931762
    11. antibacterial activities of mouse RegIIIgamma and its human ortholog, HIP/PAP, are tightly controlled by an inhibitory N-terminal prosegment that is removed by trypsin in vivo. PMID: 19095652

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  • 亞細胞定位：
    Secreted. Cytoplasm.
  • 組織特異性：
    Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection (at protein level). Skin
  • 數據庫鏈接：


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