在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Mouse R-spondin-2(RSPO2) ELISA kit

  • 中文名稱:
    小鼠R脊椎蛋白2(RSPO2)酶聯免疫試劑盒
  • 貨號:
    CSB-EL020551MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠R脊椎蛋白2(RSPO2)酶聯免疫試劑盒(CSB-EL020551MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的RSPO2含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于體外定量分析小鼠模型或細胞實驗中RSPO2的表達水平變化,為研究其在發育生物學、疾病機制或信號通路調控中的功能提供可靠工具。該產品嚴格用于科學研究,不適用于臨床診斷或其他用途。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Rspo2R-spondin-2 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 2 ELISA kit; Cristin-2 ELISA kit; mCristin-2 ELISA kit; Roof plate-specific spondin-2 ELISA kit
  • 縮寫:
    RSPO2
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Developmental Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 97    
    Range % 92-102    
    1:2 Average % 91    
    Range % 85-98    
    1:4 Average % 90    
    Range % 88-93    
    1:8 Average % 100    
    Range % 96-104    
  • 回收率:
    The recovery of mouse RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-92    
    EDTA plasma (n=4) 106 103-110    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.522 2.633 2.578 2.472    
    1000 2.123 2.213 2.168 2.062    
    500 1.579 1.544 1.562 1.456    
    250 1.020 0.989 1.005 0.899    
    125 0.744 0.688 0.716 0.610    
    62.5 0.460 0.432 0.446 0.340    
    31.25 0.203 0.214 0.209 0.103    
    0 0.102 0.109 0.106      
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form.
  • 基因功能參考文獻:
    1. Data report that Rspo2, is highly expressed in the spinal motor neuron (SMN) and binds to its receptor Lgr5, at the neuromuscular junction (NMJ). Rspo2 enhances the LRP4/MuSK signaling via Lgr5 in an agrin-independent manner and promotes AChR clustering. In addition, the loss of Rspo2 in mice compromises AChR clustering, the ultrastructure of the NMJ, and neuromuscular signal transduction. PMID: 27328992
    2. Rspo2 activates Wnt/beta-catenin signaling to reduce Col2a1 and Sox9 and to facilitate differentiation of proliferating chondrocytes into hypertrophic chondrocytes in growth cartilage PMID: 27012200
    3. High resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2. PMID: 26123262
    4. These findings suggest that R-Spondin2 may promote hepatic stellate cell activation by enhancing the canonical Wnt pathway. PMID: 24852883
    5. R-spondin2 is a key molecular link between infection and intestinal homoeostasis. PMID: 23695692
    6. R-spondin 2 homolog (Xenopus laevis) is identified as an essential regulator of Citrobacter rodentium infection susceptibility in mice PMID: 24005619
    7. Oocyte-derived R-spondin2 is a paracrine factor essential for primary ovarian follicle development PMID: 23407710
    8. Rspo2 is required to limit the number of outer hair cells to three rows and for optimal arrangement of peripheral nerve fibers in the developing cochlea. PMID: 23192966
    9. Rspo2 mutation in combination with Rspo3 mutation causes severe limb truncation. PMID: 22610508
    10. Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells. PMID: 22238613
    11. Results indicate that Rspo2 and Wnt1 have mutually distinct effects on mammary epithelial cell growth and these effects are context-dependent. PMID: 21732367
    12. Rspo2 is a mesenchyme-derived factor that plays critical roles in regulating branchial arch 1 patterning and morphogenesis through ectodermal-mesenchymal interaction and a novel genetic factor for cleft palate. PMID: 21237142
    13. The findings suggest that Rspo2 expression in the apical ectodermal ridge (AER) is required for AER maintenance likely by regulating Wnt/beta-catenin signaling. PMID: 17904116
    14. This study shows that Rspo2 is critical for maintenance of the AER and for growth and patterning in limb development. PMID: 18067586
    15. Lrp6-mediated R-spondin 2 signaling through the canonical Wnt pathway is required for normal morphogenesis of the respiratory tract and limbs. PMID: 18256198
    16. Wnt11 signals through beta-catenin, activating Rspo2 expression, which is then required for Wnt11-mediated osteoblast maturation. PMID: 19213727
    17. These findings indicate that Rspo2 regulates midfacial, limb, and lung morphogenesis during development through the Wnt/beta-catenin signaling. PMID: 19233133
    18. The Rspo2 mutants had markedly reduced potency relative to the wild-type protein,demonstrating for the first time specific amino acid residues in Rspos that are critical for beta-catenin signaling. PMID: 19385064

    顯示更多

    收起更多

  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    R-spondin family
  • 數據庫鏈接:


主站蜘蛛池模板: 亚洲欧美中文日韩在线视频一 | 第一亚洲中文久久精品无码| 亚洲日韩欧美在线成人| 人人妻人人澡人人爽人人精品电影| 无码专区一va亚洲v专区在线| 日本熟妇人妻中出| 忘忧草社区中文字幕www| 亚洲乱码在线卡一卡二卡新区豆瓣| 亚洲国产成人精品激情姿源| 国产成人a人亚洲精v品无码| 欧美老妇大p毛茸茸| 亚洲精品55夜色66夜色| 久久久久久无码精品人妻a片软件 999久久久精品国产消防器材 | 国产精品无码翘臀在线看| 两个人看的www免费视频中文| 人妻中文无码久热丝袜| 国产精品亚洲w码日韩中文| 换脸国产av一区二区三区| 老司机午夜精品视频资源| 成 人 网 站 免费观看| 欧美影院成年免费版| 欧美人与动欧交视频| 久久综合九色综合97婷婷| 性xxx欧美老妇5060.70| 日韩一区二区三区无码人妻视频| 性xxxx视频播放免费| 国产免费一区二区三区免费视频 | 亚洲精品无码成人av电影网| 中文字幕美人妻亅u乚一596| 亚洲国产成人久久一区www| 中文乱码字幕视频观看网站免费| 国产美女亚洲精品久久久| 国产成人久久av免费| 亚洲人成电影在线观看影院| 午夜私人成年影院| 国产又黄又湿无遮挡免费视频| 久久无码精品一区二区三区| 肥臀浪妇太爽了快点再快点| 亚洲在av人极品无码| 精品伊人久久久大香线蕉下载| 亚洲一区二区三区|