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  4. Mouse Protein notum homolog(NOTUM) ELISA kit

Mouse Protein notum homolog(NOTUM) ELISA kit

  • 中文名稱：
    小鼠notum蛋白同源物(NOTUM)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL015955MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠notum蛋白同源物(NOTUM)酶聯(lián)免疫試劑盒（CSB-EL015955MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的NOTUM含量。NOTUM是一個(gè)重要靶點(diǎn)。它是分泌性蛋白，可調(diào)節(jié)Wnt信號(hào)通路。異常激活的Wnt信號(hào)與多種疾病相關(guān)，如腫瘤、纖維化等。對(duì)NOTUM的研究，主要圍繞其對(duì)Wnt信號(hào)的調(diào)控機(jī)制，期望通過(guò)干預(yù)該靶點(diǎn)為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于體外研究NOTUM在胚胎發(fā)育、干細(xì)胞分化以及骨代謝相關(guān)疾病模型中的表達(dá)動(dòng)態(tài)，尤其適合評(píng)估不同處理?xiàng)l件下（如基因編輯、藥物干預(yù)等）細(xì)胞或組織內(nèi)源性NOTUM的分泌水平變化，為探索Wnt信號(hào)通路調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    NotumPalmitoleoyl-protein carboxylesterase NOTUM ELISA Kit; EC 3.1.1.98 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %109
    Range %104-113
    1:2Average %93
    Range %89-99
    1:4Average %100
    Range %96-104
    1:8Average %88
    Range %84-92
  • 回收率：
    The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9894-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.779 2.798 2.789 2.587
    52.201 2.156 2.179 1.977
    2.51.484 1.451 1.468 1.266
    1.250.845 0.899 0.872 0.670
    0.6250.555 0.561 0.558 0.356
    0.3120.432 0.445 0.439 0.237
    0.1560.312 0.326 0.319 0.117
    00.201 0.203 0.202
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
  • 基因功能參考文獻(xiàn)：
    1. Notum influences mesenchymal cell differentiation by generating a barrier for Wnt ligands produced and secreted by airway epithelial cells to attenuate Wnt signaling. PMID: 29428562
    2. The findings support a significant role for NOTUM in modulating WNT signaling pathways that have pleiotropic effects on tooth and kidney development. PMID: 26926082
    3. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
    4. Male mice deficient in Notum specifically in the liver develop metabolic dysfunctions implicating Notum in the development of Type 2 diabetes. PMID: 26974334
    5. Describes the human ortholog, and analyzes both the human and mouse promoters. PMID: 18429952

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Pectinacetylesterase family, Notum subfamily
  • 組織特異性：
    Widely expressed. Expressed in lung, ovary, kidney, liver and brain. Not detected in thymus, heart, spleen, stomach, skeletal muscle and bone marrow.
  • 數(shù)據(jù)庫(kù)鏈接：


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