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  4. Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

  • 中文名稱：
    小鼠蛋白質(zhì)二硫鍵異構(gòu)酶A4(PDIA4)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL017722MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠蛋白質(zhì)二硫鍵異構(gòu)酶A4(PDIA4)酶聯(lián)免疫試劑盒（CSB-EL017722MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PDIA4含量。PDIA4作為靶點(diǎn)有重要研究意義。其參與蛋白質(zhì)折疊、內(nèi)質(zhì)網(wǎng)應(yīng)激反應(yīng)等過程。研究圍繞其在細(xì)胞內(nèi)的分子作用機(jī)制，探索它與疾病發(fā)生發(fā)展的關(guān)系，如在癌癥等疾病中可能有異常表達(dá)，為藥物研發(fā)、疾病治療靶點(diǎn)的確定提供了方向。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于基礎(chǔ)研究中探索PDIA4在細(xì)胞應(yīng)激反應(yīng)、內(nèi)質(zhì)網(wǎng)功能調(diào)控中的分子機(jī)制，或用于評估疾病模型動(dòng)物體內(nèi)PDIA4表達(dá)水平與病理進(jìn)程的關(guān)聯(lián)性，為蛋白質(zhì)質(zhì)量控制網(wǎng)絡(luò)研究及靶向藥物開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Pdia4 ELISA Kit; Cai ELISA Kit; Erp72Protein disulfide-isomerase A4 ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; ER protein 72 ELISA Kit; ERp-72 ELISA Kit; ERp72 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %81-92
    1:2Average %100
    Range %93-104
    1:4Average %90
    Range %85-95
    1:8Average %99
    Range %91-103
  • 回收率：
    The recovery of mouse PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-96
    EDTA plasma (n=4)9589-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.654 2.543 2.599 2.417
    102.135 2.077 2.106 1.924
    51.345 1.332 1.339 1.157
    2.50.824 0.843 0.834 0.652
    1.250.526 0.545 0.536 0.354
    0.6250.359 0.369 0.364 0.182
    0.3120.278 0.266 0.272 0.090
    00.184 0.180 0.182
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. activated by IL11 in placenta PMID: 28487027
    2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
    3. ERp72 functions separately from protein disulfide isomerase and ERp57 in supporting platelet aggregation. Defective aggregation of ERp72-null platelets was recovered by ERp72, but not other thiol isomerases. PMID: 28576878
    4. A network of endoplasmic reticulum ERp57, ERp72, and ERp29 facilitate polyomavirus infection. PMID: 21159867
    5. There were increased numbers of ERp72-immunoreactive cells in the dorsal and lateral cortex during sleep deprivation. PMID: 12535952
    6. These results suggest that domains 1 and 2 of ERp72 contribute to catalytic efficacy, and domain 3 to binding affinity. PMID: 16333982

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  • 亞細(xì)胞定位：
    Endoplasmic reticulum lumen. Melanosome.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:12304

    STRING: 10090.ENSMUSP00000076521

    UniGene: Mm.2442



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