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Mouse Protein Wnt-3a(WNT3A) ELISA kit

  • 中文名稱:
    小鼠Wnt-3a蛋白(WNT3A)酶聯免疫試劑盒
  • 貨號:
    CSB-EL026136MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠Wnt-3a蛋白(WNT3A)酶聯免疫試劑盒(CSB-EL026136MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的WNT3A含量。WNT3A 是一種重要的信號蛋白。它在胚胎發育、細胞增殖與分化等過程中發揮關鍵作用。其研究機制主要圍繞 WNT 信號通路,異常激活或抑制該通路會引發發育異常及疾病,如腫瘤,對它的深入研究有助于疾病治療手段的探索。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL,適用于科研領域研究Wnt信號通路的激活狀態、干細胞定向分化調控、腫瘤微環境分析或發育生物學模型構建等實驗場景,支持多種生物樣本類型檢測需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Wnt3a ELISA kit; Wnt-3aProtein Wnt-3a ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    23.5 pg/mL-1500 pg/mL
  • 靈敏度:
    5.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Stem Cells
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %90-95
    1:2Average %89
    Range %85-94
    1:4Average %94
    Range %89-97
    1:8Average %98
    Range %95-100
  • 回收率:
    The recovery of mouse WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-95
    EDTA plasma (n=4)9591-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.886 2.864 2.875 2.778
    7502.021 2.011 2.016 1.919
    3751.152 1.132 1.142 1.045
    187.50.645 0.638 0.642 0.545
    940.364 0.378 0.371 0.274
    470.239 0.255 0.247 0.150
    23.50.162 0.172 0.167 0.070
    00.095 0.098 0.097  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
  • 基因功能參考文獻:
    1. This work provides a new link between Wnt3a and macrophage-mediated angiogenesis under glucose and oxygen deprivation in vitro. PMID: 29199847
    2. Lrp6 is the key mediator of Wnt3a signaling in osteoblasts and Lrp5 played a less significant role in mediating Wnt3a signaling. PMID: 29176883
    3. These data define the mechanism responsible for the repressive effects of nitric oxide (NO) on the transcriptional activity of beta-catenin and link eNOS-derived NO to the modulation by VEGF of Wnt/beta-catenin-induced endothelial cell proliferation. PMID: 28320874
    4. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression PMID: 28093227
    5. this study shows that the Wnt3a expression levels in dendritic cells influences the generation of memory T cells after 5 days in co-culture with naive T cells through activation of the Wnt canonical pathway PMID: 27544306
    6. Wnt3a induces Osx expression via p38 MAPK signaling in dental follicle cells. Wnt3a-induced Osx expression was inhibited in the presence of p38 mitogen-activated protein kinase (MAPK) inhibitors (SB203580 and SB202190) at gene and protein levels, as assessed by real-time PCR and immunocytohistochemistry, respectively. PMID: 27450807
    7. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells PMID: 27633082
    8. Wnt3a promotes macrophage-mediated bacterial killing by elevating CRAMP and BD1 levels. PMID: 26846714
    9. Wnt3a acutely reduces nuclear acetyl-CoA, the necessary substrate for histone acetyltransferases, resulting in a global decrease in histone acetylation. PMID: 27129247
    10. Wnt3a rapidly activated the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway and pharmacological stimulation of cAMP/PKA signaling suppressed osteoclast differentiation PMID: 26189772
    11. Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration. PMID: 26414873
    12. Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus. PMID: 26956881
    13. that activation of the Wnt pathway made cells more readily tolerate ROS-caused mitochondria injury and cell apoptosis PMID: 26085341
    14. Pin1 is indispensable for Wnt3a-induced osteoblast differentiation. PMID: 26740630
    15. The mouse xenograft model showed that Wnt3a-overexpressing cells grew into larger tumor masses and formed more vasculogenic mimicry than the control cells. PMID: 26266404
    16. miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression. PMID: 26006230
    17. Wnt8a and Wnt3a cooperate to maintain Fgf8 expression and prevent premature Sox2 up-regulation in the axial stem cell niche, critical for posterior growth. PMID: 25809880
    18. WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo heterologous engraftment. PMID: 25084050
    19. C1q-induced activation of Wnt3a/CTNNB1/FOXO1 signaling contributes to skeletal fiber type shift toward fatigable fiber in congestive heart failure. PMID: 26038536
    20. Study reports that NRF2 participates in the formation of a protein complex with Axin1/GSK-3/beta-TrCP that is disrupted by WNT-3A, a prototypic canonical WNT ligand, and leads to upregulation of the NRF2 transcriptional signature. PMID: 25336178
    21. Wnt3a and MMP9 induce C3H10T1/2 cells differentiation into cardiomyocyte-like cells. PMID: 25854560
    22. Wnt3a/beta-catenin signaling directs trunk progenitors towards paraxial mesodermal progenitor fates; however, our data also suggest that Wnt3a positively supports a progenitor state for both mesodermal and neural progenitors. PMID: 25922526
    23. the presented systems-biology approach demonstrates that Wnt3a attenuates RANKL-driven osteoclastogenesis by blocking c-Fos expression PMID: 25038457
    24. Wnt3a/Frizzled-3 signaling plays important role in regulating the proliferation and differentiation of neural crest cells and various developmental stages of melanocyte precursors. PMID: 24815018
    25. Sp5 and Sp8 are pivotal downstream effectors of Wnt3a/beta-catenin that are essential for the self-renewal and differentiation of neuromesodermal stem cells. PMID: 24475213
    26. results suggest that Wnt3a overrides the effect of P2X7R on the Wnt/beta-catenin signaling to prevent the AEC I death and restrict the severity of ALI. PMID: 24922070
    27. these data identify HES5 as a key mediator of the Wnt-3a proneurogenic effect occurring independently of the classical Wnt/beta-catenin signaling cascade thus further deciphering crosstalk mechanisms of Wnt and Notch signaling pathways regulating cell fate PMID: 24548083
    28. These results indicate that JBP regulates the expressions of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice. PMID: 24841652
    29. our results suggest a dose-dependent role for Wnt3a in regulating the balance between renewal and selection of differentiation fates of axial progenitors in the epiblast. PMID: 25152336
    30. Mutation of any individual cysteine of Wnt3a results in covalent Wnt oligomers through ectopic intermolecular disulfide bond formation and diminishes/abolishes Wnt signaling. PMID: 24841207
    31. epigenetic activation of Bmp2 gene expression contributes to Wnt3a-mediated direct trans-differentiation of pre-adipocytes or fibroblasts into osteoblasts PMID: 24867947
    32. Increasing amounts of soluble Frizzled8-cysteine-rich-domain protein modulated Wnt3a signaling in a biphasic manner. PMID: 24885675
    33. results demonstrate that, while ER signaling acts synergistically with Wnt3A in promoting osteogenic differentiation, Wnt3A may crosstalk with ER signaling by up-regulating ERalpha expression and down-regulating ERbeta expression in MPCs. PMID: 24340027
    34. Wnt3a/beta-catenin signaling regulates follicular development by modulating the expression of Foxo3a signaling components. PMID: 24246780
    35. Smad4 regulates maturation of skeletal collagen and osteoblast survival, and is required for matrix-forming responses to both BMP2 and Wnt3a. PMID: 24006258
    36. Jianpi Bushen Prescription plays a protective role on radiation-induced bone marrow through the activation of the Wnt3a signaling pathway. PMID: 24114209
    37. Structure-based mutational analysis of mouse Wnt3a shows that the linker between the N- and C-terminal domains is required for LRP6 binding. PMID: 23791946
    38. Results demonstrated that Wnt3a regulates proliferation, apoptosis and enhances function of pancreatic NIT-1 beta cells via activation of Wnt/beta-catenin signaling. PMID: 23296977
    39. Data indicate that Wnt/beta-catenin signaling mediates nandrolone-induced Numb transcription. PMID: 23649620
    40. WNTs have a dual and context-dependent effect on microglia acting in a homeostatic pro- and anti-inflammatory manner. PMID: 23534675
    41. We identified a number of stimuli triggering Wnt/beta-catenin pathway activation, including exogenous NKT cell activator, glycolipid alpha-GalCer, and endogenous prostaglandin E2 (PGE2). PMID: 22991247
    42. Results support a potential mechanism for the differentiation of mesenchymal stem cells into type II alveolar cells involving canonical wnt pathway activation. PMID: 23154940
    43. Wnt3a is a putative molecular partner of Zbtb20 in translating early positional information for the establishment of all developing medial pallidum fields. PMID: 22689450
    44. findings suggest that a spatially restricted Wnt signal induces an oriented cell division in embryonic stem cells that generates distinct cell fates at predictable positions relative to the Wnt source PMID: 23520113
    45. Wnt3a stimulates repaid translocation of IPMK to the cell membrane. PMID: 22940627
    46. results identify LRP8 as a novel positive factor of canonical Wnt signaling pathway and show its involvement in Wnt-induced osteoblast differentiation PMID: 22589174
    47. results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities PMID: 22761943
    48. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. PMID: 22357953
    49. Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. Wnt3a plays an important role in melanocyte homeostasis. PMID: 22710324
    50. These data suggest that stabilization of beta-catenin by WNT3A inhibits BMP2-mediated induction of cementoblast/osteoblast differentiation in SVF4 cells, although BMP2 requires endogenous Wnt/beta-catenin signaling to promote cell maturation. PMID: 22150562

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  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族:
    Wnt family
  • 組織特異性:
    Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.
  • 數據庫鏈接:

    KEGG: mmu:22416

    STRING: 10090.ENSMUSP00000010044

    UniGene: Mm.1367



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