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  4. Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit

  • 中文名稱：
    小鼠血漿蛋白酶C1抑制劑(SERPING1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL021086MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠血漿蛋白酶C1抑制劑(SERPING1)酶聯免疫試劑盒（CSB-EL021086MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPING1含量。SERPING1是一種編碼參與補體級聯調節的高度糖基化血漿蛋白的基因，其編碼的蛋白質C1抑制劑抑制激活的第一補體成分的C1r和C1s，從而調節補體激活。其缺乏與遺傳性血管神經性水腫（HANE）有關，并參與多種疾病的發生。試劑盒檢測范圍為5.6 ng/mL-360 ng/mL，靈敏度為1.4 ng/mL。適用于基礎醫學研究中探索免疫調節機制、炎癥性疾病模型建立、凝血功能相關實驗以及藥物干預效果評估等科研場景，為深入解析SERPING1在補體激活、血管通透性調節等生物學過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Serping1 ELISA Kit; C1nh ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; C1 Inh ELISA Kit; C1Inh ELISA Kit; C1 esterase inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; Serpin G1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    5.6 ng/mL-360 ng/mL
  • 靈敏度：
    1.4 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Blood Coagulation
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 100  
    Range % 95-105  
    1:2000 Average % 104  
    Range % 101-107  
    1:4000 Average % 83  
    Range % 80-86  
    1:8000 Average % 98  
    Range % 95-103  
  • 回收率：
    The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 85-92  
    EDTA plasma (n=4) 96 93-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    360 2.559 2.569 2.564 2.461  
    180 2.253 2.298 2.276 2.173  
    90 1.822 1.843 1.833 1.730  
    45 1.079 1.098 1.089 0.986  
    22.5 0.602 0.634 0.618 0.515  
    11.25 0.331 0.351 0.341 0.238  
    5.6 0.205 0.220 0.213 0.110  
    0 0.102 0.104 0.103    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. May inhibit chymotrypsin and kallikrein.
  • 基因功能參考文獻：
    1. C1INH prevented myocardial ischemia reperfusion injury in mice. PMID: 22705194
    2. Transgenic mice have been generated that express human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements; C1nh production is demonstrated in mouse brain, spleen, liver, heart, kidney, and lung. PMID: 12421980
    3. In vivo, C1 inhibitor (C1INH) reduces the number of viable bacteria in the blood and peritoneal fluid and accelerates killing of bacteria by blood neutrophils and peritoneal macrophages. PMID: 17785834
    4. C1-inhibitor limits neointimal plaque formation and inflammation. This may involve blockade of complement activation, inhibition of leukocyte recruitment, and reduced triglyceride levels PMID: 18071075
    5. In addition to the protective activities mediated via inhibition of the complement system, these studies indicate that C1INH also plays a direct role in suppression of leukocyte transmigration into reperfused tissue. PMID: 18787060

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 數據庫鏈接：


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