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Mouse Osteocalcin/Bone gla protein,OT/BGP ELISA kit

  • 中文名稱:
    小鼠骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒
  • 貨號:
    CSB-E06917m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒(CSB-E06917m)檢測血清、血漿、組織勻漿樣本中的BGLAP含量。BGLAP在骨代謝中扮演重要角色。它是骨組織特異性蛋白,參與骨礦化過程。研究聚焦于其基因表達調控、與骨細胞相互作用機制,旨在揭示其在骨發育、穩態維持及相關疾病如骨質疏松發病機理中的作用,為骨病防治提供新靶點。試劑盒檢測范圍為78 pg/ml- 5000 pg/ml,靈敏度為19.5 pg/ml。適用于科研領域中對骨代謝相關機制的探索,例如骨質疏松模型研究、骨再生藥物效果評估、骨骼發育動態監測等場景本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Bglap ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/ml - 5000 pg/ml
  • 靈敏度:
    19.5 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    94

    Range %

    85-102

    1:2

    Average %

    95

    Range %

    91-100

    1:4

    Average %

    97

    Range %

    92-104

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of mouse OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-99

    EDTA plasma (n=4)

    93

    90-100

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    1.768

    1.634

    1.701

    1.521

    2500

    1.245

    1.247

    1.246

    1.066

    1250

    0.791

    0.828

    0.809

    0.629

    625

    0.490

    0.489

    0.490

    0.309

    312

    0.381

    0.361

    0.371

    0.191

    156

    0.324

    0.313

    0.318

    0.138

    78

    0.236

    0.244

    0.240

    0.060

    0

    0.185

    0.175

    0.180

     

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 基因功能參考文獻:
    1. expression in offspring bone not changed by maternal high fat diet PMID: 25279792
    2. the uncarboxylated form of osteocalcin does not trigger Akt phosphorylation and glucose uptake by itself but promotes insulin-induced glucose uptake in myotubes, probably by up-regulating Akt signaling through ERK activation PMID: 25735975
    3. The Twist2-Cre, Osterix-Cre and osteocalcin-Cre lines to generate conditional beta1 integrin deletions, were used to investigate the role of beta1 integrins on skeletal phenotype. PMID: 25183373
    4. OCN is gamma-carboxylated by the gamma-carboxylase (GGCX) on three glutamic acid residues, a cellular process requiring reduction of vitamin K by a second enzyme, VKORC1. PMID: 25753038
    5. results showed an increase in the expression of Cbfa1 and OCN in fibroblasts and osteoblasts exposed to fluoride. PMID: 24680482
    6. Data indicate that Ca2+ binding triggered a similar conformational transition in both gamma-carboxyglutamic acid-containing protein osteocalcin (Gla-OCN) and Glu-OCN from a disordered structure to a more compact/stable form. PMID: 24896339
    7. In primary adipocytes, both carboxylated and uncarboxylated osteocalcin increased basal and insulin-stimulated glucose transport. PMID: 24554534
    8. Taken together, these findings demonstrate that SMILE negatively regulates osteocalcin via a direct interaction with RUNX2 PMID: 24389415
    9. Osteoblast-specific expression of Fra-2/AP-1 controls adiponectin and osteocalcin expression and affects metabolism. PMID: 24046454
    10. contribution of OC-OPN to fracture toughness is related to their presence at the extrafibrillar organic-mineral interfaces PMID: 24128197
    11. Insulin resistance in osteoblasts led to a decrease in circulating levels of the active form of osteocalcin, thereby decreasing insulin sensitivity in skeletal muscle. PMID: 24642469
    12. loss of osteocalcin/Gprc6a signaling has a profound effect on beta-cell mass accrual during late pancreas morphogenesis PMID: 24009262
    13. This study reveals that the skeleton via osteocalcin influences cognition and contributes to the maternal influence on fetal brain development. PMID: 24074871
    14. T3 stimulates the activation of Rho-kinase in osteoblasts, which functions as a negative regulator of T3-stimulated osteocalcin synthesis PMID: 23123502
    15. Uncarboxylated osteocalcin acts via Gprc6a to induce GLP-1 release from the gut; the stimulatory effect of uncarboxylated osteocalcin on insulin secretion is largely mediated by GLP-1. PMID: 23437377
    16. These results indicate that PP2A Calpha and its activity play a negative role in osteoblast differentiation and Osterix is a key factor responsible for regulating the expressions of Bsp and OCN during PP2A Calpha-mediated osteoblast differentiation. PMID: 23042641
    17. alphaNAC interacts with histone deacetylase corepressors to control Myogenin and Osteocalcin gene expression. PMID: 23092676
    18. Data from TR4 (steroid/thyroid hormone receptor 4) knockout mice suggest that TR4 binds to promoter region of osteocalcin gene and induces osteocalcin expression; this interaction maintains osteoblast activity during bone development/remodeling. PMID: 22676849
    19. Alterations of responses in knockout mice that suggest participation of osteocalcin in transmission of information about those sensory stimuli. PMID: 22350212
    20. BMP2 induces osteoblast differentiation through Runx2-dependent ATF6 expression, which directly regulates Oc transcription. PMID: 22102412
    21. Data show that Mkp1 KO mice displayed delayed differentiation and reduced expressions of osteocalcin (OCN) and Runx2 genes associated with osteoblast maturation and function. PMID: 21852324
    22. Both BGP overexpression and treatment with purified Bone Gla Protein (BGP) resulted in stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) in chondrocytes and vascular smooth muscle cells PMID: 21757657
    23. Foxo1 is a novel negative regulator of osteoblast-specific transcription factor Runx2 and modulates IGF1/insulin-dependent regulation of osteocalcin expression in osteoblasts. PMID: 21471200
    24. Data show that osteocalcin synthesis was significantly reduced in the stable HSP27-transfected MC3T3-E1 cells and normal human osteoblasts. PMID: 21427224
    25. Findings expand the biological importance of osteocalcin, begin to unravel its molecular mode of action, and provide the first evidence that the skeleton is an endocrine regulator of fertility. PMID: 21333348
    26. the activation of PPARgamma inhibits osteocalcin expression both by suppressing the expression of Runx2 and by interfering with the transactivation ability of Runx2 PMID: 12704187
    27. 1,25-(OH)(2)D(3)and T3 upregulated osteocalcin, depending on the stage of cell differentiation. PMID: 12798773
    28. osteocalcin fibroblast growth factor response element activation is enhanced by MINT, the Msx2 interacting nuclear matrix target PMID: 15131132
    29. Nell-1 reduced osterix-producing cells and increased bone sialoprotein, osteocalcin, and BMP-7 expression. PMID: 16936265
    30. Expression of the osteoblast-specific gene OC was examined in marrow stromal cell cultures at various time points. PMID: 17705049
    31. during osteoblast differentiation, early on, when Runx2 protein and DNA-binding are maximal, Runx2 is nearly absent from the OC promoter. Later, Runx2 is recruited to the OC promoter as Runx2 mRNA, protein, and in vitro DNA binding progressively decrease. PMID: 18821584
    32. Results show that induction of alkaline phosphatase, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells. PMID: 19162043

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Osteocalcin/matrix Gla protein family
  • 組織特異性:
    Bone.
  • 數據庫鏈接:


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