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  4. Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

  • 中文名稱：
    小鼠單核細胞趨化蛋白5(MCP-5)酶聯免疫試劑盒
  • 貨號：
    CSB-E15766m
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠單核細胞趨化蛋白5(MCP-5)酶聯免疫試劑盒（CSB-E15766m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MCP-5含量。MCP - 5是一種關鍵靶點。它在免疫調節等生理過程中發揮重要作用，可能參與炎癥反應等病理進程。目前研究聚焦于其在疾病發生發展中的機制，了解它與免疫細胞的相互作用、信號傳導通路等，為開發針對性治療方法提供依據。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為16.82 pg/mL。適用于科研場景下對小鼠炎癥相關模型、免疫調節機制或藥物干預效果的研究，例如通過檢測血清中MCP - 5水平評估炎癥程度，或分析組織勻漿中趨化因子表達變化以探究疾病發生機制，為免疫學、藥理學及病理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
  • 縮寫：
    MCP-5
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    16.82 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 82-91    
    1:2 Average % 94    
    Range % 89-98    
    1:4 Average % 107    
    Range % 103-110    
    1:8 Average % 110    
    Range % 104-114    
  • 回收率：
    The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 91-99    
    EDTA plasma (n=4) 103 98-108    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.615 2.634 2.625 2.499    
    500 2.177 2.012 2.095 1.969    
    250 1.432 1.502 1.467 1.341    
    125 0.842 0.839 0.841 0.715    
    62.5 0.531 0.529 0.530 0.404    
    31.2 0.334 0.326 0.330 0.204    
    15.6 0.246 0.251 0.249 0.123    
    0 0.125 0.127 0.126 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展：
    MCP-5是一種趨化因子，全名為單核細胞趨化蛋白5。它主要參與免疫細胞的趨化和活化過程，在炎癥、感染、腫瘤等疾病的發生和發展中發揮重要作用。最新研究發現，MCP-5參與了多種腫瘤的發生和進展，同時也是一種重要的治療靶點。
  • 功能：
    Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
  • 基因功能參考文獻：
    1. After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated. PMID: 24458145
    2. Data indicate mRNA upregulation of CCR2 and CCR2 ligands CCL2, CCL7, CCL8, and CCL12 in aortas from DOCA/salt-treated hypertension. PMID: 23033370
    3. The transforming growth factor (TGF)beta type II receptor/MCP-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601
    4. CCL12 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
  • 數據庫鏈接：

    KEGG: mmu:20293

    STRING: 10090.ENSMUSP00000000194

    UniGene: Mm.867



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