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  4. Mouse Lysosome-associated membrane glycoprotein 2(LAMP2) ELISA kit

Mouse Lysosome-associated membrane glycoprotein 2(LAMP2) ELISA kit

  • 中文名稱：
    小鼠溶酶體相關膜糖蛋白2(LAMP2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012740MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠溶酶體相關膜糖蛋白2(LAMP2)酶聯免疫試劑盒（CSB-EL012740MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的LAMP2含量。LAMP2是一種重要靶點。它在細胞內吞、自噬等過程發揮關鍵作用，參與維持細胞內環境穩定。相關研究聚焦其在腫瘤等疾病中表達變化與功能機制，探索其作為生物標志物及治療靶點的潛力，為開發針對性療法提供方向。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于基礎科研中探索溶酶體相關生理機制、構建疾病模型（如神經退行性疾病或心血管疾病）的分子變化研究，以及藥物干預下溶酶體功能評估等實驗需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Lamp2; Lamp-2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B; LGP-B; CD antigen CD107b
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LAMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 87-96
    1:2 Average % 105
    Range % 101-109
    1:4 Average % 86
    Range % 83-89
    1:8 Average % 98
    Range % 94-102
  • 回收率：
    The recovery of mouse LAMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 106 103-109
    EDTA plasma (n=4) 93 90-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.492 2.534 2.513 2.371
    1000 2.017 2.009 2.013 1.871
    500 1.297 1.204 1.251 1.109
    250 0.669 0.678 0.674 0.532
    125 0.376 0.382 0.379 0.237
    62.5 0.256 0.263 0.260 0.118
    31.25 0.192 0.187 0.190 0.048
    0 0.141 0.143 0.142 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation. Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Plays a role in lysosomal protein degradation in response to starvation. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens.
  • 基因功能參考文獻：
    1. Lysosomal p38 MAPK directly phosphorylates LAMP2A at T211 and T213, which causes its membrane accumulation and active conformational change, activating chaperone-mediated autophagy. PMID: 29176575
    2. The up-regulation of Rab11, Rab7, or RILP, but not its truncated form RILP-C33, rescued LAMP2A-defective trafficking in cystinosis, whereas dominant-negative Rab11 or Rab7 impaired LAMP2A trafficking. PMID: 28465352
    3. LAMP-2 in the central nervous system has a possible role in the degradation of the various macromolecules in lysosomes and an additional function concerning protection from oxidative stress, especially in the substantia nigra. PMID: 25998250
    4. The absence of LAMP-2 in murine brain led to inflammation and abnormal behavior, including motor deficits and impaired learning. PMID: 25637286
    5. Lamp-2 deficiency prevents high-fat diet-induced obese diabetes via enhancing energy expenditure. PMID: 26271596
    6. These results suggest that VPS35 deficiency or mutation promotes Parkinson Disease (PD) pathogenesis, and reveals a crucial pathway, VPS35-Lamp2a-alpha-synuclein, to prevent PD pathogenesis. PMID: 26203154
    7. The chaperone-mediated autophagy receptor LAMP2A is downregulated in cystinotic cells. PMID: 25586965
    8. deletion of the gene encoding LAMP-2A in T cells caused deficient in vivo responses to immunization or infection with Listeria monocytogenes. PMID: 25263126
    9. Restoration of lysosome-associated membrane protein-2 levels synergizes with partial BECLIN-1 knockdown to restore autophagosome processing and to attenuate cell death after hypoxia-reoxygenation. PMID: 22592897
    10. results suggest that LAMP-2, its luminal domain in particular, plays a critical role in endosomal cholesterol transport and that this is distinct from the chaperone-mediated autophagy function of LAMP-2 PMID: 19929948
    11. LAMP-2 has a role in lysosome biogenesis and autophagy PMID: 12221139
    12. Danon's disease resulted from a yet undescribed deletion in exon 7 of lamp-2 gene. PMID: 12398843
    13. Sequencing of LAMP-2 gene showed a novel S157X mutation in exon 4. PMID: 15792868
    14. This study describe two cases of Danon disease with different clinical presentation, in whom we identified the same exon skipping mutation c.928G>A in the LAMP-2 gene. PMID: 16217705
    15. results support that changes in the dynamic distribution of LAMP-2A into and out of discrete microdomains of the lysosomal membrane contribute to regulate CMA PMID: 16917501
    16. This study underlines the importance of LAMP-2 for the maturation of phagosomes in polymorphonuclear leukocytes. PMID: 18097049
    17. Results show that LAMP-2A organizes at the lysosomal membrane into protein complexes of different sizes. PMID: 18644871
    18. Experimental investigation of five specific genes, AP3B1, ATP6AP1, BLOC1S1, LAMP2, and RAB11A, has confirmed novel roles for these proteins in the proper initiation of macroautophagy in amino acid-starved fibroblasts. PMID: 19246570

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, autophagosome membrane. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • 蛋白家族：
    LAMP family
  • 組織特異性：
    Detected in liver and kidney (at protein level). Detected in liver and kidney.
  • 數據庫鏈接：


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