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Mouse Keratin, type I cytoskeletal 18(KRT18) ELISA kit

  • 中文名稱:
    小鼠細胞角蛋白18(KTR18)酶聯免疫試劑盒
  • 貨號:
    CSB-E17158m
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠細胞角蛋白18(KTR18)酶聯免疫試劑盒(CSB-E17158m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的KRT18含量。KRT18即角蛋白18,是細胞骨架中間絲的重要組成部分。其表達異常與多種疾病密切相關,如腫瘤發生發展。研究發現,它在細胞增殖、遷移及抗凋亡等方面發揮機制作用,有望成為疾病診斷標志物和治療靶點,具有重要科研與臨床價值。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,該產品適用于科研領域中對小鼠上皮細胞損傷、肝纖維化、腫瘤模型或炎癥性疾病的研究,例如通過檢測循環系統中KRT18水平評估細胞凋亡程度、疾病進展或藥物干預效果,為分子機制探索及生物標志物分析提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Krt18 ELISA Kit; Kerd ELISA Kit; Krt1-18 ELISA Kit; Keratin ELISA Kit; type I cytoskeletal 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Keratin D ELISA Kit; Cytokeratin-18 ELISA Kit; CK-18 ELISA Kit; Keratin-18 ELISA Kit; K18 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse KRT18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    83

    Range %

    80-86

    1:2

    Average %

    93

    Range %

    89-97

    1:4

    Average %

    84

    Range %

    81-89

    1:8

    Average %

    100

    Range %

    95-105

  • 回收率:

    The recovery of mouse KRT18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    93-99

    EDTA plasma (n=4)

    89

    84-92

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.199

    2.387

    2.293

    2.122

    5

    1.502

    1.538

    1.520

    1.349

    2.5

    0.791

    0.788

    0.790

    0.619

    1.25

    0.430

    0.468

    0.449

    0.278

    0.625

    0.302

    0.312

    0.307

    0.136

    0.312

    0.252

    0.256

    0.254

    0.083

    0.156

    0.201

    0.210

    0.206

    0.035

    0

    0.172

    0.170

    0.171

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
4.0分 - 2 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:其他 1:2

產品評價: 檢測荷瘤鼠給藥之后CK18的表達情況,這個kit的標準品第一次使用達到1.5以上,窗口比較合適;

By 劉老師

樣品類型:血漿(抗凝劑) EDTA

樣品信息:小鼠

稀釋比:其他 1:2.2

產品評價: 檢測小鼠血漿,血漿需要1:2.2倍稀釋,經過TMB反應30分鐘之后,本批次的標準品最高能反應到1.4左右,窗口還算可以;檢測出的小鼠CK18本底表達為1.7ng/ml左右

By 劉老師

靶點詳情

  • 功能:
    When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 基因功能參考文獻:
    1. K18 deficiency of hepatocytes leads to steatosis, increasing with age, and finally to SH. K18 deficiency and age promote liver tumor development in mice, frequently on the basis of chromosomal instability, resembling human HCC with stemness features. PMID: 27689336
    2. K8/K18-dependent PKCdelta- and ASMase-mediated modulation of lipid raft size can explain the more prominent FasR-mediated signaling resulting from K8/K18 loss. PMID: 27422101
    3. autoantibodies were detected in aging K18-null male mice that had a related liver phenotype but normal colon compared with K8-null mice, suggesting that the autoantibodies are linked to liver rather than colonic disease PMID: 26399787
    4. In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed PMID: 26280121
    5. The findings demonstrate the near complete loss of K8/K18 with concomitant high levels of vimentin in CT26 cells, a chemically-induced mouse colonic tumor. PMID: 25882495
    6. in contrast to its importance as luminal cell marker, CK18 is dispensable for the prostate morphogenesis but contributes to adult prostate regeneration PMID: 24672777
    7. Keratin 18 increases CFTR expression by binding to its C-terminal hydrophobic domain. PMID: 23045527
    8. Oxidative stress, Nrf2 and keratin 18 up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria PMID: 22334478
    9. Keratin 8 and keratin 18 mice on the same genetic background show similar sensitivity to DDC intoxication. PMID: 22449798
    10. These findings reveal the potential for cytokeratin 18 to be used as a diagnostic marker for early detection of hepatosplenic schistosomiasis. PMID: 21357724
    11. K18 glycosylation provides a unique protective role in epithelial injury by promoting the phosphorylation and activation of cell-survival kinases. PMID: 20729838
    12. Krt18 may be a useful immunohistochemical marker for detecting hepatocellular proliferative lesions in mice. PMID: 20035116
    13. Keratin 8 and keratin 18 play roles in regulating the shape and function of mitochondria. PMID: 19825937
    14. Data show that inhibition of extracellular signal-regulated kinase 1 and 2 (ERK1/2) activation sensitizes wild-type but not keratin 8 (K8)-null mouse hepatocytes to apoptosis, and is associated with a reduction in c-Flip protein, part of a K8/K18 complex. PMID: 15282307
    15. loss of the keratin cytoskeleton causes defects in the trophoblast giant cell layer PMID: 15819413
    16. K18 overexpression protects mice from Mallory body (MB) formation and from DDC-induced liver injury, which supports the importance of the K8-to-K18 ratio in MB formation. PMID: 17187412
    17. Study demonstrates here that, in the developing placenta of the mouse, the absence of the Mrj co-chaperone prevents proteasome degradation of keratin 18 intermediate filaments, resulting in the formation of keratin inclusion bodies. PMID: 17409114
    18. study shows that dominant mutation hK18 R89C, leads to cell type-specific lethality in mice, depending on ratio of mutant to endogenous keratins; a single, endogenous K18 allele rescued embryonic lethality but caused aggregation of keratins PMID: 17617404
    19. Keratin absence or mutation is well tolerated after pancreatic but not liver injury, whereas excessive overexpression is toxic to the pancreas but not the liver when induced under basal conditions. PMID: 18349119
    20. Over expression of K18 R89C predisposes transgenic mice to thioacetamide- but not CCl(4)-induced liver fibrosis. PMID: 18395095
    21. gene expression of krt8, krt18, and krt19 and correlation with gene expression profiles and cell differentiation are compared in human and mouse embryonic stem cells PMID: 18941637
    22. Results suggest that the K8/K18 loss induces a switch in Fas-induced death signaling, likely through a DEDD involvement. PMID: 19002587
    23. Resutls show that the appearance caspase-cleaved CK18 in plasma was found to reflect formation of the caspase-cleaved epitope in PMID: 19407366

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  • 亞細胞定位:
    Nucleus matrix. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族:
    Intermediate filament family
  • 組織特異性:
    Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues.
  • 數據庫鏈接:


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