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Mouse Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名稱:
    小鼠β干擾素(IFN-β/IFNB)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E04945m
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠β干擾素(IFN-β/IFNB)酶聯(lián)免疫試劑盒(CSB-E04945m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細胞裂解物樣本中的IFNB1含量。IFNB1即干擾素β-1,它是一種細胞因子。其背景在于具有抗病毒、抗增殖和免疫調節(jié)等多種生物學活性。研究機制方面,它可與細胞表面受體結合,激活信號轉導通路,誘導下游抗病毒蛋白產生,從而發(fā)揮對病毒感染和一些免疫相關疾病的作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,該產品可廣泛應用于基礎科研場景,如病毒感染模型中免疫應答動態(tài)監(jiān)測、基因編輯動物IFN-β表達水平驗證、免疫調節(jié)藥物體外篩選評估以及炎癥相關信號通路研究等。本品僅用于科研,不用于臨床診斷,產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名:
    Ifnb1 ELISA Kit; Ifb ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %90-103
    1:2Average %89
    Range %84-95
    1:4Average %100
    Range %95-106
    1:8Average %97
    Range %92-104
  • 回收率:
    The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8993-96
    EDTA plasma (n=4)10095-106
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.516 2.568 2.542 2.437
    5002.115 2.135 2.125 2.020
    2501.401 1.435 1.418 1.313
    1250.761 0.730 0.746 0.641
    62.50.343 0.369 0.356 0.251
    31.20.242 0.253 0.248 0.143
    15.60.179 0.172 0.176 0.071
    00.104 0.106 0.105  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 3 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:1:100

產品評價: 我使用CSB-E04945m試劑盒測小鼠血清,實驗結果如圖,數(shù)據(jù)穩(wěn)定,還會繼續(xù)回購你家的試劑盒。

By 戚老師

樣品類型:組織勻漿液 肺組織勻漿上清

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我通過使用CSB-E04945m檢測了PR8病毒感染的小鼠肺組織勻漿上清,操作簡單方便易上手,靈敏度非常高,檢測結果十分精準

By 鄧老師

樣品類型:血清

樣品信息:小鼠

稀釋比:其他 1:10

產品評價: 我用CSB-E04945m檢測小鼠血清IFN-β水平,試劑盒樣本充足,樣本和稀釋液顏色對應直觀,使用說明簡潔明了,操作容易上手,數(shù)據(jù)準確,滿意。

By 劉老師

靶點詳情

  • 功能:
    Has antiviral, antibacterial and anticancer activities.
  • 基因功能參考文獻:
    1. IFNbeta activates neuronal PI3K/Akt signalling and Akt binds to transcription factor FoxA1 that translocates to the nucleus and induces PDL1. Conversely, inhibition of PI3K/Akt, FoxA1 and PDL1 blocked neuronal ability to generate FoxA1(+)Tregs. PMID: 28436428
    2. Macrophages activated by metabolic endotoxemia infiltrated into islets and produced IFNbeta, which induced beta-cell apoptosis by increasing the expression of Xaf1. PMID: 29132171
    3. Reactive oxygen species (ROS) scavenger N-acetyl cysteine (NAC) prevented mitochondrial dysfunctions, type I IFN-stimulated transcript levels, inflammatory cell infiltrate, and muscle weakness in an experimental autoimmune myositis mouse model. Thus, these data highlight a central role of mitochondria and ROS in dermatomyositis . PMID: 28623559
    4. Nontypeable Haemophilus influenzae DNA as a Pathogen-Associated Molecular Pattern Molecules triggered I-IFN response, which was STING/TBK1/IRF3 dependent. PMID: 29421524
    5. The Lipopolysaccharide and IFN-beta-mediated increase of STAT1 mRNA and protein levels was abrogated by chelation of Zn(2+) with the membrane permeable chelator N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) in RAW 264.7 macrophages. PMID: 28965604
    6. MAVS is essential for spontaneous high basal expression of IFN-beta in cardiac myocytes and the heart. PMID: 28822807
    7. Mycobacterium smegmatis induces higher Ifnb expression in macrophages than Mycobacterium avium subspecies. PMID: 28422568
    8. Chronic presence of IFN-I in the brain microenvironment, which negatively affects cognitive function, is mediated via modulation of microglial activity. PMID: 28959042
    9. In experimental autoimmune encephalomyelitis, IFN-beta inhibited downstream inflammatory cytokines through the inhibition of PI3K/AKT/NF-kappaB axis and p38, JNK-MAPK, as well as the regulation of mTOR complexes. Moreover, IFN-beta inhibited Th17 differentiation and influenced the acetylation of the Il17a and Opn gene promoters. IFN-beta plays a role in Th17 differentiation partly through the inhibition of OPN. PMID: 29127843
    10. This review briefly discusses the dysregulation of main T cell subpopulations in CNS autoimmunity and summarized the T cell targeted effects of endogenous and exogenous IFN-beta in health and EAE/MS, with emphasis on the direct actions of IFN-beta on each T cell subset involved in the disease. PMID: 27033173
    11. Rb selectively inhibits innate IFN-beta production by enhancing deacetylation of Ifnb1 promoter, exhibiting a previous unknown non-classical role in innate immunity, which also suggests a role of Rb in the regulation of type I IFN production in inflammatory or autoimmune diseases. PMID: 27267461
    12. The effect of topical TREX1 knockdown and local interferon production on HIV transmission in human cervicovaginal explants and humanized mice, is reported. PMID: 27184854
    13. The TBK1 Y179A mutant failed to rescue type I IFN production by virally infected RAW264.7 macrophages deficient in TBK1. PMID: 28049762
    14. The data demonstrate that an atypical TLR7 signaling pathway contributes to type interferon-beta expression during Y. pestis infection and suggest that the TLR7-driven type I IFN response plays an important role in determining the outcome of plague. PMID: 28847850
    15. Long-term exposure to atmospheric particulates, PM2.5 up-regulated H3K4 and H3K9 methylation in IL-6 and IFN-beta promoter regions through down-regulating Kdm6a expression. The results suggest that short-term exposure to PM2.5 significantly enhances the survival rate of influenza A-contaminated mice, while long-term PM2.5 inhalation lowers the capacity of pulmonary macrophages to secrete IL-6 and IFN-beta. PMID: 28887033
    16. Extracellular ATP reduces the replication of VSV, Newcastle disease virus, murine leukemia virus and HSV in vivo and in vitro through the P2X7 receptor; ATP increases IFN-beta expression. Mechanistically, ATP facilitates IFN-beta secretion through P38/JNK/ATF-2 signaling pathways, which are crucial in promoting antiviral immunity. PMID: 28687662
    17. study reports that mitochondrial antiviral signaling protein (MAVS), -mediated IFN-alpha/beta production and IFNAR signaling are required for alloimmune responses to the human K1 Ag in a murine model of inflammation-induced alloimmunization PMID: 28630094
    18. study identifies a novel role for RIPK1 and RIPK3, a pair of homologous serine/threonine kinases previously implicated in the regulation of necroptosis and pathologic tissue injury, in directing IFN-beta production in macrophages PMID: 28461567
    19. results suggest that, in addition to its well-known signaling activity, IFN-beta may be directly antimicrobial and be part of a growing family of cytokines and chemokines, called kinocidins, that also have antimicrobial properties. PMID: 28411186
    20. results show that resistance to HSV-1 in the trigeminal ganglia during acute infection is conferred in part by STING and IFN-alpha/beta signaling in both bone marrow-derived and -resident cells, which coalesce to support a robust HSV-1-specific CD8(+) T cell response PMID: 27511736
    21. RIPK3 regulates type I IFN both transcriptionally, by interacting with MAVS and limiting RIPK1 interaction with MAVS, and post-transcriptionally. PMID: 28410401
    22. neddylation contributes to HSV-1-induced early phase IFN-beta production. PMID: 27593482
    23. Rubicon specifically interacts with the interferon regulatory factor (IRF) association domain (IAD) of IRF3, and this interaction leads to inhibition of the dimerization of IRF3, which negatively regulates interferon-mediated antiviral response. PMID: 28468885
    24. Translation arrest is further demonstrated to be key for anti-viral response by acting synergistically with MAVS activation to amplify TBK1 signaling and IFN-beta mRNA transcription, while GADD34-dependent protein synthesis recovery contributes to the heterogeneous expression of interferon observed in dsRNA-activated cells. PMID: 28100675
    25. The authors report that Raf kinase inhibitory protein (RKIP) is essential for TBK1 activation and type I interferon production triggered by viral infection. PMID: 27753621
    26. this study shows that paracrine type I IFN can profoundly enhance innate recognition and TLR2-dependent transcriptional responses to Francisella tularensis infection upstream of its role in inflammasome regulation in primary murine peritoneal macrophages but not murine bone marrow-derived macrophages PMID: 27231145
    27. STAT2 recruits USP18 to the type I IFN receptor subunit IFNAR2 via its constitutive membrane-distal STAT2-binding site. PMID: 28165510
    28. In the exorbital lachrymal gland, the mRNA expression of IFN beta and IFN alpha (type I IFNs) was weak- to strong-positive at 1 days post inoculation (DPI), and became negative at 2DPI. PMID: 27079771
    29. TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon-beta have been identified. PMID: 28159912
    30. The authors conclude that IFN-alphabeta is pathogenic during chronic Mycobacterium africanum infection and that the pathogenic effects may be mediated through poorer control of bacterial growth. PMID: 27803172
    31. IRF-1 and interferon-alpha with interferon-beta cooperate to control acute gammaherpesvirus infection. PMID: 27795415
    32. this study shows that endosomal signalling by Lactobacillus acidophilus that leads to IFN-beta production is inhibited by TLR stimulation from the plasma membrane PMID: 27441725
    33. Truncated rabies virus phosphoprotein isoforms promote viral replication in muscle cells through their IFN-beta antagonist activities and thereby support infection of peripheral nerves. PMID: 27384657
    34. identify iNOS/NO as an integral component of IFN-beta production in response to dsRNA in hepatocytes in a pathway that involves the coordinated activities of TLR3/Trif and PKR PMID: 27226571
    35. Data show that using adipose-derived mesenchymal stem cells (AD-MSCs) expressing interferon-beta (IFN-beta) as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in experimental autoimmune encephalomyelitis (EAE) may improve the valuable effects of IFN-beta in the autoimmune disease. PMID: 27373971
    36. NLRX1 was observed to reduce interferon beta and cytokines in response to HIV-1 reverse-transcribed DNA. PMID: 27078069
    37. This study establishes IL-1beta and IFN-I levels as key homeostatic variables of protective, yet tuned, immune responses against severe invasive bacterial infection. PMID: 26962946
    38. Type I IFN Does Not Promote Susceptibility to Foodborne Listeria monocytogenes PMID: 26895837
    39. Results suggest that the lack of autocrine response to interferon-betaI by the host cell may be one mechanism for maintenance of respiratory syncytial virus persistence. PMID: 26501312
    40. TRIM33 deficiency results in high expression of Ifnb1 at late stages of macrophages activation. PMID: 26592194
    41. Inhibition of PI3K or Akt abrogated the ability of Syk deficiency to enhance IFNbeta and IL-10 in Syk deficient cells PMID: 26708990
    42. IFN-beta significantly attenuated the progression of liver fibrosis, with accompanying transcriptional downregulation of the TLR4 adaptor molecule MyD88. PMID: 25715168
    43. Thus, despite their use of the same receptor, IFNbeta and IFNalpha have unique and distinguishable biologic functions, with IFNbeta being mainly responsible for promoting lymphocytic choriomeningitis virus persistence. PMID: 25974304
    44. Mice lacking Ifnb function exhibited motor and cognitive learning impairments with accompanying alpha-synuclein-containing Lewy bodies in the brain, as well as a reduction in dopaminergic neurons and defective dopamine signaling in the nigrostriatal region. PMID: 26451483
    45. Data show that interferon beta knockout IFN-beta(-/-) mice exhibit greater resistance to Salmonella typhimurium infection. PMID: 26202980
    46. results indicate that Lyn plays a positive regulatory role in RIG-I-mediated interferon expression as a downstream component of IPS-1 PMID: 25585356
    47. Data show that decreased type I interferon gene expression in T cell subsets of human CASP8 and FADD-like apoptosis regulating protein c-FLIPS transgenic female mice. PMID: 24816846
    48. Data suggest that Brd4 (bromodomain containing 4) is essential for Toll-like receptor-stimulated interferon-beta (Ifnb) gene transcription by permitting transcription factors to interact with the Ifnb promoter in macrophages. PMID: 25891802
    49. PP1 directly interacts with IRF3 and dephosphorylates IRF3 at Ser385 and Ser396, resulting in the suppression of TLR- and RLR-triggered IFN-beta production. PMID: 25239187
    50. Interferons alpha and beta control propagation of long interspersed element-1. PMID: 25716322

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Alpha/beta interferon family
  • 數(shù)據(jù)庫鏈接:

    KEGG: mmu:15977

    STRING: 10090.ENSMUSP00000056720

    UniGene: Mm.1245



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