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Mouse Interferon γ ,IFN-γ ELISA Kit

  • 中文名稱:
    小鼠γ干擾素(IFN-γ)酶聯免疫試劑盒
  • 貨號:
    CSB-E04578m-IS
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠γ干擾素(IFN-γ)酶聯免疫試劑盒(CSB-E04578m-IS)為雙抗夾心法ELISA試劑盒,定量檢測樣本中的IFNG含量。干擾素-γ(IFNG)是一種II型干擾素,主要由活化的T細胞和NK細胞分泌。它具有抗病毒、免疫調節和抗腫瘤特性,通過與干擾素γ受體結合,激活JAK-STAT信號通路,調控多種基因表達,發揮其生物學功能。研究顯示,IFNG在腫瘤免疫治療中具有雙重作用,既能促進腫瘤細胞凋亡,也可能促進腫瘤逃逸。科研人員可通過檢測感染性疾病模型、自身免疫疾病模型或腫瘤微環境中的免疫細胞活性,亦可評估藥物或基因干預對Th1型免疫應答的影響。檢測結果可為免疫機制研究和生物標志物探索提供定量數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell culture supernates.
  • 檢測范圍:
    1.56 pg/ml-100 pg/ml
  • 靈敏度:
    0.39 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    89-98

    1:2

    Average %

    97

    Range %

    90-104

    1:4

    Average %

    91

    Range %

    85-96

    1:8

    Average %

    101

    Range %

    94-108

  • 回收率:

    The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    85-95

    EDTA plasma (n=4)

    97

    91-103

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    100

    3.000

    3.134

    3.067

    2.880

    50

    2.297

    2.178

    2.238

    2.051

    25

    1.259

    1.348

    1.303

    1.116

    12.5

    0.838

    0.829

    0.834

    0.647

    6.25

    0.537

    0.561

    0.549

    0.362

    3.12

    0.367

    0.353

    0.360

    0.173

    1.56

    0.285

    0.274

    0.280

    0.093

    0

    0.189

    0.185

    0.187

     

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:小鼠

稀釋比:其他 1:10

產品評價: 我用CSB-E04578m-IS檢測小鼠血清中干擾素-γ,第一次買華美的試劑盒,跟著說明書一步一步進行操作,最終做出來的結果并沒有得到想要的統計學差異,可是有了相應的趨勢,還需要進一步去做來驗證。說一下對于這個試劑盒的一些感受吧。 1.說明書寫的特別詳細,一步一步來就能做出來,比較簡單,但是試劑寫的是英文名字,大家用之前一定要確定好哪個是哪個,別著急用錯了,這個在說明書里也有對應的。 2.生物素標記抗體和辣根過氧化物酶標記親和素比較少,大家加樣時候要特別注意,特別精確!最好一開始先渦旋一下,把附壁的渦旋下來,我加的時候做了48個樣本,兩個我感覺用了2/3了,下次用時候估計得聯系這邊客服看能不能補加了 3.最后的結果里的標準曲線很不錯,做出來R方大于0.99,但是我組內差異做的有一些大,可能和洗板操作有關?得最近再去試一下,但是我六個組整體的趨勢確實是能看出來了 4.官網的一些資料確實很不錯,那個標曲處理的軟件也很專業,但是就是可能得一個一個輸入x值得到y值,這個比較頭大。大家做之前一定要多看看視頻和說明,不要悶頭直接做!!摸一摸稀釋濃度,同時做一下預實驗 希望我接下來能出好的結果!也希望大家也能有好的實驗結果!!科研人加油!

By 高老師

靶點詳情

  • 功能:
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能參考文獻:
    1. Data show that interleukin-2 inducible T cell kinase (Itk)negatively regulates the development of nTh1 cells that express interferon-gamma (IFNgamma) in a T-bet transcription factor (Tbet) independent manner. PMID: 28406139
    2. demonstrate in vivo that the conditional overexpression of Ifng in metanephric mesenchymal (MM) progenitors results in renal agenesis or hypoplasia. Cell death was observed in and around the MM region of E10.5-11.5 mutants where Ifng was constitutively expressed during early kidney development and resulted in retardation of branching morphogenesis. Expression of Sall1 was decreased in the MM of mutant kidneys. PMID: 29771971
    3. Data confirm Ifng as robust supporter of immune responses against tumors; here, mice treated with Chlorella vulgaris probiotic supposed as anti-carcinogen against mammary tumor, instead tumors in treated group exhibit more malignant phenotype and lower peri-tumoral neutrophil and macrophage-to-lymphocyte infiltration ratio compared to control mice; decline in serum Ifng levels correlated with tumor growth. PMID: 28229276
    4. In interferon gamma (IFNgamma)-deficient mice, Akkermansia muciniphila is significantly increased and restoration of IFNgamma levels reduces A. muciniphila abundance. PMID: 27841267
    5. this study shows the positive effects of Ifng on the early-stage differentiation and negative effects on the calcification of primary osteoblasts in vitro PMID: 29438885
    6. these data support the novel concept that IFN-gamma can have a detrimental role in the pathogenesis of influenza through a restriction in innate lymphoid cell group II activity PMID: 28513592
    7. IFN-gamma-iNOS axis are an essential pathway in the pathogenesis of arenavirus hemorrhagic fever. PMID: 28826838
    8. IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
    9. IRF-1 may be at the nexus of the interplay between IFN-gamma and IL-6 in exacerbating a xenobiotic-induced inflammatory response, regulation of interferon responsive genes and autoimmunity PMID: 28453771
    10. an enhanced expression of PD-L1 was observed besides an increased production of IFN-gamma by TH2 cells. PMID: 28917991
    11. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
    12. SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
    13. The complex role of IFN-gamma in autoimmunity and cholangitis.IFN-gamma induces IL-30 production which suppresses IFN-gamma mediated liver inflammation. PMID: 27721424
    14. this study shows that IFN-gamma promotes transendothelial migration of CD4(+) T cells across the blood-brain barrier PMID: 28682305
    15. Addition of a disintegrin and metallopeptidase domain 17 (ADAM17) to the culture supernatant of stimulated splenocytes decreased Interferon-gamma (IFN-gamma) concentration. PMID: 27573075
    16. a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
    17. the protective effect on metastasis was lost upon patrolling monocyte or NK cell depletion, IL15 neutralization, or IFNgamma ablation. The combined analysis of these approaches allowed us to establish a hierarchy in which patrolling monocytes, making IL15 in response to primary tumors, activate NK cells and IFNg production that then inhibit lung metastasis formation. PMID: 28811289
    18. Interferon-gamma derived from cytotoxic lymphocytes directly enhances their motility and cytotoxicity. PMID: 28569770
    19. this paper shows that interferon-gamma deficiency protects against aging-related goblet cell loss PMID: 27623073
    20. IL-15 induces the activation and survival of effector immune cells that are necessary for its antitumoral activity; but, long-term exposure to IL-15 is associated with the development of important side effects mainly mediated by IFN-gamma-producing T-cells PMID: 27356750
    21. Arid5a deficiency resulted in decreased levels of IFN-gamma under Th1 cell conditions, in which T-box expressed in T cells (T-bet) mRNA expression was inhibited. PMID: 27671645
    22. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
    23. these findings highlight critical roles for IFN-gamma and IL-27 in the mechanism of respiratory syncytial virus-induced exacerbation PMID: 29167232
    24. MyD88 signaling in myeloid and dendritic cells is dispensable for IFN-gamma-dependent control of type A F. tularensis infection. PMID: 28951422
    25. spleen-derived IFN-gamma induces generation of PD-L1(+)-suppressive neutrophils. PMID: 28974543
    26. These results suggest that IL-17A plays an important role in host survival against Toxoplasma gondii infection by protecting the host from an anaphylactic reaction via the downregulation of Toxoplasma gondii HSP70 and IFN-gamma production. PMID: 28893913
    27. Stat3 mediates the expression of iNOS to promote IFNgamma/TNFalpha-induced muscle atrophy. PMID: 28264935
    28. NFAT5 can modulate different T-cell responses depending on stress conditions and stimulatory context. PMID: 27479742
    29. we identify interferon-gamma (IFN-gamma) as the key inflammatory mediator controlling sortilin-1 levels PMID: 28742217
    30. this study shows that IFN-gamma induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense PMID: 26349661
    31. IFNG mediates experimental cerebral malaria by signaling within both the hematopoietic and nonhematopoietic compartments. PMID: 28874445
    32. identified TIM-4 as a novel marker for B effector 1 (Be1) cells that depend on IFN-gamma for their proinflammatory activity; TIM-4(+) B cells are enriched for IFN-gamma-producing proinflammatory Be1 cells that enhance immune responsiveness and can be specifically targeted with anti-TIM-4. PMID: 28848066
    33. results demonstrate that a population of Thy1.2(+) non-NK innate-like cells present in the liver expresses IFN-gamma and can confer protection against M. avium infection in immunocompromised mice PMID: 28687660
    34. Using IFN-gamma-deficient Th17 cells, study demonstrates the disease-amplifying role of Th17-derived IFN-gamma in dry eye disease pathogenesis. These results clearly demonstrate that Th17 cells mediate ocular surface autoimmunity through both IL-17A and IFN-gamma. PMID: 28637904
    35. IFNgamma is captured by phosphatidylserine (PS) on the surface of viable tumor cells both in vitro and in vivo then slowly released to drive long-term transcription of cytokine-response genes. PMID: 28575659
    36. Egr2 and 3 were essential to suppress Th1 differentiation in Th2 and Th17 conditions in vitro and also to control IFN-gamma-producing CD4 and CD8 T cells in response to virus infection PMID: 28455436
    37. intravital microscopy revealed IFNgamma-induced regression of the tumour vasculature, resulting in arrest of blood flow and subsequent collapse of tumours, similar to non-haemorrhagic necrosis in ischaemia PMID: 28445461
    38. studies identify the requirement of IFN-gamma stimulation as a mechanism for BC-CML and AML GVL resistance, whereas independence from IFN-gamma renders CP-CML more GVL sensitive, even with a lower-level alloimmune response. PMID: 28604385
    39. Results demonstrate that the influence of IFN-gamma on acute lymphoblastic leukemia progression may not be mediated by selection of nascent transformed cells but rather through a general suppressor of cytokine signaling -mediated reduction in B-cell precursor proliferation. PMID: 28295300
    40. The results indicate that interferon-gamma is critical for the initial priming and differentiation of cytotoxic T lymphocytes residing in the periphery to produce the most effect antitumor function within the eye. PMID: 26578649
    41. The authors showed the synergistic negative regulation of the pro-inflammatory cytokine interferon-gamma (IFNgamma) and beta3 integrin signaling in murine hematopoietic stem cells function by a novel definitive phenotyping of hematopoietic stem cells. PMID: 28673932
    42. We revealed that in mouse mesenchymal stem cells IFN-gamma-induced immunoregulation is mediated by early phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3, which is significantly enhanced by an extracellular signal-regulated kinase 1/2-dependent mTOR inhibition, thereby promoting pSTAT1 nuclear translocation. PMID: 27670240
    43. this study showsthat the nature of the protective memory response depends closely on the route of infection and highlights the role of IFN-gamma-and IL-17RA-mediated responses in the control of mucosal infection by Brucella PMID: 27036913
    44. Study showed that the intraperitoneal administration of the exogenous cytokines IFN-gamma (to promote M1 microglia ) and IL-4 (to promote M2 microglia) can correctly modulate the timing of the M1 to M2 ratio to affect epileptogenesis and to improve cognitive function in pilocarpine-induced status epilepticus. PMID: 27956120
    45. findings show that bystander activation of T cells through TLRs leads to a short but biologically significant burst of IFN-gamma production, which relies exclusively on newly synthetized transcripts and energy generated by mitochondrial respiration PMID: 27016606
    46. Study finds that a high proportion of intratumoral Nrp1-/- Tregs produce interferon-gamma (IFNgamma), which drives the fragility of surrounding wild-type Tregs, boosts anti-tumor immunity, and facilitates tumor clearance. PMID: 28552348
    47. results demonstrate that Th1 cell-driven responses in the intestine during chronic helminth infection potently influence upstream hematopoietic processes in the Bone Marrow via IFN-gamma. PMID: 27594558
    48. Data show that lactic acid in tumor microenvironments inhibited interferon-gamma (IFNgamma) and intert=leukin-4 (IL4) productions from NKT cells by inhibiting mammalian target of rapamycin (mTOR) signaling. PMID: 27995420
    49. These findings reveal an unexpected tolerogenic role for IFN-gamma during viral persistence in the liver, providing new mechanistic insights regarding the maintenance of systemic antigen-specific tolerance during HBV persistence. PMID: 27139489
    50. CD4+CD25+ regulatory T cells promoted IFN-gamma and IL-17 mycoplasma-specific CD4+ T cell responses in vitro and in vivo. PMID: 27175511

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Type II (or gamma) interferon family
  • 組織特異性:
    Released primarily from activated T lymphocytes.
  • 數據庫鏈接:


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