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  4. Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit

Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit

  • 中文名稱：
    小鼠整合素αM(Itgam/CD11b)酶聯免疫試劑盒
  • 貨號：
    CSB-E17417m
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠整合素αM(Itgam/CD11b)酶聯免疫試劑盒（CSB-E17417m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的Itgam/CD11b含量。Itgam/CD11b是重要的靶點。其背景在于在免疫細胞表面表達，參與免疫應答等生理過程。研究機制主要聚焦于其在炎癥、免疫相關疾病中的作用，如調節白細胞黏附、遷移等，有望為相關疾病治療方案的開發提供新方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，通過包被特異性捕獲抗體和標記檢測抗體的協同作用，實現對目標蛋白的高特異性識別，可廣泛應用于科研領域中小鼠免疫細胞功能研究、炎癥性疾病模型的分子機制探索、藥物干預實驗的效應評估等場景，特別適用于巨噬細胞、中性粒細胞等髓系細胞在感染、自身免疫病或腫瘤微環境中的Itgam/CD11b表達水平分析，為免疫調控機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Itgam ELISA Kit; Integrin alpha-M ELISA Kit; CD11 antigen-like family member B ELISA Kit; CR-3 alpha chain ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; CD antigen CD11b ELISA Kit
  • 縮寫：
    Itgam/CD11b
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Itgam/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 86
    Range % 81-90
    1:200 Average % 103
    Range % 99-108
    1:400 Average % 97
    Range % 92-101
    1:800 Average % 90
    Range % 86-94
  • 回收率：
    The recovery of mouse Itgam/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 88 84-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.603 2.527 2.565 2.376
    500 1.920 1.865 1.893 1.704
    250 1.367 1.441 1.404 1.215
    125 0.905 0.928 0.917 0.728
    62.5 0.586 0.574 0.580 0.391
    31.2 0.439 0.419 0.429 0.240
    15.6 0.291 0.304 0.298 0.109
    0 0.186 0.192 0.189 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
  • 蛋白家族：
    Integrin alpha chain family
  • 組織特異性：
    Predominantly expressed in monocytes and granulocytes. Expressed in a subset of peritoneal mast cells. Expressed in microglia (at protein level).
  • 數據庫鏈接：


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