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Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

  • 中文名稱:
    小鼠胰島素樣生長因子結合蛋白復合物酸不穩定亞基(IGFALS)酶聯免疫試劑盒
  • 貨號:
    CSB-EL011094MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠胰島素樣生長因子結合蛋白復合物酸不穩定亞基(IGFALS)酶聯免疫試劑盒(CSB-EL011094MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IGFALS含量。IGFALS即胰島素樣生長因子結合蛋白酸不穩定亞基,是胰島素樣生長因子(IGF)軸的關鍵組分,能與IGF和結合蛋白形成三元復合物,延長IGF半衰期。目前對其研究主要聚焦于在生長發育、代謝及疾病如腫瘤中的作用機制。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL,適用于探索小鼠模型中IGFALS在生長發育異常、代謝性疾病、內分泌調控及相關信號通路中的功能機制,也可用于評估藥物或實驗干預對IGF系統的影響,為相關基礎研究提供可靠的定量分析工具,滿足科研場景下對IGFALS表達水平的動態監測需求本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Igfals ELISA Kit; Albs ELISA Kit; AlsInsulin-like growth factor-binding protein complex acid labile subunit ELISA Kit; ALS ELISA Kit
  • 縮寫:
    IGFALS
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.12 ng/mL-200 ng/mL
  • 靈敏度:
    0.78 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %96
    Range %93-99
    1:200Average %84
    Range %80-87
    1:400Average %91
    Range %87-94
    1:800Average %107
    Range %104-110
  • 回收率:
    The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8581-89
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2001.985 1.901 1.943 1.838
    1001.543 1.578 1.561 1.456
    501.055 1.064 1.060 0.955
    250.657 0.648 0.653 0.548
    12.50.346 0.352 0.349 0.244
    6.250.248 0.268 0.258 0.153
    3.120.191 0.193 0.192 0.087
    00.106 0.104 0.105
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    May have an important role in regulating the access of circulating IGFs to the tissues.
  • 基因功能參考文獻:
    1. the Igfals gene has a role in skeletal response of male mice to anabolic hormone therapy PMID: 24424061
    2. A modest reduction in post-natal growth in the null ALS mice and in the ALS-deficient patients was observed PMID: 16114275
  • 亞細胞定位:
    Secreted, extracellular space.
  • 數據庫鏈接:

    KEGG: mmu:16005

    STRING: 10090.ENSMUSP00000060169

    UniGene: Mm.3135



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