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中文名稱:小鼠高密度脂蛋白膽固醇檢測試劑盒
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貨號:CSB-E12874m
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:小鼠高密度脂蛋白膽固醇檢測試劑盒(CSB-E12874m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HDL-C含量。HDL-C(高密度脂蛋白膽固醇)是一種被稱為“好”的膽固醇,有助于清除血液中的多余膽固醇,降低心臟病風險。研究顯示,高HDL-C水平與較低的冠心病風險相關,其作用機制包括促進膽固醇逆向轉運和抗氧化等。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL,適用于心血管疾病模型、肥胖或糖尿病研究中脂代謝動態監測、藥物干預效果評估等科研場景。通過檢測血清、血漿樣本可系統性評估循環系統中HDL-C水平,而組織勻漿檢測則有助于探索特定器官(如肝臟、脂肪組織)的局部脂質代謝調控機制,為代謝性疾病基礎研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:HDL-C
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:7.8 ng/mL-500 ng/mL
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靈敏度:1.95 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 88 Range % 84-92 1:2000 Average % 91 Range % 85-97 1:4000 Average % 99 Range % 95-104 1:8000 Average % 92 Range % 88-96 -
回收率:
The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 97 91-104 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 500 2.509 2.598 2.554 2.440 250 2.045 2.068 2.057 1.943 125 1.401 1.457 1.429 1.315 62.5 0.977 0.999 0.988 0.874 31.2 0.572 0.598 0.585 0.471 15.6 0.315 0.338 0.327 0.213 7.8 0.256 0.267 0.262 0.148 0 0.113 0.115 0.114 -
數據處理:
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貨期:3-5 working days
引用文獻
- Parabacteroides distasonis ameliorates insulin resistance via activation of intestinal GPR109a Y Sun,Nature communications,2023
- Phytosterols Alleviate Hyperlipidemia by Regulating Gut Microbiota and Cholesterol Metabolism in Mice WJ Lv,Oxidative medicine and cellular longevity,2023
- Involvement of pyroptosis pathway in epicardial adipose tissue-myocardium axis in experimental heart failure with preserved ejection fraction YY Xia,Biochemical and biophysical research communications,2022
- Resveratrol pre-treatment alleviated caerulein-induced acute pancreatitis in high-fat diet-feeding mice via suppressing the NF-κB proinflammatory signaling and improving the gut microbiota X Zhang,BMC complementary medicine and therapies,2022
- Disordered metabolism in mice lacking irisin Y Luo,Scientific Reports,2020
- Regulation of nicotinic acetylcholine receptor α3 subtype in adipose tissue dysfunction Bai X, et al,Prostaglandins & Other Lipid Mediators,2019
- Han.et al,Experimental and Therapeutic Medicine,2018
- Mingnuan Han.et al,Experimental and Therapeutic Medicine,2018
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