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  4. Mouse Glucose-6-phosphate isomerase(GPI) ELISA kit

Mouse Glucose-6-phosphate isomerase(GPI) ELISA kit

  • 中文名稱：
    小鼠果糖-6-磷酸異構酶(GPI)酶聯免疫試劑盒
  • 貨號：
    CSB-EL009717MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠果糖-6-磷酸異構酶(GPI)酶聯免疫試劑盒（CSB-EL009717MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GPI含量。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于基礎科研中能量代謝相關模型構建、藥物干預效應評估及分子機制探索等研究場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Gpi ELISA Kit; Gpi1Glucose-6-phosphate isomerase ELISA Kit; GPI ELISA Kit; EC 5.3.1.9 ELISA Kit; Autocrine motility factor ELISA Kit; AMF ELISA Kit; Neuroleukin ELISA Kit; NLK ELISA Kit; Phosphoglucose isomerase ELISA Kit; PGI ELISA Kit; Phosphohexose isomerase ELISA Kit; PHI ELISA Kit
  • 縮寫：
    GPI
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 90-99    
    1:2 Average % 94    
    Range % 87-98    
    1:4 Average % 100    
    Range % 94-105    
    1:8 Average % 97    
    Range % 91-102    
  • 回收率：
    The recovery of mouse GPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 89-102    
    EDTA plasma (n=4) 102 95-107    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    50 2.538 2.422 2.480 2.308    
    25 1.736 1.684 1.710 1.538    
    12.5 1.197 1.143 1.170 0.998    
    6.25 0.689 0.681 0.685 0.513    
    3.12 0.414 0.389 0.402 0.230    
    1.56 0.295 0.285 0.290 0.118    
    0.78 0.223 0.226 0.225 0.053    
    0 0.174 0.170 0.172 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
  • 基因功能參考文獻：
    1. PGI is a moonlighting protein that functions as a cytosolic enzyme involved in glycolysis and gluconeogenesis, and as cytokine through binding to its cell surface receptor. PMID: 8674049
    2. The mGPI-transgenic mice presented GPI more efficiently and showed a dramatic increase in negative selection and an inhibition of arthritis. PMID: 23840022
    3. Data show that silencing of AMF/PGI induced MET and suppressed the tumor growth and pulmonary metastasis of LM8 cells in nude mice. PMID: 20978190
    4. induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (tumor autocrine motility factor) PMID: 12054583
    5. acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils PMID: 12888574
    6. Gpi1 immunoreactivity was found in ganglion, amacrine, horizontal and bipolar cells, but not in rods, cones, pigment epithelium and Muller cells. PMID: 15182299
    7. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. PMID: 15342241
    8. crystal structure of Autocrine Motility Factor PMID: 15502335
    9. AMF expression significantly contributes to the aggressive phenotype of pancreatic cancer PMID: 15570012
    10. Results report the first high-resolution crystal structure of the inhibitor-free form and the eight types of inhibitor complexes of mouse autocrine motility factor. PMID: 16375918
    11. the receptor molecule for AMF/NLK/MF in leukemic differentiation is not gp78 PMID: 17071500

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  • 亞細胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    GPI family
  • 數據庫鏈接：

    KEGG: mmu:14751

    STRING: 10090.ENSMUSP00000049355

    UniGene: Mm.589



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