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  4. Mouse Galanin-like peptide(GALP) ELISA kit

Mouse Galanin-like peptide(GALP) ELISA kit

  • 中文名稱：
    小鼠甘丙肽樣肽(GALP)酶聯免疫試劑盒
  • 貨號：
    CSB-EL009222MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠甘丙肽樣肽(GALP)酶聯免疫試劑盒（CSB-EL009222MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GALP含量。GALP（甘丙肽樣肽）是一種神經肽，其基因編碼的蛋白質與甘丙肽受體1、2和3結合，對受體3親和力最高，涉及中樞神經系統生物過程，包括下丘腦對代謝和繁殖的調節。研究顯示，GALP與多種代謝和內分泌指標密切相關，可能在多囊卵巢綜合征（PCOS）等疾病中發揮重要作用。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，本試劑盒適用于探索其在肥胖、糖尿病等代謝性疾病模型中的表達變化，或用于神經內分泌調控機制的基礎研究，為體外定量分析GALP提供高特異性的科研工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    GalpGalanin-like peptide ELISA Kit
  • 縮寫：
    GALP
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 86-97
    1:2 Average % 104
    Range % 97-108
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 91
    Range % 87-95
  • 回收率：
    The recovery of mouse GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 83-92
    EDTA plasma (n=4) 94 87-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.421 2.536 2.479 2.288
    750 1.905 2.011 1.958 1.767
    375 1.328 1.413 1.371 1.180
    187.5 0.821 0.834 0.828 0.637
    94 0.514 0.527 0.521 0.330
    47 0.370 0.387 0.379 0.188
    23.5 0.311 0.315 0.313 0.122
    0 0.190 0.192 0.191 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing. Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2.
  • 基因功能參考文獻：
    1. this study demonstrates anti-obesity effect of intranasal administration of galanin-like peptide in obese mice PMID: 27323911
    2. we investigated whether PI3K signalling in neurones expressing GALP plays a role in the transcriptional regulation of the GALP gene PMID: 24796383
    3. results indicate the GALP ICV administration can further promote energy metabolism when administered to spontaneously exercising mice PMID: 24055807
    4. Distinct expression of alarin, a splice variant of GALP, in the adult mouse brain demonstrates potential functions in reproduction and metabolism. PMID: 21647713
    5. hypothalamic GALP gene expression is controlled by leptin signals PMID: 12746327
    6. GALP may play a role in readjusting energy balance under changing nutritional circumstances PMID: 18775887

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Galanin family
  • 組織特異性：
    Isoform 2 is found in brain, thymus and skin. Isoform 2 is found in the skin, in pericytes covering microvascular arterioles and venules on their abluminal surfaces. In larger vessels, isoform 2 is expressed in layers of smooth muscle cells. Isoform 2 is
  • 數據庫鏈接：


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