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  4. Mouse Fibulin-1(FBLN1) ELISA kit

Mouse Fibulin-1(FBLN1) ELISA kit

  • 中文名稱：
    小鼠腓骨蛋白-1(FBLN1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL008452MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠腓骨蛋白-1(FBLN1)酶聯免疫試劑盒（CSB-EL008452MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FBLN1含量。FBLN1（fibulin-1）是一種分泌性糖蛋白，主要存在于細胞外基質中，參與細胞粘附、遷移和信號傳導。其研究機制涉及與層粘連蛋白和巢蛋白的結合，以及通過鈣結合介導的血小板粘附。FBLN1的表達與多種疾病如動脈粥樣硬化、癌癥等密切相關。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于研究FBLN1在纖維化疾病模型中的動態變化、腫瘤微環境基質重塑機制、胚胎發育相關信號通路調控等科研領域，支持體外實驗中對不同樣本類型的FBLN1表達水平進行精確量化分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Fbln1Fibulin-1 ELISA kit; FIBL-1 ELISA kit; Basement-membrane protein 90 ELISA kit; BM-90 ELISA kit
  • 縮寫：
    FBLN1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %95
    Range %89-99
    1:2000Average %95
    Range %91-101
    1:4000Average %94
    Range %89-98
    1:8000Average %98
    Range %92-103
  • 回收率：
    The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-97
    EDTA plasma (n=4)9490-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.419 2.319 2.369 2.203
    1001.701 1.601 1.651 1.485
    501.022 1.002 1.012 0.846
    250.664 0.654 0.659 0.493
    12.50.448 0.425 0.437 0.271
    6.250.375 0.365 0.370 0.204
    3.120.287 0.281 0.284 0.118
    00.168 0.164 0.166 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

?Does?the?kit?contain?human?and/or?animal?by-products? What?is?the?standard?made?of?

A:
Thanks for your inquiry!Standard is NS0-derived recombinant mouse MMP7 protein
Standard of kit CSB-EL008452MO is NS0-derived recombinant mouse FBLN1 protein
The kit does not contain any human and/or animal by-products.

靶點詳情

  • 功能：
    Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.
  • 基因功能參考文獻：
    1. Fbln1c plays a key role in regulating airway remodelling and inflammation in chronic asthma. PMID: 28862768
    2. The FBLN1/FGF8 interaction may also be involved in the survival of neural crest cell population during development. PMID: 27402846
    3. the findings indicate that Fbln1 normally suppresses proximal OFT EMT preventing proximal cushion hypercellularity and blood cell accumulation. PMID: 25575930
    4. Fbln1 is a new positive modulator of the formation of membranous bone and endochondral bone in the skull PMID: 25201465
    5. fibulin-1 regulates versican-dependent events in ventricular morphogenesis by promoting ADAMTS1 cleavage of versican leading to suppression of trabecular cardiomyocyte proliferation mediated by the ErbB2-Map kinase pathway. PMID: 22183742
    6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2 PMID: 11733994
    7. structural and functional characterization of promoter PMID: 11829738
    8. binding of HPV E6 protein to fibulin-1 PMID: 12200142
    9. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
    10. A murine placental overgrowth phenotype is associated with elevated Fbln1 transcript levels. PMID: 16338003
    11. Fbln1 is required for the directed migration and survival of cranial neueral crest cells contributing to the development of pharyngeal glands, craniofacial skeleton, cranial nerves, aortic arch arteries, cardiac outflow tract and cephalic blood vessels. PMID: 18538758

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  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 組織特異性：
    Detected in most organs (brain, heart, lung, spleen, liver and kidney). Neurons are the predominant source of production in the brain. Not expressed significantly by astrocytes or microglia.
  • 數據庫鏈接：


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