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Mouse Fibronectin,FN ELISA Kit

  • 中文名稱:
    小鼠纖連蛋白(FN)酶聯免疫試劑盒
  • 貨號:
    CSB-E04552m
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠纖連蛋白(FN)酶聯免疫試劑盒(CSB-E04552m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的FN1含量。FN1,即纖連蛋白1,是一種重要的細胞外基質蛋白,參與細胞粘附、遷移等生物過程。研究顯示,FN1在骨骼疾病、代謝調節等方面發揮關鍵作用。近期研究發現,運動可通過促進骨骼肌分泌FN1,激活肝臟自噬,改善胰島素敏感性,從而促進機體健康。試劑盒檢測范圍為2.7 ng/ml- 2000 ng/ml,靈敏度為2.7 ng/ml。基礎研究中的細胞外基質功能探索、組織損傷修復模型評估或炎癥相關疾病機制研究,為體外實驗提供可靠檢測工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Fn1Fibronectin ELISA kit; FN) [Cleaved into: Anastellin] ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    2.7 ng/ml - 2000 ng/ml
  • 靈敏度:
    2.7 ng/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    97

    Range %

    94-1.5

    1:2

    Average %

    95

    Range %

    91-105

    1:4

    Average %

    101

    Range %

    95-108

    1:8

    Average %

    87

    Range %

    80-99

  • 回收率:

    The recovery of mouse FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    99

    89-105

    EDTA plasma (n=4)

    93

    90-99

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.427

    2.534

    2.480

    2.377

    666

    2.101

    2.237

    2.169

    2.066

    222

    1.706

    1.738

    1.722

    1.619

    74

    1.110

    1.133

    1.121

    1.018

    24.6

    0.697

    0.731

    0.714

    0.611

    8.2

    0.415

    0.435

    0.425

    0.322

    2.7

    0.273

    0.284

    0.278

    0.175

    0

    0.106

    0.101

    0.103

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    FN1(fibronectin-1)是一種大分子的膠原蛋白家族成員,參與許多生理和病理過程,如細胞外基質形成、胚胎發育、細胞粘附、細胞遷移、組織修復和癌癥進展等。最近的研究表明,FN1在許多疾病中發揮關鍵作用,如肝纖維化、心臟病、腎病、癌癥和炎癥性疾病等。研究人員正在研究FN1在這些疾病中的作用機制,并探索潛在的治療方法,以利用其作為潛在治療靶點的潛力。此外,研究還表明,FN1可以作為一種診斷和預測生物標記物,用于腫瘤的早期診斷和治療反應的監測。
  • 功能:
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 基因功能參考文獻:
    1. Lack of Fibronectin Extra Domain A Alternative Splicing Exacerbates Endothelial Dysfunction in Diabetes. PMID: 27897258
    2. fibronectins from different sources contribute differentially to specific aspects of angiogenesis. PMID: 28667352
    3. Factor XIII-A and TG2 regulate resorption, adipogenesis and plasma fibronectin homeostasis in bone and bone marrow. PMID: 28387755
    4. we disclosed the role of chemotaxis and apoptosis in the fibrotic microenvironment-enhanced seeding of tumor cells and identified the fibroblast-derived FN1 and SPP1 as the central mediators in these processes PMID: 27329720
    5. FN is a fundamental component of the hematopoietic tissue and that the EIIIA exon may play a key role in modulating hematopiesis in conditions of bone marrow stress or diseases. PMID: 27090359
    6. PD increased CKIP-1 and Nrf2 levels in the kidney tissues as well as improved the anti-oxidative effect and renal dysfunction of diabetic mice, which eventually reversed the up-regulation of FN and ICAM-1 PMID: 28286065
    7. fibronectin has a critical role in tissue morphogenesis and the development of tissue heterogeneities PMID: 27564551
    8. The aged stem cell niche in skeletal muscle contains reduced levels of FN, leading to detrimental consequences for the function and maintenance of muscle stem cells (MuSCs). FN is a preferred adhesion substrate for MuSCs and integrin-mediated signaling via focal adhesion kinase and the p38 MAPK pathway is strongly de-regulated in MuSCs from aged mice because of insufficient attachment to the niche. PMID: 27376579
    9. analysis of novel functions for two fibronectin isoforms and the mediating receptors in osteoblast differentiation PMID: 28325836
    10. scleraxis was required for TGFbeta-induced fibronectin expression: TGFbeta lost its ability to induce fibronectin expression following scleraxis knockdown. PMID: 27324126
    11. The results very comprehensively demonstrate distinct signaling pathways that regulate microfilament organization, lamellipodial structures, spreading, and movement of cells plated on galectin-3 as opposed to fibronectin. PMID: 27130204
    12. Thus, similarly to growth factor signaling, fibronectin matrix assembly during early development can be both autocrine and paracrine. PMID: 26845241
    13. These results suggest that the Rac1 regulation of the FN arrangement is involved in retinoic acid-induced cleft palate. PMID: 26329303
    14. Fibrillar cellular fibronectin supports platelet adhesion, activation, aggregation and procoagulant activity via the interplay of a series of receptors i.e. integrins alpha5beta1 and alphaIIbbeta3, the GPIb-V-IX complex, GPVI and Toll-like receptor 4. PMID: 26245230
    15. Fn1 synthesized by the neural crest mediated morphogenesis of the aortic arch artery and differentiation of neural crest cells into vascular smooth muscle cells by regulating Notch signaling. PMID: 26552887
    16. These findings indicate an indispensable role for Fn in chronic liver fibrosis/cirrhosis. PMID: 26553870
    17. Hypercholesterolemic mice deficient in extra domain A of fibronectin exhibit reduced cerebral thrombosis. PMID: 26508731
    18. the epithelial interaction between fibronectin and beta1 integrin is important for ameloblast differentiation and enamel formation. PMID: 25830530
    19. An (as yet unknown) integrin alpha5-dependent signal extrinsic to the pharyngeal endothelium mediates the formation of the 4th pharyngeal arch arteries through fibronectin. PMID: 26434918
    20. TLR4 signaling contributes to EDA(+)-FN-mediated exacerbation of atherosclerosis. PMID: 26427793
    21. 5-HT) inhibits Factor XIII-A-mediated plasma fibronectin matrix assembly and crosslinking in osteoblast cultures via direct competition with transamidation PMID: 25460579
    22. This study demonstrated that the EIIIA domain of aFn mediates proliferation of OPCs, but not migration. PMID: 25156142
    23. The DP-4 peptide activated Fn and enhanced cell adhesion activity PMID: 25092262
    24. demonstrate the susceptibility of FN in the extracellular matrix to proteolysis and the resulting loss of cell adhesion due to the decrease of FN activity PMID: 25724944
    25. fibronectin secretion has a role in mesothelial cells promotion of early ovarian cancer metastasis PMID: 25202979
    26. These data suggest that adhesion to vitronectin allows for the uncoupling of fibronectin fibril formation from downstream signaling events by reducing alpha5beta1 integrin activation and fibronectin fibril extension. PMID: 24509439
    27. Notch signaling is required for proper lymphatic valve formation and regulates integrin alpha9 and fibronectin EIIIA expression during valve morphogenesis PMID: 24917500
    28. Regional differences in cell proliferation and fibronectin structure were dependent on both soluble fibronectin concentration and fibronectin-collagen type I interactions. PMID: 24116223
    29. Data indicate that both fibronectin and VEGF then cooperate to stimulate blood vessel formation. PMID: 23908593
    30. Silencing fibronectin expression produced similar effects as miR-378a-5p. PMID: 24060687
    31. our data point to a potential role for the fibronectin-alpha5beta1 integrin interaction in promoting vascular remodeling during demyelinating disease PMID: 24056042
    32. Fibronectin and integrin alpha5beta1 have critical roles in mouse embryogenesis PMID: 23791818
    33. Fibronectin is essential for endogenous cardiac progenitor cell expansion and repair required for stabilization of cardiac function after myocardial infarction. PMID: 23652800
    34. Loss of fibronectin negatively affects precardiac mesoderm. PMID: 23715551
    35. Extra domain A-containing fibronectin is necessary for the development of nasal tissue fibrotic remodeling process in both murine and human allergic rhinitis. PMID: 23622001
    36. cleavage by gingipains directly affects the biological activity of both fibronectin and tenascin-C in a manner that might lead to increased cell detachment and loss during periodontal disease. PMID: 23313574
    37. FN and Wnt7a together regulate the homeostatic levels of satellite stem cells and satellite myogenic cells during regenerative myogenesis. PMID: 23290138
    38. RhoA activates NFAT by inducing a rise of [Ca(2+)]i in podocytes, which in turn contributes to fibronectin upregulation. PMID: 23389455
    39. Fetal fibronectin activates MMP-1, MMP-9, and COX-2 in mesenchymal cells and causes preterm labor in mice. PMID: 23184961
    40. Fibronectin stimulates migration through lipid raft dependent NHE-1 activation in mouse embryonic stem cells. PMID: 22683701
    41. Data indicate that beta3 integrin is required for Pyk2 cell surface localization and fibronectin accumulation. PMID: 22984613
    42. analysis of force induced fibronectin assembly and matrix remodeling in a 3D microtissue model of tissue morphogenesis PMID: 22961409
    43. Data indicate that expression of fibronectin (FN) was significantly higher in primary macrophages than in a macrophage-like cell line, RAW264.7, suggesting that abundant FN might suppress the responsiveness in the primary macrophages. PMID: 22809727
    44. Dataset suggests a possible role for fibronectin and its proteolytic fragments in venous thrombus resolution and identifies a novel N-terminal-derived 50 kDa fibronectin fragment that: contains the Vegf binding site. PMID: 22640974
    45. This work defines fibronectin as a novel antiapoptotic factor for hepatocytes after acute liver injury, but demonstrates that fibronectin is not essential for subsequent hepatocyte proliferation. PMID: 22318920
    46. By providing cell adhesive signals that can act with other self-renewal factors to maintain embryonic stem cell pluripotency, fibronectin is a necessary component of the self-renewal signaling pathway in culture. PMID: 22710062
    47. Data indicate that peptide-based molecular probes capable of selectively discriminating fibronectin (Fn) fibers under different strain states. PMID: 22529344
    48. Cellular fibronectin that contains the alternatively spliced extra domain A (EIIIA)promotes hepatic stellate cell motility but not differentiation into myofibroblasts. PMID: 22202457
    49. Data suggest that fibronectin is a critical mediator for TGF-beta-specific production balance of 2 major proteoglycans, aggrecan and versican, during chondrogenesis. PMID: 21822533
    50. Bacillus anthracis infection contribute to the formation of thrombi consisting of aggregated bacteria, vWF, shed SDC-1, fibrin, activated platelets and fibronectin. PMID: 22001909

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  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性:
    Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and ot
  • 數據庫鏈接:


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