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  4. Mouse Fibroblast growth factor 5(FGF5) ELISA kit

Mouse Fibroblast growth factor 5(FGF5) ELISA kit

  • 中文名稱：
    小鼠成纖維細胞生長因子5(FGF5)酶聯免疫試劑盒
  • 貨號：
    CSB-EL008632MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠成纖維細胞生長因子5(FGF5)酶聯免疫試劑盒（CSB-EL008632MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF5含量。FGF5是一個重要靶點。它屬于成纖維細胞生長因子家族，在毛囊發育、毛發周期調控等生理過程有重要作用。研究發現其異常表達可能與脫發等毛發問題相關，通過調控FGF5的表達水平或活性，有望為治療毛發疾病帶來新策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，可廣泛應用于毛發生長調控機制、基因功能驗證、疾病動物模型構建等基礎研究領域，也可用于評估藥物或基因干預對FGF5表達的影響，為毛發相關疾病研究提供可靠的科研工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Fgf5 ELISA kit; Fgf-5Fibroblast growth factor 5 ELISA kit; FGF-5 ELISA kit; Heparin-binding growth factor 5 ELISA kit; HBGF-5 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-103
    1:2 Average % 91
    Range % 86-99
    1:4 Average % 91
    Range % 88-94
    1:8 Average % 101
    Range % 96-106
  • 回收率：
    The recovery of mouse FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-94
    EDTA plasma (n=4) 106 101-111
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.555 2.633 2.594 2.485
    1000 2.142 2.258 2.200 2.091
    500 1.567 1.614 1.591 1.482
    250 1.033 1.102 1.068 0.959
    125 0.725 0.754 0.740 0.631
    62.5 0.471 0.445 0.458 0.349
    31.25 0.214 0.221 0.218 0.109
    0 0.105 0.112 0.109 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
  • 基因功能參考文獻：
    1. Recruited CD11b+ CD68+ Kupffer cells/Mphis may play an essential role in steatohepatitis and fibrosis in FGF5 null mice fed with a HFD. Recruitment and activation of bone marrow derived Mphis is the key factor to develop steatohepatitis from simple steatosis. PMID: 27708340
    2. It participates in the progression of hepatic fibrosis. PMID: 24521867
    3. Interaction of mutant genes Fgf5(go-Y), we, and wal changes the duration of hair growth cycles in mice PMID: 22567929
    4. A deletion of a 9.3-kb region in the Fgf5 gene showed insertion of a 498-bp early transposon element long terminal repeat. Results show that long hair mutation of moja/moja mice is caused by Fgf5 disruption mediated by retrotransposon insertion. PMID: 21512271
    5. parathyroid hormone-related protein and fibroblast growth factor-5 regulate the anagen to catagen transition by independent pathways PMID: 12713572
    6. the Fgf5(go) gene is a powerful modifier of mutant genes determining the process of alopecia. PMID: 19534432

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 數據庫鏈接：

    KEGG: mmu:14176

    STRING: 10090.ENSMUSP00000031280

    UniGene: Mm.5055



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