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Mouse Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

  • 中文名稱:
    小鼠上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯免疫試劑盒
  • 貨號:
    CSB-E08180m
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯免疫試劑盒(CSB-E08180m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CXCL5含量。CXCL5是一種趨化因子,在腫瘤微環境、炎癥反應等方面有重要作用。研究表明,它可招募特定免疫細胞,參與腫瘤血管生成、侵襲轉移等過程。以CXCL5為靶點,有望從調控其表達、阻斷相關信號通路等方面開發治療策略。試劑盒檢測范圍為62.5 pg/ml- 4000 pg/ml,靈敏度為15.6 pg/ml。適用于炎癥機制研究、疾病模型(如肺炎、關節炎或腫瘤模型)中趨化因子動態變化的分析,以及藥物干預實驗中生物標志物的定量評估。科研人員可通過該產品探究CXCL5在免疫調控、病理進程及潛在治療靶點中的功能,為相關基礎研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    62.5 pg/ml - 4000 pg/ml
  • 靈敏度:
    15.6 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    516.646

    497.903

    491.373

    484.723

    483.883

    477.090

    SD

    0.035

    0.033

    0.038

    0.041

    0.041

    0.038

    CV(%)

    7.172

    6.286

    7.350

    8.055

    8.039

    7.600

    TYPICAL DATA

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    4000

    2.88

    2.876

    2.878

    2.726

    2000

    2.239

    2.235

    2.237

    2.085

    1000

    1.263

    1.259

    1.261

    1.109

    500

    0.646

    0.642

    0.644

    0.492

    250

    0.342

    0.338

    0.340

    0.188

    125

    0.249

    0.245

    0.247

    0.095

    62.5

    0.218

    0.214

    0.216

    0.064

    0.064

    0

    0.154

    0.150

    0.152

     

     

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:20

    Average %

    88

    Range %

    83-91

    1:40

    Average %

    104

    Range %

    98-108

    1:80

    Average %

    92

    Range %

    87-96

    1:160

    Average %

    96

    Range %

    91-99

  • 回收率:

    The recovery of mouse ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    90

    84-94

    EDTA plasma (n=4)

    94

    87-97

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:細胞培養上清液

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我用CSB-E08180m檢測正常細胞上清及il-17a干預后細胞上清,標準曲線的OD值為3.3675-0.064,樣本的OD值區間為0.076-0.183,檢測過程較為清晰,結果準確。

By 心老師

靶點詳情

  • 功能:
    May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
  • 基因功能參考文獻:
    1. Identify Cxcl5 as a novel target of AHR-mediated gene expression in primary mouse keratinocytes. PMID: 28973351
    2. IL-17RA regulates CXL-1 and 5 production in the lungs during the adaptive response. PMID: 26871571
    3. STAT3 is required for maximal OSM-induced CXCL5 expression. PMID: 25692402
    4. CXCL5 has a role in neutrophil recruitment in TH17-mediated glomerulonephritis PMID: 24904089
    5. Since adaptive villus growth occurs despite impaired CXCL5 expression and enhanced angiogenesis, this suggests that the growth of new blood vessels is not needed for resection-induced mucosal surface area expansion following massive SBR. PMID: 24888846
    6. CXCL5 regulates pulmonary responses to infection and plays a central role in conferring clock control of inflammation. PMID: 25064128
    7. findings demonstrated that CXCL1 and CXCL5 are increased in circulation with onset of T2D, are produced by islets under stress, and synergistically affect islet function, suggesting that these chemokines participate in pathogenesis of T2D. PMID: 24928936
    8. TLR2-induced epithelial-derived CXCL5 is critical for polymorphonuclear leukocyte-driven destructive inflammation in pulmonary tuberculosis. PMID: 24509076
    9. data suggest that CXCL6 contributes to experimental pulmonary fibrosis, and CXCL6 inhibition might be used to reduce lung toxicity associated with bleomycin treatment. PMID: 23975892
    10. Our data suggest that the differential regulation of the chemokine CXCL5 between osteoblasts and endothelial cells upon FGF2 treatment is involved in Hematopoietic stem cell mobilization from the osteoblast niche or bone marrow to peripheral blood. PMID: 22827607
    11. CXCL5 modulated macrophage activation, increased expression of the cholesterol efflux regulatory protein ABCA1, and enhanced cholesterol efflux activity in macrophages. PMID: 23376791
    12. The omentum is the main site of early neutrophil entry into the peritoneal cavity, where the action of CXCL5 synergizes with matrix metalloproteinases MMP-2 and -9 to promote neutrophil migration during the inflammatory process. PMID: 23225890
    13. CXCL5-dependent signaling cascades are essential for the recruitment of macrophages to the lungs upon subacute second hand smoke exposure. PMID: 22362385
    14. Cxcr2, Cxcl5, and commensal bacteria have critical roles in regulation of the IL-17/G-CSF axis and neutrophil homeostasis at mucosal sites PMID: 22326959
    15. In a mouse model of lung ischemia-induced angiogenesis, LIX predominates in eliciting a pro-angiogenic phenotype among the three ELR+ CXC chemokines (KC, LIX, and MIP-2) studied. PMID: 20144627
    16. regulates chemokine scavenging and pulmonary host defense to bacterial infection PMID: 20643340
    17. The villus growth observed in resection-induced adaptation is associated with increased expression of the chemokine CXCL5 within the lamina propria. PMID: 20471049
    18. LIX is expressed in response to muscle damage in vivo. Its spatial distribution in injured muscle suggests expression by satellite cells. PMID: 12077361
    19. Cloning and genomic localization of Scyb5. PMID: 12439624
    20. The PMN transendothelial migration induced by the activated myocytes is dependent on the production and secretion of the chemokines LIX and KC by the activated myocytes. PMID: 14988231
    21. proliferative effect of LIX is specific towards the primitive hematopoietic cells PMID: 15036238
    22. GCP-2 modulates cellular immunity in part through IL-8Rbeta interactions PMID: 15356099
    23. alveolar epithelial type II (AEII) cells are the primary source of CXCL5 in the rodent lung via TLR4-dependent signaling PMID: 15778492
    24. CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8 in mouse PMID: 17197447
    25. One of the mechanisms whereby endogenous IFN-gamma mitigates the manifestations of CIA consists of inhibiting production of GCP-2, thereby limiting mobilization and infiltration of neutrophils, which are important actors in joint inflammation. PMID: 17200147
    26. unveils a proinflammatory pathway centered on kinin B1 receptor activation of CXCL5 leading to leukocyte trafficking PMID: 17878384
    27. CXCL5, is ectopically expressed in the corneal epithelial cells of Dstn(corn1) mice, and targeting of the receptor for this chemokine inhibits neutrophil recruitment PMID: 18628996
    28. Secretion of CXCL5 by white adipose tissue resident macrophages represents a link between obesity, inflammation, and insulin resistance. PMID: 19356715

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Intercrine alpha (chemokine CxC) family
  • 數據庫鏈接:

    KEGG: mmu:20311

    STRING: 10090.ENSMUSP00000031318

    UniGene: Mm.4660



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