在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit

Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit

  • 中文名稱：
    小鼠含EGF纖維蛋白細胞外基質蛋白 1(EFEMP1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL007450MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠含EGF纖維蛋白細胞外基質蛋白 1(EFEMP1)酶聯免疫試劑盒（CSB-EL007450MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的EFEMP1含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產品可為研究EFEMP1在小鼠模型中的生物學功能提供可靠工具，例如探索其在組織修復、腫瘤轉移或眼部疾病中的表達動態，亦可用于基因編輯動物模型的表型驗證及藥物干預實驗的效果評估，滿足基礎科研中對EFEMP1蛋白水平的精準定量需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Efemp1 ELISA Kit; Fbln3EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Fibulin-3 ELISA Kit; FIBL-3 ELISA Kit
  • 縮寫：
    EFEMP1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 92-100
    1:2 Average % 88
    Range % 84-92
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 104
    Range % 100-108
  • 回收率：
    The recovery of mouse EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 85-94
    EDTA plasma (n=4) 97 93-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.243 2.168 2.206 2.033
    1000 1.553 1.605 1.579 1.406
    500 1.016 1.013 1.015 0.842
    250 0.597 0.574 0.586 0.413
    125 0.388 0.412 0.400 0.227
    62.5 0.324 0.337 0.331 0.158
    31.25 0.277 0.271 0.274 0.101
    0 0.177 0.169 0.173 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

Regarding your ELISA kit CSB-EL007450MO, a customer is about to use it and they harvested their cells (for cell lysate samples) in RIPA buffer rather than PBS. Do you know if RIPA buffer would cause any negative effects with this kit, or is RIPA going to be expected to be OK?

A:
Thanks for your inquiry!
Generally speaking, RIPA will contain SDS, Triton X-100, NP-40 and other components. Customers now need to confirm the RIPA buffer formula. Pls note that it can not contain SDS (it will destroy the hydrogen bonds of the protein, which will lead to protein denaturation.);
For Triton X-100, NP-40, you need to control the low concentration range (generally not more than 1%, but it is not verified); For the entire RIPA buffer, sorry we have not tried this preparation method before, so we can only suggest you do a pretest if you want to try it.
Pls let me know if you have any further questions. Thank you.
Q:

Can you please advise on the immunogens used to raise the antibodies from this kit? Were they full-length or partial-length? And were the antibodies polyclonal or monoclonal?

A:
Thanks for your inquiry!CSB-EL007450MO
Capture antibody is rat monoclonal antibody and immunogen is NS0-derived recombinant protein,full-length.
Detection antibody is goat polyclonal antibody and immunogen is
NS0-derived recombinant protein,partial.

靶點詳情

  • 功能：
    Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
  • 基因功能參考文獻：
    1. TWEAK promotes migration and invasion in murine embryonic fibroblasts through a mechanism dependent on ERKs activation and Fibulin 3 down-regulation. PMID: 28383766
    2. Mutant fibulin-3 causes proteoglycan accumulation, reduction of MMP-2 and MMP-9, but increase of TIMP-3, and impairs diffusion across Bruch's membrane. PMID: 28622396
    3. Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560
    4. The Efemp1 knock-in mice reconstitute the most important histopathologic symptoms of both Malattia leventinese and age-related macular degeneration. PMID: 17664227
    5. fibulin-3 plays an important role in maintaining the integrity of fascia connective tissues and regulates aging. PMID: 17872905
    6. both fibulin-3 and -5 are important in maintaining pelvic organ support in mice. PMID: 19095964
    7. Data suggest a role for fibulin-3 in tissue maintenance and regeneration in the adult olfactory pathway. PMID: 19520159

    顯示更多

    收起更多

  • 亞細胞定位：
    Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibulin family
  • 數據庫鏈接：


主站蜘蛛池模板： 亚洲午夜精品一区二区| 国产成人精品亚洲精品| 国产乱人伦真实精品视频| 丰满少妇熟乱xxxxx视频| 亚洲精品国产精品国自产观看| 精品人妻一区二区三区四区| 久久九九精品国产综合喷水| 国产精品一区二区手机在线观看| 婷婷伊人五月尤物| 韩国乱码伦视频免费| 国产精品无码素人福利| 超碰cao已满18进入离开官网| 国内少妇偷人精品视频| 国产成人精品aa毛片| 春色校园综合人妻av| 精品无码中文字幕在线| 色综合久久中文字幕有码| 国产成人av 综合 亚洲| 国产日韩一区二区三免费高清| 俄罗斯大荫蒂女人毛茸茸| 人妻无码vs中文字幕久久av爆| 伊人久久精品无码二区麻豆| a一区二区三区乱码在线 | 欧洲| 亚洲aⅴ综合色区无码一区| 免费无码又爽又刺激成人| 国产熟妇乱子伦视频在线观看| 精品久久人人爽天天玩人人妻| 亚洲www啪成人一区二区| 东京热加勒比视频一区 | 欧美性猛交xxxx乱大交3| 久久人妻内射无码一区三区| 9九色桋品熟女内射| 在线看片免费人成视频在线影院| 无遮挡很爽很污很黄的网站| av免费不卡国产观看| 亚洲人成精品久久久久桥| 亚洲色欲av无码成人专区| 亚洲五月丁香综合视频| 天堂在/线资源中文在线| 国产亚洲欧美日韩精品一区二区| 人妻丰满熟妇av无码区app|