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  4. Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit

Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit

  • 中文名稱：
    小鼠E3泛素連接酶SMURF1(SMURF1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL021860MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠E3泛素連接酶SMURF1(SMURF1)酶聯免疫試劑盒（CSB-EL021860MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SMURF1含量。SMURF1即E3泛素連接酶，與細胞內多條信號通路密切相關。它參與調控細胞生長、分化及胚胎發育等生理過程。研究機制上，其主要通過泛素化修飾底物蛋白，影響蛋白穩定性和功能，在腫瘤、神經退行性疾病等研究中受關注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外培養細胞或動物模型中SMURF1蛋白表達水平的動態監測，為研究泛素化修飾機制、信號轉導調控及疾病相關分子網絡提供可靠工具，特別適合骨代謝研究、腫瘤微環境分析及藥物干預效果評估等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Smurf1 ELISA Kit; E3 ubiquitin-protein ligase SMURF1 ELISA Kit; EC 2.3.2.26 ELISA Kit; HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit; SMAD ubiquitination regulatory factor 1 ELISA Kit; SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMURF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-104
    1:2Average %92
    Range %88-95
    1:4Average %86
    Range %80-91
    1:8Average %89
    Range %83-98
  • 回收率：
    The recovery of mouse SMURF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)10194-109
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.804 2.600 2.702 2.535
    10002.403 2.371 2.387 2.220
    5001.747 1.850 1.799 1.632
    2500.972 0.975 0.974 0.807
    1250.594 0.581 0.588 0.421
    62.50.370 0.347 0.359 0.192
    31.250.256 0.245 0.251 0.084
    00.170 0.163 0.167 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
  • 基因功能參考文獻：
    1. Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis PMID: 30143635
    2. Smurf1 expression in osteoblast differentiation is regulated by CRTC2. PMID: 30449452
    3. Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
    4. Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
    5. TGF-beta inhibited osteoblastic differentiation by inducing the MAPK-ERK pathway which upregulated the expression of ubiquitin ligase SMURF1 and resulted in reduced presence of osteogenic proteins. PMID: 28322449
    6. demonstration that Smurf1 acts as a brake for integrin activation by controlling Kindlin-2 protein levels, a new mechanism that permits precise modulation of integrin-mediated cellular functions PMID: 28408404
    7. miR-140-5p and SMURF1 are key regulators of disease pathology in pulmonary arterial hypertension PMID: 27214554
    8. Smurf1 is required for selective autophagy of Mycobacterium tuberculosis and host defense against tuberculosis infection. PMID: 28017659
    9. Smurf1 regulates glucose metabolism and inhibits osteoblast differentiation. Smurf1 function requires the presence of serine 148 (S148) in Smurf1. PMID: 27052174
    10. Smurf1 mediates NGF signaling and ubiquitinates RhoA in growth cones, regulating the need for local translation and degradation. PMID: 25901863
    11. Smurf-mediated endocytosis of Patched1 is required in sonic hedgehog signal reception PMID: 24925320
    12. Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
    13. Smurf1-mediated Lys29-linked nonproteolytic polyubiquitination of axin1 negatively regulates Wnt/beta-catenin signaling. PMID: 23959799
    14. These results suggest that the JNK/AP-1 and ERK/Runx2 signaling pathways mediate TNF-alpha-dependent Smurf1 transcription. PMID: 23042144
    15. Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
    16. studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476
    17. ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619
    18. Smurf1 negatively regulates mesenchymal stem cell proliferation and differentiation by controlling JunB turnover through an ubiquitin-proteasome pathway. PMID: 20200942
    19. induction could trigger downregulation of bone morphogenetic protein signaling and cause vascular cell proliferation and remodeling in pulmonary arterial hypertension pulmonary arterial hypertension PMID: 20558834
    20. Smurf1 appears to be an important regulatory factor in osteoblast differentiation and a potential molecular target for identification of bone anabolic agents PMID: 12738770
    21. Smurf1 is required for the myogenic differentiation of C2C12 cells and plays an important regulatory role in the BMP-2-mediated osteoblast conversion PMID: 12871975
    22. Smurf1 plays a specific role in osteoblast differentiation and bone formation in vivo PMID: 14701828
    23. overexpression of Smurf1 specifically promotes Smad1 and Smad5 ubiquitination and degradation in embryonic lung epithelium PMID: 14711801
    24. Mating Smad6 and Smurf1 transgenic mice produced double-transgenic pups with more delayed endochondral ossification than Smad6 transgenic mice, suggesting that Smurf1 supports Smad6 function in vivo. PMID: 15123739
    25. Smurf1 negatively regulates osteoblast activity and response to BMP through controlling MEKK2 degradation. PMID: 15820682
    26. in chronic inflammatory disorders where TNF is increased, TNF induces the expression of ubiquitin ligase Smurf1 and promotes ubiquitination and proteasomal degradation of Smad1 and Runx2, leading to systemic bone loss PMID: 18567580
    27. the WW domains linker is important in complex assembly and in regulating activity of HECT-type E3s and CKIP-1 functions as the first auxiliary factor to enhance the activation of Smurf1. PMID: 18641638
    28. Mice mutant for Smurf1 and Smurf2 display planar cell polarity defects in the cochlea and convergence and extension movements defects that include a failure to close the neural tube. PMID: 19379695
    29. Initial identification of Smurf1 as an ubiquitin ligase that inhibits BMP signaling by targeting Smad1/5 for proteasomal destruction in cultured cells and Xenopus embryos. PMID: 10458166

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  • 亞細胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 數據庫鏈接：


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