Mouse Desmosine ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠鎖鏈素酶聯(lián)免疫試劑盒（CSB-E14196m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Desmosine含量。Desmosine是一種交聯(lián)氨基酸，主要存在于彈性蛋白中。其背景在于它是彈性纖維結(jié)構(gòu)穩(wěn)定的關(guān)鍵成分。研究機(jī)制上，常作為評(píng)估彈性蛋白降解的標(biāo)志物，通過(guò)檢測(cè)其含量變化，幫助了解與彈性蛋白異常降解相關(guān)疾病的發(fā)病進(jìn)程等。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，適用于科研場(chǎng)景下的小鼠模型研究，如評(píng)估疾病進(jìn)程中彈性蛋白代謝異常、驗(yàn)證藥物對(duì)組織修復(fù)的影響或探索衰老相關(guān)分子機(jī)制，常用于研究肺部纖維化、慢性阻塞性肺病（COPD）及皮膚老化等相關(guān)機(jī)制本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

Desmosine
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Desmosine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	85
	Range %	81-92
1:20	Average %	96
	Range %	91-103
1:40	Average %	103
	Range %	97-107
1:80	Average %	98
	Range %	92-102

回收率：

The recovery of mouse Desmosine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-96
EDTA plasma (n=4)	104	96-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.