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  4. Mouse Cramp ELISA Kit

Mouse Cramp ELISA Kit

  • 中文名稱：
    小鼠Cramp酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15061m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠Cramp酶聯(lián)免疫試劑盒（CSB-E15061m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CAMP含量。CAMP，即環(huán)磷酸腺苷，是細胞內(nèi)重要的第二信使。它廣泛參與細胞多種生理過程的信號傳導(dǎo)。其研究機制主要圍繞在激素等信號刺激下，通過激活腺苷酸環(huán)化酶生成，進而調(diào)節(jié)蛋白激酶活性，影響下游基因表達和細胞功能等。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，本產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究、藥物開發(fā)等領(lǐng)域的體外實驗，特別針對免疫相關(guān)動物模型的組織微環(huán)境分析、炎癥因子調(diào)控機制探索等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Camp ELISA Kit; Cnlp ELISA Kit; CrampCathelicidin antimicrobial peptide ELISA Kit; Cathelin-like protein ELISA Kit; CLP) [Cleaved into: Cathelin-related antimicrobial peptide ELISA Kit; Cramp)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse cramp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %94
    Range %89-98
    1:200Average %95
    Range %91-98
    1:400Average %96
    Range %90-102
    1:800Average %93
    Range %87-99
  • 回收率：
    The recovery of mouse cramp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8982-94
    EDTA plasma (n=4)10294-109
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.300 2.200 2.250 2.154
    501.505 1.405 1.455 1.359
    250.822 0.802 0.812 0.716
    12.50.445 0.435 0.440 0.344
    6.250.314 0.304 0.309 0.213
    3.120.211 0.201 0.206 0.110
    1.560.149 0.147 0.148 0.052
    00.097 0.095 0.096 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse CRAMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled CRAMP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a potent antimicrobial peptide.
  • 基因功能參考文獻：
    1. Mice treated with BMMNCs pre-incubated with CRAMP had smaller scars, enhanced cardiac recovery and less adverse remodeling. Histologically, this group had higher capillary density. Similarly, sustained CRAMP release from hydrogels enhanced the therapeutic effect of SDF-1, leading to enhanced functional recovery PMID: 29948752
    2. LL-37/CRAMP represents an important mediator of platelet activation and thrombo-inflammation. PMID: 29670076
    3. CRAMP deficiency impairs phagocytosis in cultured microglia cells after exposure to N. meningitides. PMID: 28915816
    4. Immunoblotting, qPCR, ChIP and siRNA-mediated gene knockdown studies revealed that the activation of phosphatidylinositol 3-kinase/protein kinase C zeta pathways in poly(I:C)-stimulated cells underlies Sp1 phosphorylation and recruitment to the mCRAMP promoter, leading to enhanced transcription PMID: 28343946
    5. The effect on insulin resistance found in Cramp-/- mice is solely due to leukocyte infiltration and not due to inflammatory phenotype of macrophages. Therefore we conclude that cathelicidin causes insulin resistance by the recruitment of myeloid cells into the adipose tissue. PMID: 26939624
    6. Cathelicidin is required for innate resistance to M. tuberculosis in a relevant animal model and is a key mediator in regulation of the levels of pro-inflammatory cytokines by calcium and cyclic nucleotides. PMID: 28097645
    7. Histological examination confirmed that CRAMP deficiency worsened the pancreatic inflammatory condition. These results indicate that CRAMP may be considered a novel modulatory mediator in mouse experimental AP. PMID: 27035328
    8. promotes olfactory epithelium inflammation PMID: 26346056
    9. overexpressed CRAMP in prostate tumor initially chemoattracts early myeloid progenitors to tumor microenvironment and mediates differentiation and polarization of early myeloid progenitors into protumorigenic M2 macrophages during PCa progression PMID: 26856684
    10. critical role in prevention of RSV-mediated disease postinfection exogenously applied LL-37 is protective against RSV-mediated disease in vivo. PMID: 26873992
    11. The aim of this project was to examine the functional impact of the human cathelicidin LL-37 and the mouse cathelicidin-related AMP (CRAMP) on the pathogenesis of lupus and arthritis. PMID: 25535966
    12. pancreatic beta-cells' production is controlled by short-chain fatty acids produced by the gut microbiota, and is defective in non-obese diabetic (NOD) mice PMID: 26253786
    13. Data indicate the role of cathelicidin-related antimicrobial peptide (CRAMP) as part of the innate immune defense against pathogens in bacterial CNS infections. PMID: 23969854
    14. Hypoxia-inducible factor-1alpha (HIF-1alpha), a transcription factor important for activating innate immune effectors, and the antimicrobial peptide LL-37 (CRAMP in mice) are key determinants of C. albicans colonization resistance. PMID: 26053625
    15. Specific structural motifs in syndecan-1 HS promote Staphylococcus aureus corneal infection by inhibiting neutrophil CRAMP. PMID: 25931123
    16. these findings show that the production of an antimicrobial peptide Camp by adipocytes is an important element for protection against S. aureus infection of the skin PMID: 25554785
    17. Cathelicidin-deficient (Cnlp(-/-)) mice produce much less LTB4 and TXB2 in vivo in response to TNF-alpha compared with control mice. PMID: 24736410
    18. observations indicate a nonredundant role for Fpr2 and its agonist CRAMP in DC maturation in immune responses. PMID: 24808174
    19. Expression of the antimicrobial peptide cathelicidin in the context of inflammation and in non-tumorous cells is an important factor for lung tumor growth. PMID: 23812430
    20. Citrullination alters immunomodulatory function of LL-37 essential for prevention of endotoxin-induced sepsis. PMID: 24771854
    21. visfatin enhances CAMP, hBD-2, hBD-3, and S100A7 production in human keratinocytes and their orthologs in murine imiquimod-treated psoriatic skin. PMID: 23499548
    22. These data demonstrated that Salmonella Typhimurium PhoQ can sense cationic antimicrobial peptides and CRAMP serves as a putative direct PhoPQ activation signal in the mouse intestine. PMID: 22919691
    23. suppresses osteoclastogenesis induced by LPS and flagellin PMID: 23826736
    24. Collectively, these findings indicate that cathelicidin protects against H. pylori infection and its associated gastritis in vivo. Our study also demonstrates the feasibility of using the transformed food-grade bacteria to deliver cathelicidin. PMID: 23254369
    25. this investigation revealed an indispensable role for BD3, BD4, and CRAMP in defense against F. solani-induced keratitis PMID: 23670560
    26. Neutrophil-derived cathelicidin promotes adhesion of classical monocytes. PMID: 23283724
    27. TLR9 can induce the expression of antimicrobial peptides such as CRAMP in response to bacterial DNA motifs in primary glial cells. PMID: 23141747
    28. LL-37 activates caspase-1 in murine macrophages, resulting in release of active IL-1beta & IL-18. LL-37 activation of the NLRP3 inflammasome utilizes P2X7 receptor-mediated potassium efflux. PMID: 23267025
    29. CRAMP may exert important immunomodulatory effects that regulate lung injury and Gram-negative bacterial dissemination. PMID: 22634613
    30. CRAMP promotes atherosclerosis by enhancement of the recruitment of inflammatory monocytes. PMID: 22394519
    31. The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells PMID: 22246800
    32. Cathelicidin signaling via the Toll-like receptor (TLR9) protects against colitis in mice. PMID: 21762664
    33. results suggest that mCRAMP differentially regulates B- and T-cell function and implicate mCRAMP in the regulation of adaptive immune responses. PMID: 21773974
    34. ER stress increases CAMP expression via NF-kappaB-C/EBPalpha activation, independent of VDR, illuminating a novel VDR-independent role for ER stress in stimulating innate immunity. PMID: 21832078
    35. The presence of pronounced host inflammatory infiltration in lesions and lymph nodes of Leishmania-infected animals was CAMP-dependent. PMID: 21501359
    36. LL-37 induces apoptosis in CTLs via multiple different mechanisms, initiated by the LL-37-induced leakage of granzymes from cytolytic granules PMID: 21134367
    37. Data show that cathelicidin is highly produced during experimental pulmonary tuberculosis from diverse cellular sources and could have significant participation in its pathogenesis. PMID: 20636399
    38. Data show flagellin-induced protection was partially abrogated in cathelicidin-related antimicrobial peptide-deficient mice. PMID: 20566829
    39. CRAMP, the unique antimicrobial peptide derived from cathelin in mouse inhibited all the responses coupled to P2X(7) receptors in macrophages from wild type mice. PMID: 19913495
    40. an important native component of innate host defence in mice and provide protection against necrotic skin infection caused by Group A Streptococcus (GAS). PMID: 11719807
    41. solution structure in TFE/H2O solution determined by CD and NMR spectroscopy PMID: 12081622
    42. Cathelicidins are localized in the cytoplasmic granules of murine bone marrow-derived mast cells and directly participate in the mast cell immune response. PMID: 12594247
    43. cathelicidin expression (CRAMP) in the skin is 10- to 100-fold greater in the perinatal period than adult PMID: 12612195
    44. Intracellular reactive oxygen intermediates and proteases regulate macrophage CRAMP expression and activity to impair the replication of an intracellular bacterial pathogen. PMID: 14983025
    45. cathelicidin antimicrobial peptides are expressed in murine mammary glands PMID: 15531744
    46. Mig-14 and VirK inhibit binding of CRAMP to Salmonella typhimurium and thus promote bacterial resistance to CRAMP PMID: 15661016
    47. important component of innate antimicrobial defense in the colon PMID: 15814717
    48. CRAMP functions as both a chemoattractant for phagocytic leukocytes and an enhancer of adaptive immune response. PMID: 15879124
    49. role of cathelicidin in host susceptibility to HSV infection PMID: 16630942
    50. Data describe the production and function of the cathelicidin antimicrobial peptides LL-37, its precursor hCAP-18 and its ortholog CRAMP in epithelial cells of human and mouse urinary tract, respectively. PMID: 16751768

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Cathelicidin family
  • 組織特異性：
    Expressed in testis, spleen, stomach, and intestine. Very low expression found in heart, lung and skeletal muscle. No expression in brain, kidney or liver.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:12796

    STRING: 10090.ENSMUSP00000107653

    UniGene: Mm.3834



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