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  4. Mouse Corticosteroid-binding globulin(SERPINA6) ELISA kit

Mouse Corticosteroid-binding globulin(SERPINA6) ELISA kit

  • 中文名稱：
    小鼠皮質類固醇結合球蛋白(SERPINA6)酶聯免疫試劑盒
  • 貨號：
    CSB-EL021062MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠皮質類固醇結合球蛋白(SERPINA6)酶聯免疫試劑盒（CSB-EL021062MO）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的SERPINA6含量。SERPINA6 作為一種靶點，其與多種生理過程相關。它屬于絲氨酸蛋白酶抑制劑家族。目前針對它的研究多聚焦于其在調節蛋白酶活性方面的機制，探索其在疾病發生發展如內分泌紊亂、代謝疾病等進程中的作用及潛在治療意義。試劑盒檢測范圍為0.2 μg/mL-80 μg/mL，適用于內分泌機制研究、應激相關疾病模型評估、糖皮質激素代謝動態分析等科研領域；該產品可為探究代謝綜合征、免疫紊亂或神經內分泌實驗中SERPINA6的表達變化提供有效工具，適用于小鼠體內激素運輸功能研究、藥物干預對皮質醇結合能力影響評估等基礎科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Serpina6 ELISA kit; CbgCorticosteroid-binding globulin ELISA kit; CBG ELISA kit; Serpin A6 ELISA kit; Transcortin ELISA kit
  • 縮寫：
    SERPINA6
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.2 μg/mL-80 μg/mL
  • 靈敏度：
    0.2 μg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-102
    1:2 Average % 95
    Range % 87-105
    1:4 Average % 91
    Range % 83-99
    1:8 Average % 95
    Range % 90-100
  • 回收率：
    The recovery of mouse SERPINA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 86-105
    EDTA plasma (n=4) 96 89-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average
    0 2.135 2.121 2.128
    0.2 1.528 1.498 1.513
    0.7 0.992 1.013 1.003
    2.5 0.544 0.552 0.548
    15 0.339 0.352 0.346
    80 0.160 0.157 0.159
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • 基因功能參考文獻：
    1. CBG is involved in the gender differences observed in corticosteroids circulating levels and plays a role in the local regulation of corticosteroids availability in organs like lung. PMID: 26741814
    2. the same gene encodes brain and liver corticosteroid-binding globulin PMID: 24246737
    3. Data suggest fine control interaction between plasma CBG upregulation and stress-induced corticosterone (CORT) release in mouse model of psychological stress; CBG appears to function in maintaining dynamic equilibrium between CBG-bound/-unbound CORT. PMID: 24048966
    4. Sequencing analysis showed that Cbg cDNA obtained from the mouse hypothalamus was homologous to Cbg cDNA obtained from the liver. PMID: 22996440
    5. Cbg-/- mice, unlike controls, remain insensitive to stress applied before memory retrieval. Furthermore, under stress conditions, we observed a blunted surge of corticosterone (CORT) in plasma and no free CORT rise in the hippocampus of Cbg-/-. PMID: 22930537
    6. study describes functional hyporesponsiveness and aldosterone resistance in distal colon of CBG(-/-) mice; steroid actions require an intrinsic but unknown function of CBG, which allows the sufficient supply of the hormone/s to the target tissue PMID: 20969731
    7. Critical role of plasma Cbg in providing an adequate endocrine and behavioral response to stress. PMID: 20022933
    8. These data suggest an active role for CBG in bioavailability, local delivery, and/or cellular signal transduction of glucocorticoids that extends beyond a function as a mere cargo transporter. PMID: 16980625

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 組織特異性：
    Expressed by the liver; secreted in plasma.
  • 數據庫鏈接：


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