在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Mouse Cold-inducible RNA-binding protein(CIRBP) ELISA kit

  • 中文名稱:
    小鼠冷誘導RNA結合蛋白(CIRBP)酶聯免疫試劑盒
  • 貨號:
    CSB-EL005440MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠冷誘導RNA結合蛋白(CIRBP)酶聯免疫試劑盒(CSB-EL005440MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CIRBP含量。CIRBP(冷誘導RNA結合蛋白)是一種進化上保守的RNA結合蛋白,包含RNA識別結構域(RRM)和RGG結構域。它在多種應激條件下表達變化,調控轉錄、轉錄后和翻譯過程。CIRBP在細胞應激反應、基因毒性應激、炎癥、神經可塑性、干細胞特性和癌癥發生發展中發揮重要作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,本試劑盒適用于體外檢測小鼠模型中CIRBP的動態表達變化,可廣泛應用于低溫應激模型構建、代謝性疾病機制研究、炎癥相關信號通路探索等科研領域,為研究CIRBP在生理調節或病理狀態下的功能提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Cirbp ELISA kit; CirpCold-inducible RNA-binding protein ELISA kit; A18 hnRNP ELISA kit; Glycine-rich RNA-binding protein CIRP ELISA kit
  • 縮寫:
    CIRBP
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 89
    Range % 85-94
    1:2 Average % 102
    Range % 96-107
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 94
    Range % 89-98
  • 回收率:
    The recovery of mouse CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 93-102
    EDTA plasma (n=4) 103 92-107
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.490 2.567 2.529 2.397
    500 2.021 2.123 2.072 1.940
    250 1.415 1.341 1.378 1.246
    125 0.791 0.782 0.787 0.655
    62.5 0.469 0.473 0.471 0.339
    31.25 0.289 0.271 0.280 0.148
    15.6 0.210 0.214 0.212 0.080
    0 0.131 0.132 0.132  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.
  • 基因功能參考文獻:
    1. CIRP induces lung endoplasmic reticulum stress and downstream responses to cause sepsis-associated acute lung injury. PMID: 28128330
    2. Study demonstrates CIRP-induced endothelial cell (EC)pyroptosis in the lungs of C57BL/6 mice for the first time. CIRP stimulates the assembly and activation of Nlrp3 inflammasome in EC accompanied with caspase-1 activation, IL-1beta release and induction of proinflammatory cell death pyroptosis. PMID: 27217302
    3. tissue damage induced NADPH oxidase activation and increased the release of reactive oxygen species via cold-inducible RNA-binding protein (CIRP)-TLR4-MyD88 signaling. PMID: 28492546
    4. temperature-dependent accumulation of Cirbp mRNA is controlled primarily by the regulation of splicing efficiency, defined as the fraction of Cirbp pre-mRNA processed into mature mRNA PMID: 27633015
    5. this paper shows that cold air stimulation induced MUC5AC expression in wild-type mice but not in CIRP-/-) mice. PMID: 27423012
    6. state. These data collectively suggest that a deficiency in CIRP accelerates the wound healing process PMID: 26743936
    7. These results can provide new insights into the molecular mechanisms of Cirp function. PMID: 25597958
    8. Findings suggest that CIRP could exert protective effects against oxidative stress, and that it might be a novel neuroprotective agent PMID: 25498861
    9. targeting CIRP offers potential therapeutic implications in the treatment of hepatic I/R injury. PMID: 25186836
    10. Cirp appears to play a critical carcinogenic function and its expression might be a useful biomarker for hepatocellular carcinomas risk prediction. PMID: 25611373
    11. Cirp promotes the development of intestinal inflammation and colorectal tumors through regulating apoptosis and production of TNFalpha and IL23 in inflammatory cells. PMID: 25187386
    12. alcohol exposure activates microglia to produce and secrete CIRP PMID: 24223948
    13. Extracellular CIRP is a detrimental factor in stimulating inflammation to cause neuronal damage in cerebral ischemia PMID: 24613680
    14. Down-regulated CIRP is involved in testicular injury after testicular torsion/detorsion. PMID: 24094970
    15. Depletion of Cirbp is found to increase the susceptibility of cells to the TNF-mediated inhibition of high amplitude expression of clock genes and modulates the TNF-induced cytokine response. PMID: 24337574
    16. In animal models of hemorrhage and sepsis, CIRP is upregulated in the heart and liver and released into the circulation. In macrophages under hypoxic stress, CIRP translocates from the nucleus to the cytosol and is released. PMID: 24097189
    17. Moderate hypothermia resulted in significant up-regulation of both RBM3 and CIRP mRNA in murine organotypic hippocampal slice cultures PMID: 23415676
    18. we discovered that downregulation of CIRP resulted in increased germ cell apoptosis, possibly via the activation of the p44/p42, p38 and SAPK/JNK MAPK pathways PMID: 23001445
    19. mRNAs binding with CIRP in testis were mostly associated with translation regulator activity, antioxidant activity, envelope and reproduction, including important mRNAs related to male infertility. PMID: 22819822
    20. CIRP confers robustness to circadian oscillators through regulation of CLOCK expression. PMID: 22923437
    21. Cirp functions to fine-tune the proliferation of undifferentiated spermatogonia by interacting with Dyrk1b. PMID: 22711815
    22. These data suggest that mild hypothermia protects cells from TNF-alpha-induced apoptosis, at least partly, via induction of Cirp, and that Cirp protects cells by activating the ERK pathway. PMID: 16569452
    23. These findings further reveal the diversity of mechanisms by which CIRP is regulated by environmental stresses and provide new insights into CIRP cytoplasmic function. PMID: 17967451
    24. CIRP enhanced extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation, and treatment with an MEK inhibitor decreased the proliferation caused by CIRP. PMID: 19158277
    25. Overexpression of CIRP may reduce testicular damage induced by cryptorchidism by down-regulating the levels of p53 and Fas. PMID: 19331798

    顯示更多

    收起更多

  • 亞細胞定位:
    Nucleus, nucleoplasm. Cytoplasm.
  • 組織特異性:
    Ubiquitous.
  • 數據庫鏈接:


主站蜘蛛池模板: 日本无遮挡真人祼交视频| 大桥久未无码吹潮在线观看 | 中文字幕一区二区三区乱码| 精品人妻av区| 久久99精品国产麻豆蜜芽| 国产欧洲精品自在自线官方| 7777久久久国产精品消防器材| 九九久久精品无码专区| 欧美 日韩 亚洲 在线| 久久综合九色欧美婷婷| 国产精品亚洲专区无码唯爱网| 中文字幕乱码在线播放| 亚洲大乳高潮日本专区| 日本熟妇美熟bbw| 欧美噜噜久久久xxx| 国产乱视频在线观看| 国产成人无码牲交免费视频| 亚洲狠狠做深爱婷婷影院| 国产免费无码一区二区三区| 国产av国片偷人妻麻豆| 欧美成人免费va影院高清| 欧美国产日韩a在线视频下载 | 美女露出奶头扒开尿口免费网站| 99热国产这里只有精品9| 制服丝袜亚洲欧美中文字幕| 中文字幕亚韩| 国产性猛交╳xxx乱大交| 中文字幕av无码人妻| 精品人妻人人做人人爽夜夜爽| 国产精品呻吟久久人妻无吗| 国产超碰无码最新上传| 69国产精品久久久久久人妻| 国内老熟妇对白xxxxhd| 99视频精品国产免费观看 | 又色又爽又黄的视频国内| 色两性网欧美| 麻豆精品一卡二卡三卡| av性色在线乱叫| 中文在线8资源库| 老子午夜理论影院理论| 国产亚洲精品a久久777777|