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中文名稱:小鼠冷誘導RNA結合蛋白(CIRBP)酶聯免疫試劑盒
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貨號:CSB-EL005440MO
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:小鼠冷誘導RNA結合蛋白(CIRBP)酶聯免疫試劑盒(CSB-EL005440MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CIRBP含量。CIRBP(冷誘導RNA結合蛋白)是一種進化上保守的RNA結合蛋白,包含RNA識別結構域(RRM)和RGG結構域。它在多種應激條件下表達變化,調控轉錄、轉錄后和翻譯過程。CIRBP在細胞應激反應、基因毒性應激、炎癥、神經可塑性、干細胞特性和癌癥發生發展中發揮重要作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,本試劑盒適用于體外檢測小鼠模型中CIRBP的動態表達變化,可廣泛應用于低溫應激模型構建、代謝性疾病機制研究、炎癥相關信號通路探索等科研領域,為研究CIRBP在生理調節或病理狀態下的功能提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:Cirbp ELISA kit; CirpCold-inducible RNA-binding protein ELISA kit; A18 hnRNP ELISA kit; Glycine-rich RNA-binding protein CIRP ELISA kit
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縮寫:CIRBP
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 85-94 1:2 Average % 102 Range % 96-107 1:4 Average % 92 Range % 87-98 1:8 Average % 94 Range % 89-98 -
回收率:
The recovery of mouse CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 93-102 EDTA plasma (n=4) 103 92-107 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.490 2.567 2.529 2.397 500 2.021 2.123 2.072 1.940 250 1.415 1.341 1.378 1.246 125 0.791 0.782 0.787 0.655 62.5 0.469 0.473 0.471 0.339 31.25 0.289 0.271 0.280 0.148 15.6 0.210 0.214 0.212 0.080 0 0.131 0.132 0.132 -
數據處理:
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貨期:3-5 working days
引用文獻
- CIRP increases Foxp3+ regulatory T cells and inhibits development of Th17 cells by enhancing TLR4-IL-2 signaling in the late phase of sepsis Z Wu, X Liu, W Huang, J Chen, S Li, J Chao, J Xie, L Liu, Y Yang, X Wu, H Qiu,International Immunopharmacology,2024
- Intracellular CIRP promotes liver regeneration via STAT3 signaling pathway activation after partial hepatectomy in mice T Wang, M Wang, W Liu, L Zhang,International Journal of Molecular Medicine,2025
- Blocking CIRP protects against acute pancreatitis by improving mitochondrial function and suppressing pyroptosis in acinar cells W Liu,Cell death discovery,2024
- Cold-Inducible RNA Binding Protein Impedes Breast Tumor Growth in the PyMT Murine Model for Breast Cancer DA Lujan,Biomedicines,2024
- Inhibition of the Interaction of TREM-1 and eCIRP Attenuates Inflammation and Improves Survival in Hepatic Ischemia/Reperfusion T Borjas,Shock (Augusta, Ga.),2023
- Intracellular CIRP promotes liver regeneration while extracellular CIRP induces ER stress after partial hepatectomy in mice F Tang,/,2024
- Targeting extracellular CIRP with an X-Aptamer shows therapeutic potential in acute pancreatitis W Liu,iScience,2023
- Hepatic ER stress suppresses adipose browning through ATF4-CIRP-ANGPTL3 cascade S Lv,Cell Reports,2022
- Luteolin Suppresses Sepsis-Induced Cold-Inducible RNA-Binding Protein Production and Lung Injury in Neonatal Mice Parvathy S.,Shock,2021
- Irisin reverses intestinal epithelial barrier dysfunction during intestinal injury via binding to the integrin αVβ5 receptor Bi J, et al,Journal of Cellular and Molecular Medicine,2019
- Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress Bi J, et al,Redox Biology,2018
相關產品
靶點詳情
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功能:Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.
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基因功能參考文獻:
- CIRP induces lung endoplasmic reticulum stress and downstream responses to cause sepsis-associated acute lung injury. PMID: 28128330
- Study demonstrates CIRP-induced endothelial cell (EC)pyroptosis in the lungs of C57BL/6 mice for the first time. CIRP stimulates the assembly and activation of Nlrp3 inflammasome in EC accompanied with caspase-1 activation, IL-1beta release and induction of proinflammatory cell death pyroptosis. PMID: 27217302
- tissue damage induced NADPH oxidase activation and increased the release of reactive oxygen species via cold-inducible RNA-binding protein (CIRP)-TLR4-MyD88 signaling. PMID: 28492546
- temperature-dependent accumulation of Cirbp mRNA is controlled primarily by the regulation of splicing efficiency, defined as the fraction of Cirbp pre-mRNA processed into mature mRNA PMID: 27633015
- this paper shows that cold air stimulation induced MUC5AC expression in wild-type mice but not in CIRP-/-) mice. PMID: 27423012
- state. These data collectively suggest that a deficiency in CIRP accelerates the wound healing process PMID: 26743936
- These results can provide new insights into the molecular mechanisms of Cirp function. PMID: 25597958
- Findings suggest that CIRP could exert protective effects against oxidative stress, and that it might be a novel neuroprotective agent PMID: 25498861
- targeting CIRP offers potential therapeutic implications in the treatment of hepatic I/R injury. PMID: 25186836
- Cirp appears to play a critical carcinogenic function and its expression might be a useful biomarker for hepatocellular carcinomas risk prediction. PMID: 25611373
- Cirp promotes the development of intestinal inflammation and colorectal tumors through regulating apoptosis and production of TNFalpha and IL23 in inflammatory cells. PMID: 25187386
- alcohol exposure activates microglia to produce and secrete CIRP PMID: 24223948
- Extracellular CIRP is a detrimental factor in stimulating inflammation to cause neuronal damage in cerebral ischemia PMID: 24613680
- Down-regulated CIRP is involved in testicular injury after testicular torsion/detorsion. PMID: 24094970
- Depletion of Cirbp is found to increase the susceptibility of cells to the TNF-mediated inhibition of high amplitude expression of clock genes and modulates the TNF-induced cytokine response. PMID: 24337574
- In animal models of hemorrhage and sepsis, CIRP is upregulated in the heart and liver and released into the circulation. In macrophages under hypoxic stress, CIRP translocates from the nucleus to the cytosol and is released. PMID: 24097189
- Moderate hypothermia resulted in significant up-regulation of both RBM3 and CIRP mRNA in murine organotypic hippocampal slice cultures PMID: 23415676
- we discovered that downregulation of CIRP resulted in increased germ cell apoptosis, possibly via the activation of the p44/p42, p38 and SAPK/JNK MAPK pathways PMID: 23001445
- mRNAs binding with CIRP in testis were mostly associated with translation regulator activity, antioxidant activity, envelope and reproduction, including important mRNAs related to male infertility. PMID: 22819822
- CIRP confers robustness to circadian oscillators through regulation of CLOCK expression. PMID: 22923437
- Cirp functions to fine-tune the proliferation of undifferentiated spermatogonia by interacting with Dyrk1b. PMID: 22711815
- These data suggest that mild hypothermia protects cells from TNF-alpha-induced apoptosis, at least partly, via induction of Cirp, and that Cirp protects cells by activating the ERK pathway. PMID: 16569452
- These findings further reveal the diversity of mechanisms by which CIRP is regulated by environmental stresses and provide new insights into CIRP cytoplasmic function. PMID: 17967451
- CIRP enhanced extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation, and treatment with an MEK inhibitor decreased the proliferation caused by CIRP. PMID: 19158277
- Overexpression of CIRP may reduce testicular damage induced by cryptorchidism by down-regulating the levels of p53 and Fas. PMID: 19331798
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亞細胞定位:Nucleus, nucleoplasm. Cytoplasm.
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組織特異性:Ubiquitous.
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