Mouse Ceruloplasmin,CP/CER ELISA Kit
-
中文名稱:小鼠銅藍蛋白(CP/CER)酶聯免疫試劑盒
-
貨號:CSB-E07023m
-
規格:96T/48T
-
價格:¥3800/¥2500
-
其他:
產品詳情
-
產品描述:小鼠銅藍蛋白(CP/CER)酶聯免疫試劑盒(CSB-E07023m)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CP含量。小鼠銅藍蛋白(CP/CER)是一種多銅氧化酶和抗氧化劑,主要在肝臟中合成,參與銅和鐵的代謝平衡,具有抗氧化活性。研究發現,銅藍蛋白在阿爾茨海默癥、帕金森病等神經退行性疾病及糖尿病、肥胖等代謝性疾病中發揮重要作用,其功能可能與促進神經細胞生長、改善學習記憶能力、調節脂質代謝和抗氧化應激有關。試劑盒檢測范圍為31.2 ng/mL-2000 ng/mL,適用于體外研究,可用于銅代謝機制探索、肝臟功能評估、炎癥性疾病模型建立等科研領域,支持小鼠血清(1:10稀釋)和細胞上清(直接檢測)等多種樣本類型。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
-
別名:Cp ELISA Kit; Ceruloplasmin ELISA Kit; EC 1.16.3.1 ELISA Kit; Ferroxidase ELISA Kit
-
縮寫:CP
-
Uniprot No.:
-
種屬:Mus musculus (Mouse)
-
樣本類型:serum, plasma, cell culture supernates, tissue homogenates
-
檢測范圍:31.2 ng/mL-2000 ng/mL
-
靈敏度:7.8 ng/mL
-
反應時間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領域:Cardiovascular
-
測定原理:quantitative
-
測定方法:Competitive
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CP/CER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 95 Range % 83-106 1:2000 Average % 92 Range % 83-104 1:4000 Average % 95 Range % 91-102 1:8000 Average % 93 Range % 87-99 -
回收率:
The recovery of mouse CP/CER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-99 EDTA plasma (n=4) 92 85-97 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 2000 0.103 0.105 0.104 1000 0.169 0.171 0.170 500 0.301 0.309 0.305 250 0.575 0.582 0.579 125 1.294 1.306 1.300 62.5 1.855 1.864 1.860 31.2 2.536 2.541 2.539 0 2.879 2.887 2.883 -
數據處理:
-
貨期:3-5 working days
相關產品
靶點詳情
-
功能:Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense.
-
基因功能參考文獻:
- We thus used hephaestin (Heph) and ceruloplasmin (Cp) single-knockout mice and Heph/Cp double-knockout mice to investigate the roles of multicopper ferroxidases(MCF) in pancreatic iron homeostasis. We found that both HEPH and CP were expressed in the mouse pancreas, and that ablation of either MCF had limited effect on the pancreatic iron levels. PMID: 29883959
- Hephaestin and ceruloplasmin have roles in facilitating iron metabolism in the mouse kidney PMID: 27991585
- In D-galactosamine-sensitized mice CP+Cu(II) increased the LPS-induced lethality from 54 to 100%, while administration of antibodies against MIF prevented the lethal effect. The enhancement by CP+Cu(II) of the pro-inflammatory signal of MIF is discussed PMID: 26091949
- mice with mutation of Cp and Heph, iron accumulates in glia, while neurons have low iron levels. Both neurons and glia degenerate and mice become ataxic unless given an iron chelator. PMID: 26303407
- Data (including data from studies in knockout mice) suggest that ceruloplasmin and hephaestin play distinct roles in regulation of gene expression in various regions of the brain and are involved in iron homeostasis. PMID: 25788583
- an increase in Cp expression could contribute to tissue damage in early experimental autoimmune encephalomyelitis PMID: 24615902
- Evidence supports a regulatory role of both proteins (Ceruloplasmin (CP) and beta-amyloid protein precursor (APP)) in defence against iron-induced oxidative damage after TBI, which presents as a tractable therapeutic target. PMID: 24509156
- Genetic interactions between Cp, Mon1a, and the Slc40a1 locus are involved in iron metabolism. PMID: 24121729
- ceruloplasmin should provide a protective shield against inadvertent oxidant production by myeloperoxidase during inflammation PMID: 23306200
- The mouse ceruloplasmin gene has been mapped to chromosome 3. PMID: 178338
- Data found an increase in ceruloplasmin levels in the plasma of Npc1 -/- mice compared to Npc1 +/+ mice, and this increase was statistically significant (*p < 0.05). PMID: 22526561
- Cp and Heph are necessary for iron export from the retina but are not essential for iron import into the retina. PMID: 22342521
- levels of 5HT and norepinephrine and the expression of BDNF and its receptor trkB, are significantly reduced in the hippocampus of ceruloplasmin knockout mice PMID: 22035068
- Ceruloplasmin deficiency reduces levels of iron and BDNF in the cortex and striatum of young mice and increases their vulnerability to stroke. PMID: 21949858
- Ceruloplasmin stimulation of reelin cleavage is in line with a possible role of ceruloplasmin in nervous system development. PMID: 20188154
- Reduction in Cp is a sensitive biomarker for copper deficiency. PMID: 20170749
- mainly acts to release iron from cells in the liver PMID: 12393173
- Glycosylphosphatidylinositol-anchored ceruloplasmin is required for iron efflux from cells in the central nervous system. PMID: 12743117
- Cp has a pro-aggregative action on newly differentiated neurons in vitro specific for Cp in a concentration-dependent and saturable fashion. PMID: 12946701
- The copper-containing yeast protein Fet3p can restore iron homeostasis in phlebotomized mice with a deletion of the ceruloplasmin gene. PMID: 14739215
- Ceruloplasmin ane hephaestin are critical for CNS iron homeostasis and that loss of Cp and Heph in the mouse leads to age-dependent retinal neurodegeneration. PMID: 15365174
- Iron accumulation is reflected in increased ferritin expression in ceruloplasmin deficient mice and is accompanied by cell loss. PMID: 16988052
- the concentrations of ceruloplasmin and lactoferrin in milk were increased fivefold and more than 38-fold, respectively, within 48 h after weaning PMID: 16989572
- Transcripts of ceruloplasmin, involved in iron efflux, were overexpressed in periportal areas and the result was confirmed by in situ hybridization study. PMID: 18222182
- ROS affects RNA-protein complex formation to promote Cp mRNA decay. PMID: 19019832
- Datan confirmed that mouse ceruloplasmin had ferroxidase activity but revealed an additional ferroxidase in ceruloplasmin knockout mouse plasma. PMID: 19076073
- Microglial and endothelial induction of Cp is a neuroprotective mechanism during inflammation because Cp-deficient mice exhibited increased iron accumulation and demyelination when exposed to LPS and neurovascular reactivity to pneumococcal meningitis PMID: 17375196
- To elucidate the role of ceruloplasmin in copper metabolism, the kinetics of copper absorption, transport, distribution, and excretion were examined utilizing (64)Cu in wild-type and aceruloplasminemic mice PMID: 11461924
顯示更多
收起更多
-
亞細胞定位:Secreted.
-
蛋白家族:Multicopper oxidase family
-
組織特異性:Expressed in many tissues, including liver, eye and brain.
-
數據庫鏈接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-