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Mouse Caspase 3,Casp-3 ELISA Kit

  • 中文名稱:
    小鼠胱天蛋白酶3(Casp-3)酶聯免疫試劑盒
  • 貨號:
    CSB-E08858m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠胱天蛋白酶3(Casp-3)酶聯免疫試劑盒(CSB-E08858m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CASP3含量。CASP3是一種由CASP3基因編碼的酶,屬于胱天蛋白酶家族,參與細胞凋亡過程。其研究機制涉及CASP3的激活、底物特異性、結構、激活途徑、抑制和與其他蛋白質的相互作用。CASP3在細胞凋亡中起著關鍵作用,負責染色質濃縮和DNA碎裂。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,本試劑盒通過特異性捕獲目標蛋白并基于酶標信號放大系統實現精準定量,適用于探索凋亡相關機制、評估藥物干預效果或疾病模型中Casp - 3的動態變化等科研用途。為體外研究細胞凋亡通路提供高效工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse caspase 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 93  
    Range % 87-97  
    1:4 Average % 103  
    Range % 98-107  
    1:8 Average % 90  
    Range % 84-95  
  • 回收率:
    The recovery of mouse caspase 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-105  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.637 2.552 2.595 2.437  
    10 2.175 2.106 2.141 1.983  
    5 1.464 1.524 1.494 1.336  
    2.5 0.832 0.829 0.831 0.673  
    1.25 0.543 0.524 0.534 0.376  
    0.625 0.366 0.339 0.353 0.195  
    0.312 0.269 0.285 0.277 0.119  
    0 0.159 0.157 0.158    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
    CASP3是一種半胱氨酸蛋白酶,是細胞凋亡過程中的關鍵酶。最新研究表明,CASP3在多種疾病的發生和發展中也發揮重要作用。例如,CASP3與神經系統疾病(如阿爾茨海默病和帕金森病)和心血管疾病等疾病的發病機制有關。此外,一些研究還發現CASP3在腫瘤細胞凋亡、免疫調節和炎癥等方面發揮著重要作用。
  • 功能:
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
  • 基因功能參考文獻:
    1. Pyrin signaling is dispensable for Clostridium difficile infection (CDI) associated intestinal epithelial cells death and for in vivo pathogenesis; C. difficile enterotoxins induce activation of executioner caspases 3/7 via the intrinsic apoptosis pathway indicating that caspase-3/7-mediated intestinal epithelial cells apoptosis is critical for in vivo host defense during early stages of CDI. PMID: 30451870
    2. Results show an association of PD with p53 and active caspase-3 overexpression and beta-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD. PMID: 30135418
    3. Because DFNA5-induced secondary necrosis and GSDMD-induced pyroptosis are dependent on CASP3 activation, we propose that they are forms of programmed necrosis. PMID: 28045099
    4. Results revealed an interaction between Oocyte-G1 and Caspase-3. Expression levels of Caspase-3 were upregulated in cells overexpressing Oocyte-G1 and downregulated in Oocyte-G1 knockdown cells and suggest that casp3 is activated by oocyte-G1 promoting male germ cell apoptosis. PMID: 29802994
    5. NKG2D is upregulated, and ROS and caspase-3 are inhibited by Strongylocentrotus nudus egg polysaccharide-enhanced antitumor 5-FU in mice PMID: 27385218
    6. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
    7. Caspases and their substrates are key mediators of apoptosis. PMID: 27552481
    8. SERT protects trophoblast cells against apoptotic cell death in caspase-3 independent pathway by terminating the 5-HT signaling. PMID: 28109119
    9. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
    10. Induction of apoptosis in epithelial cancer cells was accompanied by a switch from glycolysis to oxidative phosphorylation, cytosol acidification and caspase-3 activation. PMID: 28063999
    11. This provides new insight into the immune phenotype, mechanisms, and signaling pathways that operate in microglial neurotoxic activation in amyotrophic lateral sclerosis. PMID: 28336525
    12. ULK1 is a novel substrate of Caspase-3 and upregulation of ULK1 drives autophagy initiation in leukemia cells. PMID: 28381396
    13. These results indicate that the caspase-3/p120 RasGAP stress-sensing module impacts on carcinogen-induced liver cancer incidence but not sufficiently so as to affect overall survival. PMID: 28150874
    14. report that mutating the caspase-3 consensus site in the EAAT2 sequence with an aspartate to asparagine mutation PMID: 28342750
    15. melatonin could inhibit TLR4 expression in hypoxic microglia followed by suppression of caspase3 activation leading to decrease in production of proinflammatory mediators PMID: 27812200
    16. A significant emergence of GFP-expressing PCs and interneurons in symptomatic, but not non-symptomatic, SCA1 mice 2 weeks after the MSC injection. PMID: 27802273
    17. Conversely, treatment with LY294002 (a selective inhibitor of Akt1) reversed the effects of quercetin. In conclusion, these findings highlight the important role of quercetin in protecting against cognitive deficits and inhibiting neuronal apoptosis via the Akt signaling pathway. We believe that quercetin might prove to be a useful therapeutic component in treating cerebral I/R diseases in the near future. PMID: 27450812
    18. cognitive decline was significantly correlated with increased levels of caspase activity and tau truncated by caspase-3 PMID: 27220334
    19. this study shows that Le Carbone, a charcoal supplement, attenuates experimental colitis through caspase 3 dependent apoptotic pathways PMID: 27978459
    20. Cepharanthine treatment reduced mitochondrial membrane potential and upregulated the level of cleaved caspases, including caspase-9 and caspase-3/7, in a dose-dependent fashion. PMID: 27121492
    21. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. PMID: 26861667
    22. caspase-3 is essential for a subset of social behaviors, but despite similar hyper-locomotion in both sexes, only male Casp3(-/-) mice exhibited social interaction deficits, which is interesting given the male bias of autism PMID: 26783106
    23. Study identified CASP-3 mRNA as a new miRNA-155 target in lipopolysaccharides-activated macrophages suggesting that through destabilization of CASP-3, miR-155 contributes to the protective role to sustain macrophage function in inflammation response. PMID: 26574931
    24. does not significantly contribute to cardiomyocyte death induced by transient coronary occlusion PMID: 26924441
    25. expression level of miR-98, which can regulate Caspase-3, was significantly decreased. Huwe1, the host gene of miR-98, was positively associated with miR-98 expression after Silica NP exposure PMID: 26263183
    26. administration of 0.3 IU/mL FSH during ovarian cryopreservation by vitrification can maintain ovarian survival during ovarian vitrification and increases the blood supply with avascular transplantation via upregulation of Cx43, Cx37, and VEGF/VEGFR2 PMID: 26539488
    27. that caspase-3-mediated nuclear opening formation accompanied by histone release from the opening is a critical step toward chromatin condensation during erythropoiesis in mice PMID: 26954545
    28. MicroRNA-124 and microRNA-137 cooperatively control caspase-3 activity through BCL2L13 in hippocampal neural stem cells. PMID: 26207921
    29. impaired mTORC1-Homer-3 activity underlies PC susceptibility in Spinocerebellar ataxia type 1 and presents a promising therapeutic target PMID: 26748090
    30. GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
    31. The study demonstrates sublethal caspase-activation has an important role in cardiomyocyte differentiation and may have significant implications for promoting cardiac regeneration after myocardial injury involving exogenous or endogenous cell sources. PMID: 25763592
    32. Parkinson disease induction increased the expression of the apoptotic mediators p53 and active caspase-3 in gastrocnemius muscle. PMID: 25704481
    33. The results reveal that executioner caspases can modulate heart's cellularity and maturation during development, contributing novel information about caspase biology and heart development. PMID: 26121671
    34. Caspase-3 activity was significantly elevated after I/R in both postischemic groups PMID: 26009812
    35. Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. PMID: 26504199
    36. a novel cell fate determination mechanism to ensure cells undergo programed cell death through interfering with RPS3/NF-kappaB-conferred anti-apoptotic transcription by the fragment from partial p65 cleavage by activated Caspase-3 PMID: 26526615
    37. results indicate a novel role of Casp-2 in the osteoclast as a modulator of total and mitochondrial ROS and osteoclast differentiation PMID: 25796569
    38. The results of this study showed that the Casp3 gene is important for the proper function of the semicircular canals but less important for the otolith organ function. PMID: 25827332
    39. Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris. PMID: 25748204
    40. Caspase 3 cleavage of Pax7 has a role in inhibiting self-renewal of satellite cells PMID: 26372956
    41. deletion of p21Cip1/Waf1 and caspase-3 accelerates proliferation and partially rescues the differentiation defects of caspase-3 deficient hematopoietic stem cells PMID: 25286245
    42. The hepatic expression level of cleaved caspase-3 protein in the group treated concomitantly with doxorubicin and theanine was significantly lower than that in the doxorubicin treated group. PMID: 25680506
    43. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver PMID: 25575574
    44. Interleukin-1beta enhances FasL-induced caspase-3/-7 activity without increasing apoptosis in primary mouse hepatocytes PMID: 25551609
    45. Induction of cIAP2 expression upon microglia activation prevents the conversion of caspase-3 p19 subunit to p17 subunit. PMID: 25501826
    46. Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice. PMID: 25964561
    47. miR-21 is endowed with anti-apoptotic properties by suppressing the expression of PDCD4 gene and active caspase 3/8 fragments in the condition of renal IRI PMID: 25322693
    48. Results suggest that miR-378 overexpression attenuates high glucose-suppressed osteogenic differentiation through targeting CASP3 and activating the PI3K/Akt pathway. PMID: 25400823
    49. Results show that human or mouse cells exposed to ionizing radiation or other stresses can survive with persistent caspase-3 activation, which in turn promotes genetic instability and oncogenic transformation. PMID: 25866249
    50. cancer stimulates p-Stat3 in muscle, activating protein loss by stimulating caspase-3, myostatin, and the ubiquitin-proteasome system. PMID: 25787076

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  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Peptidase C14A family
  • 組織特異性:
    Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
  • 數據庫鏈接:


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