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Mouse CD9 antigen(CD9) ELISA kit

  • 中文名稱:
    小鼠CD9抗原(CD9)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL004969MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠CD9抗原(CD9)酶聯(lián)免疫試劑盒(CSB-EL004969MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CD9含量。CD9 作為一個(gè)重要靶點(diǎn),在細(xì)胞表面廣泛表達(dá)。其背景上與細(xì)胞黏附、遷移等活動(dòng)密切相關(guān)。研究機(jī)制方面,它可參與多種信號通路的調(diào)控,調(diào)節(jié)細(xì)胞間相互作用。對 CD9 的深入研究有助于探索疾病發(fā)生發(fā)展機(jī)制并開發(fā)相關(guān)治療策略。試劑盒檢測范圍為4.69 pg/mL-300 pg/mL,本產(chǎn)品適用于科研領(lǐng)域探究CD9在細(xì)胞間相互作用、腫瘤微環(huán)境調(diào)控、神經(jīng)再生等方向的功能機(jī)制,為體外模型、動(dòng)物實(shí)驗(yàn)或原代細(xì)胞研究中CD9蛋白表達(dá)動(dòng)態(tài)提供可靠檢測工具,滿足基礎(chǔ)研究中對生物標(biāo)志物的定量分析需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Cd9; CD9 antigen; CD antigen CD9
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    4.69 pg/mL-300 pg/mL
  • 靈敏度:
    1.17 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    99

    Range %

    92-105

    1:2

    Average %

    108

    Range %

    103-114

    1:4

    Average %

    97

    Range %

    92-103

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率:

    The recovery of mouse CD9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    88-97

    EDTA plasma (n=4)

    98

    91-105

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    300

    3.208

    3.134

    3.171

    3.028

    150

    2.727

    2.767

    2.747

    2.604

    75

    1.940

    1.988

    1.964

    1.821

    37.5

    1.052

    1.029

    1.040

    0.897

    18.75

    0.537

    0.521

    0.529

    0.386

    9.375

    0.286

    0.293

    0.289

    0.146

    4.69

    0.209

    0.199

    0.204

    0.061

    0

    0.145

    0.141

    0.143

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Acts as a receptor for PSG17. Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung.
  • 基因功能參考文獻(xiàn):
    1. The Cd9(-/-) and Cd9(-/-) Cd81(-/-) deletions are associated with a decreased microvilli density on the MII oocyte surface. Microvilli thickness is significantly increased whatever the deleted tetraspanin gene be. Only Cd9 deletion clearly disturbs the vesicular traffic, increasing the number of clathrin and exosome vesicles. PMID: 27879451
    2. CD9 regulates the T cell-stimulatory capacity of granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent bone marrow-derived Dendritic Cells (BMDCs), without affecting antigen presentation by fms-like tyrosine kinase 3 ligand (Flt3L)-dependent BMDCs. PMID: 28533221
    3. DPP4:CD9:TTSP as the protein complexes are necessary for early efficient MERS-coronavirus entry. PMID: 28759649
    4. Our results indicate that the suppression of cartilage degradation in CD9(-/-) could be in part related to an increase in the expression of the two main cartilage extracellular matrix proteins aggrecan and type II collagen. PMID: 27784871
    5. upregulation may play an important role in podocyte morphology, adhesion, and migration PMID: 25503725
    6. Tetraspanin CD9 and ectonucleotidase CD73 identify an osteochondroprogenitor population with elevated osteogenic properties. PMID: 25564652
    7. Data indicate that anti-inflammatory effects of statins are CD9-dependent. PMID: 24040034
    8. study demonstrates ablation of Cd9 had no detectable effect on de novo primary proatate tumour onset, but did significantly increase metastasis to the liver but not the lungs PMID: 23575960
    9. chemotaxis toward antigen is controlled in mast cells by a cross-talk among FcepsilonRI, tetraspanin CD9, transmembrane adaptor proteins NTAL and LAT, and cytoskeleton-regulatory proteins of the ERM family PMID: 23443658
    10. tetraspanin CD9 modulates molecular organization of integrins in lymphatic endothelial cells, thereby supporting several functions required for lymphangiogenesis PMID: 23223239
    11. Tetraspanin family member CD9 plays an important role in sperm-egg fusion. PMID: 22609062
    12. Results show that SSCs are the most concentrated in CD9(+)EPCAM(low/-) population and also suggest that EPCAM plays an important role in progenitor cell amplification in the mouse spermatogenic system. PMID: 21858196
    13. Our study demonstrated the importance of CD9 in wound re-epithelialization, linking this molecule directly to basement membrane formation and epidermal migration through participating in the regulation of the JNK/MMP-9 pathway. PMID: 21881583
    14. propose that sperm-egg fusion is a direct consequence of CD9 controlled sperm-egg adhesion properties. CD9 generates adhesion sites responsible for the strongest of the observed gamete interaction. PMID: 21690351
    15. knockout mice display abnormal adult angiogenesis PMID: 20592186
    16. This study characterizes CD9 in sperm development and fertilization. PMID: 20428892
    17. Data show that B16F1 lones stably overexpressing CD9 had reduced ability to form colonies in soft agar PMID: 19777564
    18. CD9 is identified as the first receptor for a murine PSG /glycoprotein 17/ PMID: 11805154
    19. role of CD9 in the strengthening of fertilin beta-mediated cell adhesion PMID: 11906941
    20. CD9 may play a role in LIF-mediated maintenance of undifferentiated ES cells. PMID: 11950938
    21. CD9 down-regulation may be a sensitive indicator of macrophage differentiation PMID: 12056820
    22. CD9 inhibits SMC migration by a stimulation of both stress fiber formation and integrin clustering, leading to a stimulation of FAK phosphorylation. PMID: 12083484
    23. the function of CD9 in sperm-egg fusion is was analyzed in CD9-deficient mouse eggs PMID: 12086470
    24. CD9 was enriched in myelinating oligodendrocytes and myelin internodes. Immunoprecipitation of CD9 from postnatal rat cerebrum coprecipitated beta1 integrin, CD81, and Tspan-2. PMID: 12420314
    25. CD9 is found with with the alpha6beta1 integrin heterodimer in parietal endoderm cells , suggesting a role for CD9 in alpha6beta1 mediated migration of parietal endoderm. PMID: 12745436
    26. CD9 is localized to paranodes and has a role in regulating paranodal junctional formation. PMID: 14715942
    27. CD9 is dispensable for B cell development and humoral immunity. PMID: 16116178
    28. During oogenesis the development of the ability of the oolemma to fuse with sperm may be regulated by synthesis of CD9 by the oocyte. PMID: 16719947
    29. Taken together, these results suggest that CD9 expressed on osteoclast lineage cells might positively regulate osteoclastogenesis via the regulation of p44/42 MAPK activity. PMID: 16808899
    30. CD9 expression by multiple myeloma cells is upregulated in vivo by close interaction of the cells with endothelial cells and CD9 is involved in transendothelial invasion PMID: 16900214
    31. CD9 was differentially expressed in uterus depending on the stage of implantation & was upregulated in ovarian steroid hormone-dependent manner, implicating multiple roles of CD9 in regulation of embryo implantation during the peri-implantation period. PMID: 17126340
    32. Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution. PMID: 17239847
    33. The expression levels of both CD9 mRNA and protein in the frozen oocytes were significantly lower than those found in the fresh oocytes. PMID: 17307168
    34. establish for the first time a role for CD9 in the tumorigenic process PMID: 17582603
    35. The fusion-facilitating activity of CD9-containing vesicles by examining the fusion of sperm to CD9(-/-) eggs with the aid of exogenous CD9-containing vesicles, is investigated. PMID: 18728192
    36. Immunoglobulin superfamily member IgSF8 (EWI-2) and CD9 in fertilisation: evidence of distinct functions for CD9 and a CD9-associated protein in mammalian sperm-egg interaction. PMID: 19210920
    37. macrophage CD9 negatively regulates LPS response at lipid-enriched membrane microdomains PMID: 19414803

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  • 亞細(xì)胞定位:
    Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, extracellular exosome.
  • 蛋白家族:
    Tetraspanin (TM4SF) family
  • 組織特異性:
    Expressed predominantly in the peripheral nervous system. Highly expressed in oocytes and blastocysts (at protein level). Expression is also observed on follicular oocytes in the ovary, whereas no expression is found on follicular cells (at protein level)
  • 數(shù)據(jù)庫鏈接:


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