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  4. Mouse C-X-C motif chemokine 14(CXCL14) ELISA kit

Mouse C-X-C motif chemokine 14(CXCL14) ELISA kit

  • 中文名稱：
    小鼠C-X-C趨化因子14（CXCL14）酶聯免疫試劑盒
  • 貨號：
    CSB-EL006244MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠C-X-C趨化因子14（CXCL14）酶聯免疫試劑盒（CSB-EL006244MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CXCL14含量。CXCL14是一種非ELR CXC趨化因子，主要對單核細胞具有趨化活性，參與癌癥、免疫反應和上皮細胞增殖遷移。研究顯示，CXCL14在結直腸癌細胞中表達與肝轉移相關，可能通過增強血管內皮細胞增殖與遷移能力來增加血管新生，從而促進結直腸癌的肝轉移。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于探索CXCL14在免疫性疾病模型、腫瘤微環境動態變化、代謝調控網絡中的表達規律及其分子機制研究；可為探索CXCL14在細胞信號傳導、宿主 - 病原體互作或疾病動物模型中的功能提供可靠檢測工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Cxcl14 ELISA Kit; Bmac ELISA Kit; Kec ELISA Kit; Ks1 ELISA Kit; Mip2g ELISA Kit; Scyb14C-X-C motif chemokine 14 ELISA Kit; B-cell and monocyte-activating chemokine ELISA Kit; Chemokine BRAK ELISA Kit; Kidney-expressed chemokine CXC ELISA Kit; MIP-2G ELISA Kit; Small-inducible cytokine B14 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-92
    1:2 Average % 103
    Range % 99-108
    1:4 Average % 97
    Range % 92-101
    1:8 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of mouse CXCL14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 97 92-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.737 2.652 2.695 2.547
    10 2.235 2.276 2.256 2.108
    5 1.533 1.604 1.569 1.421
    2.5 0.915 0.938 0.927 0.779
    1.25 0.557 0.524 0.541 0.393
    0.625 0.312 0.327 0.320 0.172
    0.312 0.239 0.229 0.234 0.086
    0 0.146 0.150 0.148 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Chemotactic for CESS B-cells and THP-1 monocytes, but not T-cells.
  • 基因功能參考文獻：
    1. Platelets are a relevant source of CXCL14. Platelet-derived CXCL14 at the site of vascular lesions might play an important role in vascular repair/regeneration. PMID: 28359053
    2. CXCL14 Tg mice showed a suppressed rate of carcinogenesis, decreased tumour volume, and reduced pulmonary metastasis, as well as an increased survival rate of mice following tumour cell injection. PMID: 25765541
    3. CXCL14 does not seem to play a pivotal role during influenza and Escherichia coli infections of the lung. PMID: 25313607
    4. CXCL14 was able to promote bone metastasis through enhancement of cancer cell tropism to the bone and/or recruitment of bone marrow cells around metastatic cancer cells. PMID: 24534874
    5. In conclusion, our results suggested the important function of Cxcl14 in uNK cells and the proper level of Cxcl14 protein were required to recruit NK cells to pregnant uterus. PMID: 23688424
    6. the transient expression of CXCL14 by Purkinje cells in the developing cerebellum, suggesting that it must be involved in the postnatal maturation of the cerebellum PMID: 22843118
    7. CXCL14 may play an important role in central nervous system regulation of feeding behavior PMID: 20428232
    8. Study identifies CXCL14 as a novel marker of tendon connective tissue. PMID: 21038449
    9. Murine CXCL14 is dispensable for the homeostatic recruitment of antigen-presenting cells toward the periphery and for LC functionality. PMID: 17130243
    10. CXCL14 is a critical chemoattractant of white adipose tissue macrophages and a novel regulator of glucose metabolism that functions mainly in skeletal muscle. PMID: 17724031
    11. These results suggest that CXCL14 plays a causal role in high-fat diet-induced obesity. PMID: 17971304
    12. Data suggest that despite the structural homology and similarity in tissue distribution of human and murine CXCL14, distinct differences point to diverse, species-specific needs for CXCL14 in epithelial immunity. PMID: 18809336
    13. findings demonstrate that early overexpression of PMP22 in a mouse model of Charcot-Marie-Tooth disease type 1A results in a strong up-regulation of CXCL14 which seems to play a novel regulatory role in Schwann cell differentiation PMID: 19111616
    14. CXCL14 is an important paracrine/autocrine modulator regulating trophoblast outgrowth at the maternal-fetal interface during the process of pregnancy establishment. PMID: 19626669
    15. These data indicate the possibility that BRAK expression inhibits tumor cell establishment by regulating interactions between tumor stem cells and NK cells and/or suppressing formation of tumor microvessels. PMID: 19887729

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 組織特異性：
    Highly expressed in brain, lung, ovary, muscle and in kidney and liver parenchyma, and at lower levels in bone marrow.
  • 數據庫鏈接：


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