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Mouse Bone-specific alkaline phosphatase (BALP) ELISA kit

  • 中文名稱:
    小鼠骨特性堿性磷酸酶(BALP)酶聯免疫試劑盒
  • 貨號:
    CSB-E11914m
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠骨特性堿性磷酸酶(BALP)酶聯免疫試劑盒(CSB-E11914m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的BALP含量。BALP即骨堿性磷酸酶,是成骨細胞的特異性標志物。其背景在于反映骨代謝情況,在骨骼生長、修復等過程有重要意義。研究機制主要是通過檢測其活性,以評估骨轉換狀態,輔助診斷兒童佝僂病、骨質疏松等骨代謝相關疾病。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL,該產品可廣泛應用于骨骼發育研究、骨質疏松模型構建、骨修復材料效果評估等科研領域,尤其適用于分析藥物干預或基因修飾對骨代謝影響的實驗中。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Alpl ELISA Kit; Akp-2 ELISA Kit; Akp2 ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase 2 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
  • 縮寫:
    BALP
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.25 ng/mL-80 ng/mL
  • 靈敏度:
    0.31 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:5Average %99
    Range %94-104
    1:10Average %92
    Range %88-98
    1:20Average %84
    Range %80-88
    1:40Average %97
    Range %92-102
  • 回收率:
    The recovery of mouse BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9592-98
    EDTA plasma (n=4)9287-97
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.912 2.811 2.862 2.707
    402.321 2.203 2.262 2.107
    201.468 1.447 1.458 1.303
    100.877 0.866 0.872 0.717
    50.522 0.534 0.528 0.373
    2.50.350 0.362 0.356 0.201
    1.250.259 0.267 0.263 0.108
    00.154 0.155 0.155  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
  • 基因功能參考文獻:
    1. intestinal alkaline phosphatase knockout mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone. PMID: 29234952
    2. Study results from cerulein induced pancreatitis model in TNAP+/- mice show that altered TNAP expression results in heightened pancreatic inflammation, which may be explained by an augmented response of neutrophils and by a higher sensitivity of acinar cells to cerulein injury. PMID: 30251694
    3. TNAP activation in vascular smooth muscle cells (VSMCs) appears sufficient to induce calcification. TNAP activation in VSMCs stimulates expression of chondrocyte markers. PMID: 27932058
    4. Our results offer clear evidence that TNAP modulates T lymphocyte function and specifically T cell-dependent colitis. PMID: 28039309
    5. The results are the first to demonstrate a role for ENC1 in the control of osteoblast differentiation. Additionally, the contrasting mineralization phenotypes and transcriptional patterns seen with coordinate knockdown of both ENC1 isoforms vs selective knockdown of 67 kDa ENC1 suggest opposing roles for the isoforms in regulation of osteoblastic differentiation, through effects on Alpl expression and phosphate cellular PMID: 27996212
    6. TNAP overexpression in vascular endothelium in mice leads to an unusual course of coronary atherosclerosis and was accompanied by the reduction in body weight and left ventricular ejection fraction. PMID: 29023576
    7. Data, including data from studies using cells from transgenic/knockout mice, suggest that Med1 plays role in enamel formation; Med1 induces Alpl via stimulation of Notch1 signaling by forming Notch1-RBP-Jk complex on Alpl promoter. (Med1 = mediator complex subunit 1; Alpl = alkaline phosphatase, liver-bone-kidney; Notch1 = Notch gene homolog 1; RBP-Jk = kappa J region recombining binding protein suppressor of hairless) PMID: 28673966
    8. These analyses revealed that TNAP deficient mice present an increased proliferation of neural precursors, an altered neuronal morphology, and an augmented neuronal activity. We found that these alterations were associated with a partial downregulation of the purinergic P2X7 receptor (P2X7R). PMID: 27466191
    9. Despite similar deficiencies in alkaline phosphatase, Alpl(-/-) mice develop craniosynostosis and a brachycephalic/acrocephalic craniofacial shape of variable penetrance. PMID: 26605996
    10. Prevention of lethal murine hypophosphatasia by neonatal ex vivo gene therapy using lentivirally transduced bone marrow cells expressing Akp-2. PMID: 26467745
    11. TNAP in the vasculature contributes to the pathology of medial vascular calcification and that it is a druggable target. PMID: 25428889
    12. In cardiac fibroblasts, TNAP expression and activity is induced by sFRP2. PMID: 25972450
    13. p107 is required for the efficient recruitment of an activating SWI/SNF chromatin-remodeling complex, an essential event in Alpl induction. PMID: 25182511
    14. Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
    15. Findings demonstrate that Alpl(-/-) mice exhibit a craniofacial skeletal phenotype similar to that seen in infants with HPP, including true bony craniosynostosis in the context of severely diminished bone mineralization PMID: 25014884
    16. CD73 and TNAP play interactive roles to metabolize luminally applied 5'-AMP in the renal vasculature such that inhibition of both is required to inhibit the production of adenosine. PMID: 24990899
    17. TNAP plays a role in governing the phosphorylation status of phospholamban in the sarcoplasmic reticulum. PMID: 25015959
    18. Taken together, these data indicate that ATF3 is a novel negative regulator of osteoblast differentiation by specifically suppressing ALP gene expression in preosteoblasts. PMID: 24315873
    19. data suggest that the promineralization role of TNAP may be related not only to its accepted pyrophosphatase activity but also to its ability to modify the phosphorylation status of OPN. PMID: 23427088
    20. mineralization abnormalities of dentin; reduced overall mineralization with decreased matrix vesicle mineralization in the Phospho1(-/-) mice; almost complete absence of matrix vesicles in mice; further reduction in mineralization. PMID: 23694930
    21. In the cerebral cortex, myelinated axons, while present in wild-type, were absent in the Akp2( -/- ) mice and these animals also displayed a significantly increased proportion of immature cortical synapses. PMID: 22696173
    22. a link between ATRA-induced mL/B/K-ALP gene transcription and chromatin remodeling PMID: 22270475
    23. Results demonstrate that TNAP, regulating both ligand availability and protein expression of P2X7 receptor, is essential for axonal development. PMID: 21289095
    24. Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615
    25. We show that TNAP knockdown reduces cell proliferation and differentiation into neurons or oligodendrocytes PMID: 20849921
    26. These results demonstrate that calcium ions released from apatite are important in the synergistic effect of 20alpha-HC and apatite. PMID: 20683129
    27. Once the HT2B receptor is expressed, it constitutively controls tissue-nonspecific alkaline phosphatase activity at a post-translational level along the overall period of bone mineral deposition PMID: 20573958
    28. Tissue-nonspecific alkaline phosphatase is the enzyme that hydrolyzes both ATP and inorganic pyrophosate in matrix vesicles. PMID: 19874193
    29. correction of bone mineralization abnormalities in knockout mice null for both the TNAP (Akp2) and PC-1 (Enpp1) genes PMID: 12082181
    30. The Alpl (Akp2) gene is located within the quantitative trait locus region for alkaline phosphatase activity on chromosome 4. PMID: 16159911
    31. alkaline phosphatase 2(Hpp/Hpp) mice develop late-onset skeletal disease, notably defective endochondral ossification and bone mineralization that leads to arthropathies of knees and shoulders PMID: 17539739
    32. The process selectively internalizes IAP and may contribute to the appearance of the enzyme in serum and surfactant-like particles. PMID: 17947448
    33. Results show that inorganic phosphate (P(i)) levels and TNSALP activity increased in response to androgen/androgen receptor (AR), and P(i) signals increase the expression and translocation of AR. PMID: 18838539
    34. TNAP-AID mice expressed alpha-fetoprotein and had deleterious mutations in the tumour suppressor gene Trp53, some of which corresponded to those found in human cancer. PMID: 18997814

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  • 亞細胞定位:
    Cell membrane; Lipid-anchor, GPI-anchor. Extracellular vesicle membrane; Lipid-anchor, GPI-anchor. Mitochondrion membrane; Lipid-anchor, GPI-anchor. Mitochondrion intermembrane space.
  • 蛋白家族:
    Alkaline phosphatase family
  • 組織特異性:
    Widely expressed. Expressed in DRG neurons and spinal cord neurons.
  • 數據庫鏈接:


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