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Mouse Bone marrow proteoglycan(PRG2) ELISA kit

  • 中文名稱:
    小鼠骨髓蛋白多糖(PRG2)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL018670MO
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠骨髓蛋白多糖(PRG2)酶聯(lián)免疫試劑盒(CSB-EL018670MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PRG2含量。PRG2即前顆粒蛋白 2,是一類具有潛在藥用價值的靶點。其與過敏、炎癥反應(yīng)等密切相關(guān),研究機制主要是通過對免疫細胞的調(diào)節(jié),在免疫信號傳導通路中發(fā)揮作用,目前對其深入研究或可為相關(guān)疾病治療帶來新策略。試劑盒檢測范圍為3.9 ng/mL-250 ng/mL,廣泛應(yīng)用于骨髓發(fā)育機制研究、炎癥性疾病模型評估或血液相關(guān)病理變化的分子機制探索本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Prg2 ELISA Kit; Mbp-1 ELISA Kit; Bone marrow proteoglycan ELISA Kit; BMPG ELISA Kit; Proteoglycan 2) [Cleaved into: Eosinophil granule major basic protein ELISA Kit; EMBP ELISA Kit; MBP)] ELISA Kit
  • 縮寫:
    PRG2
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    3.9 ng/mL-250 ng/mL
  • 靈敏度:
    0.98 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %94
    Range %89-97
    1:10Average %103
    Range %98-107
    1:20Average %90
    Range %84-94
    1:40Average %98
    Range %92-102
  • 回收率:
    The recovery of mouse PRG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-96
    EDTA plasma (n=4)9488-97
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2502.772 2.664 2.718 2.597
    1252.026 2.076 2.051 1.930
    62.51.218 1.200 1.209 1.088
    31.250.677 0.654 0.666 0.545
    15.60.392 0.387 0.390 0.269
    7.80.299 0.287 0.293 0.172
    3.90.208 0.203 0.206 0.085
    00.122 0.120 0.121
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Cytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions.
  • 基因功能參考文獻:
    1. data show that bioactive phospholipids and PRG-2 are critical for guiding thalamic axons to their proper cortical targets. PMID: 27641493
    2. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
    3. major basic protein decreases epithelial barrier function and in this manner contributes to the pathogenesis of inflammatory bowel diseases. PMID: 16227527
    4. Host protection against Brugia pahangi infections was unimpaired in mice deficient in major basic protein 1. PMID: 16299347
    5. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice.(major basic protein 1) PMID: 16926417
    6. Eosinophil-derived MBP-1 plays a significant role in regulating muscular dystrophy by attenuating the cellular immune response and promoting tissue fibrosis that can eventually contribute to increased mortality. PMID: 18430716

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  • 亞細胞定位:
    Cytoplasmic granule.
  • 數(shù)據(jù)庫鏈接:


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