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  4. Mouse Aspartate Aminotransferase (AST) ELISA kit

Mouse Aspartate Aminotransferase (AST) ELISA kit

  • 中文名稱：
    小鼠天門冬氨酸氨基轉移酶(AST)酶聯免疫試劑盒
  • 貨號：
    CSB-E12649m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠天門冬氨酸氨基轉移酶(AST)酶聯免疫試劑盒（CSB-E12649m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的GOT2含量。GOT2是重要靶點。它是一種氨基轉移酶，參與細胞內的氨基代謝。研究發現其在腫瘤代謝重編程中發揮關鍵作用，通過調節谷氨酰胺代謝等途徑支持腫瘤細胞生長、增殖及存活，有望成為腫瘤等疾病治療的新方向。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，適用于實驗室中肝毒性藥物篩選、心臟疾病模型建立、細胞損傷機制研究等場景，例如通過檢測細胞培養上清中的 AST 釋放量評價化合物對肝實質細胞的毒性作用，或在小鼠代謝性疾病模型中動態監測血漿 AST 水平變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Got2 ELISA kit; Got-2Aspartate aminotransferase ELISA kit; mitochondrial ELISA kit; mAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.7 ELISA kit; Fatty acid-binding protein ELISA kit; FABP-1 ELISA kit; Glutamate oxaloacetate transaminase 2 ELISA kit; Kynurenine aminotransferase 4 ELISA kit; Kynurenine aminotransferase IV ELISA kit; Kynurenine--oxoglutarate transaminase 4 ELISA kit; Kynurenine--oxoglutarate transaminase IV ELISA kit; Plasma membrane-associated fatty acid-binding protein ELISA kit; FABPpm ELISA kit; Transaminase A ELISA kit
  • 縮寫：
    GOT2
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    1.56 mIU/mL-100 mIU/mL
  • 靈敏度：
    0.39 mIU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %85-95
    1:2Average %93
    Range %87-98
    1:4Average %91
    Range %89-94
    1:8Average %94
    Range %90-98
  • 回收率：
    The recovery of mouse AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-99
    EDTA plasma (n=4)9894-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    1002.915 2.898 2.907 2.720
    502.475 2.432 2.454 2.267
    252.015 2.002 2.009 1.822
    12.51.376 1.342 1.359 1.172
    6.250.731 0.717 0.724 0.537
    3.120.472 0.445 0.459 0.272
    1.560.312 0.301 0.307 0.120
    00.189 0.185 0.187 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse AST antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled AST antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
  • 基因功能參考文獻：
    1. GOT2-mediated ATP synthesis may be a pivotal mechanism underlying the protective effect of febuxostat against neurodegeneration in EA PMID: 29107957
    2. it is quite likely that the down regulation of GOT2 after blast exposure is playing a significant role in mitochondrial dysfunction after blast exposure PMID: 23600763
    3. The role of mAspAT in vivo is to control the levels of endogenous kynurenic acid (KYNA) through modulation of the mAspAt in the mouse brain. PMID: 20977429
    4. KAT IV inhibited by equimolar tryptophan and aspartate, and half-life is 6.5 minutes at 65 degree C. PMID: 20482848
  • 亞細胞定位：
    Mitochondrion matrix. Cell membrane.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 組織特異性：
    Detected in brain (at protein level).
  • 數據庫鏈接：


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