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Mouse Anti-Smooth Muscle Antibody,ASMA ELISA Kit

  • 中文名稱:
    小鼠抗平滑肌抗體(ASMA)酶聯免疫試劑盒
  • 貨號:
    CSB-E08795m
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠抗平滑肌抗體(ASMA)酶聯免疫試劑盒(CSB-E08795m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ASM Ab含量。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,該產品可為研究者提供小鼠模型中ASMA水平的動態監測,支持自身免疫性疾病機制探索、藥物干預效果評估及病理模型構建等科研需求,尤其適用于肝臟、血管相關疾病動物實驗的樣本分析。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    ASM Ab
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ASMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 98
    Range % 87-102
    1:2 Average % 98
    Range % 90-101
    1:4 Average % 89
    Range % 84-93
    1:8 Average % 107
    Range % 98-111
  • 回收率:
    The recovery of mouse ASMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 81-94
    EDTA plasma (n=4) 114 102-117
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    100 2.561 2.498 2.530 2.397
    50 1.413 1.456 1.435 1.302
    25 0.695 0.702 0.699 0.566
    12.5 0.409 0.417 0.413 0.280
    6.25 0.292 0.304 0.298 0.165
    3.12 0.190 0.198 0.194 0.061
    1.56 0.166 0.171 0.169 0.036
    0 0.136 0.130 0.133  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

I want to know if the secondary antibody in this kit is raised
against mouse IgG.

A:
Thanks for your inquiry.
Mouse Anti-Smooth Muscle Antibody,ASMA ELISA KitCSB-E08795m
This kit is for quantitative determination of Anti-Smooth Muscle Antibody.
There is no antibody in the kit.
I will send you the kit manual by email.
Q:

Can you please let me know what is dilution range you used for mouse serum ( range is not provided in the protocol).

A:
Thanks for your inquiry. If dilution range is not provided in the protocol, it means that dilution is not needed. We suggest you do a pretest first.
Q:

Thanks for your reply. Okay, does that mean I need 100ul of serum sample? (I realize that dilution depends on my samples). If you can get any data on sample dilution that it would be very helpful.

A:
We tested undiluted serum and plasma samples before and the test value is 25ng/ml. The sample volume is 100ul.
You can do a pretest to confirm the optimal dilution factor according to your sample's situation.


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