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Mouse Angiopoietin-related protein 7(ANGPTL7) ELISA kit

  • 中文名稱:
    小鼠血管生成素相關蛋白7(ANGPTL7)酶聯免疫試劑盒
  • 貨號:
    CSB-EL001715MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠血管生成素相關蛋白7(ANGPTL7)酶聯免疫試劑盒(CSB-EL001715MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ANGPTL7含量。ANGPTL7是一個靶點。其背景上,它可能與某些生理或病理過程相關。在研究機制方面,目前可能著重探索其在疾病發生發展中的作用,比如對血管生成、細胞增殖等的影響,后續研究或為相關疾病治療提供新的方向和策略。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL,本試劑盒適用于小鼠模型中ANGPTL7蛋白水平的定量分析,可應用于心血管疾病、代謝調控或組織纖維化等領域的機制研究,例如在基因編輯模型或藥物干預實驗中評估ANGPTL7的動態變化。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Angptl7Angiopoietin-related protein 7 ELISA kit; Angiopoietin-like protein 7 ELISA kit
  • 縮寫:
    ANGPTL7
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    6.25 pg/mL-400 pg/mL
  • 靈敏度:
    1.56 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-94
    1:2Average %98
    Range %93-103
    1:4Average %102
    Range %95-109
    1:8Average %94
    Range %90-98
  • 回收率:
    The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-101
    EDTA plasma (n=4)9388-98
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.758 2.890 2.824 2.632
    2001.823 1.951 1.887 1.695
    1001.077 1.138 1.108 0.916
    500.644 0.621 0.633 0.441
    250.467 0.451 0.459 0.267
    12.50.335 0.336 0.336 0.144
    6.250.262 0.255 0.259 0.067
    00.184 0.199 0.192
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
  • 基因功能參考文獻:
    1. Angptl7 is a factor that promotes pro-inflammatory responses in macrophages through the P38 MAPK signaling pathway PMID: 26973239
    2. Angptl7 was indispensable for bone marrow microenvironment to support hematopoietic stem cells repopulation PMID: 25652910
  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:


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