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  4. Mouse Aminopeptidase N(ANPEP) ELISA kit

Mouse Aminopeptidase N(ANPEP) ELISA kit

  • 中文名稱(chēng)：
    鼠氨肽酶N(ANPEP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001827MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    鼠氨肽酶N(ANPEP)酶聯(lián)免疫試劑盒（CSB-EL001827MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ANPEP含量。ANPEP 即氨肽酶 N，是一種膜結(jié)合蛋白。其作為重要靶點(diǎn)，在腫瘤血管生成、細(xì)胞遷移和侵襲等過(guò)程中發(fā)揮作用。研究發(fā)現(xiàn)它可調(diào)節(jié)多種生物活性肽功能。在腫瘤領(lǐng)域，其高表達(dá)與腫瘤進(jìn)展、轉(zhuǎn)移相關(guān)，有望成為腫瘤診斷和治療新方向。試劑盒檢測(cè)范圍為39 mU/mL-2500 mU/mL，科研領(lǐng)域可應(yīng)用于炎癥性疾病模型評(píng)估、腫瘤微環(huán)境研究、腸道屏障功能分析及藥物干預(yù)實(shí)驗(yàn)中的酶活性動(dòng)態(tài)監(jiān)測(cè)，為探索ANPEP在免疫調(diào)節(jié)、組織修復(fù)等分子機(jī)制中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Anpep ELISA kit; Lap-1 ELISA kit; Lap1Aminopeptidase N ELISA kit; AP-N ELISA kit; mAPN ELISA kit; EC 3.4.11.2 ELISA kit; Alanyl aminopeptidase ELISA kit; Aminopeptidase M ELISA kit; AP-M ELISA kit; Membrane protein p161 ELISA kit; Microsomal aminopeptidase ELISA kit; CD antigen CD13 ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    39 mU/mL-2500 mU/mL
  • 靈敏度：
    9.7 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 84-97  
    1:2 Average % 95  
    Range % 90-99  
    1:4 Average % 102  
    Range % 95-106  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of mouse ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 82-96  
    EDTA plasma (n=4) 97 93-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    2500 2.500 2.400 2.450 2.285  
    1250 1.970 1.830 1.900 1.735  
    625 1.119 1.075 1.097 0.932  
    312.5 0.706 0.717 0.712 0.547  
    156.25 0.447 0.458 0.453 0.288  
    78 0.304 0.315 0.310 0.145  
    39 0.246 0.252 0.249 0.084  
    0 0.164 0.165 0.165 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.
  • 基因功能參考文獻(xiàn)：
    1. we concluded that APN plays a significant role in the regulation of several sperm functions and early embryonic development. In addition, increased APN activity could potentially lead to several adverse consequences related to male fertility. PMID: 28859152
    2. These studies identified CD13 as a novel negative regulator of mast cell activation in vitro and in vivo. PMID: 26936360
    3. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
    4. Alanyl aminopeptidase expression is confined to mature zymogenic chief cells and its expression is lost en route to metaplasia. PMID: 26514774
    5. CD13 negatively regulates TLR4 signaling, thereby balancing the innate response by maintaining the inflammatory equilibrium critical to innate immune regulation. PMID: 25801433
    6. The impact of loss of CD13 on a model of ischemic skeletal muscle injury, was investigated. PMID: 24307555
    7. Molecular mechanisms regulating CD13-mediated adhesion. PMID: 24627994
    8. CD13 is essential for proper trafficking of the inflammatory cells necessary to prime and sustain the reparative response, thus promoting optimal post-infarction healing. PMID: 23761403
    9. Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking. PMID: 23997214
    10. This indicates that Anpep plays a critical role in the proteolytic remodelling of mammary tissue during adult mammary development. PMID: 22983824
    11. the presence of digestive protein complexes in the intestinal brush-border containing the peptidases APN and ACE2 and the neutral amino acid transporter B0AT1 PMID: 22677001
    12. cooperation in APN expression by both cancer cells and nonmalignant stromal cells within the tumor microenvironment promotes angiogenesis, tumor growth, and metastasis PMID: 22307623
    13. expression of CD13 in normal and pathological human tissues PMID: 21339174
    14. Data show that the majority of chondrocyte-like cells are of bone marrow origin, whereas CD34(+)/CD13(+) myeloid precursors appear to infiltrate the plaque actively and transdifferentiate into chondrocyte-like cells in the progression of atherosclerosis. PMID: 20489139
    15. although CD13 is highly expressed on myeloid cells and is a reliable marker of the myeloid lineage of normal and leukemic cells, it is not a critical regulator of hematopoietic development, hemostasis, or myeloid cell function PMID: 20430777
    16. CD13 expressed in microglia-derived exosomes is active in neuropeptide degradation and could have a distinct effect on local neuropeptide activity in the brain. PMID: 16081791
    17. Results suggest that CD26 and CD13 play a role in T cell function in tissue-specific autoimmunity in the central nervous system. PMID: 16700521
    18. In cardiac angiogenesis endothelial CD13/APN is upregulated. It can be targeted specifically with cNGR conjugates. In the heart cNGR binds its endothelial target only in angiogenic areas. PMID: 16990557
    19. aminopeptidase N has a role in angiogenesis PMID: 17360568

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase M1 family
  • 組織特異性：
    Expressed in the intestinal brush border (at protein level). Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:16790

    STRING: 10090.ENSMUSP00000035943

    UniGene: Mm.4487



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